Strain Fitness in Pseudomonas fluorescens GW456-L13 around PfGW456L13_174

Experiment: nitrate 200 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPfGW456L13_173 and PfGW456L13_174 are separated by 252 nucleotidesPfGW456L13_174 and PfGW456L13_175 are separated by 175 nucleotides PfGW456L13_173 - HAMP domain protein, at 197,775 to 199,292 _173 PfGW456L13_174 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 199,545 to 200,258 _174 PfGW456L13_175 - Topoisomerase IV subunit A (EC 5.99.1.-), at 200,434 to 202,701 _175 Position (kb) 199 200 201Strain fitness (log2 ratio) -2 -1 0 1 2at 199.290 kb on + strandat 199.291 kb on - strandat 199.291 kb on - strandat 199.475 kb on + strandat 199.475 kb on + strandat 199.476 kb on - strandat 199.476 kb on - strandat 199.479 kb on + strandat 199.479 kb on + strandat 199.479 kb on + strandat 199.479 kb on + strandat 199.479 kb on + strandat 199.480 kb on - strandat 199.480 kb on - strandat 199.527 kb on + strandat 199.527 kb on + strandat 199.528 kb on - strandat 199.528 kb on - strandat 199.546 kb on + strandat 199.546 kb on + strandat 199.546 kb on + strandat 199.547 kb on - strandat 199.547 kb on - strandat 199.547 kb on - strandat 199.547 kb on - strandat 199.547 kb on - strandat 199.547 kb on - strandat 199.572 kb on - strandat 199.572 kb on - strandat 199.578 kb on - strandat 199.578 kb on - strandat 199.617 kb on + strand, within PfGW456L13_174at 199.618 kb on - strand, within PfGW456L13_174at 199.763 kb on + strand, within PfGW456L13_174at 199.942 kb on - strand, within PfGW456L13_174at 200.087 kb on - strand, within PfGW456L13_174at 200.115 kb on + strand, within PfGW456L13_174at 200.154 kb on - strand, within PfGW456L13_174at 200.170 kb on + strand, within PfGW456L13_174at 200.171 kb on - strand, within PfGW456L13_174at 200.171 kb on - strand, within PfGW456L13_174at 200.234 kb on + strandat 200.235 kb on - strandat 200.303 kb on - strandat 200.309 kb on + strandat 200.309 kb on + strandat 200.309 kb on + strandat 200.309 kb on + strandat 200.309 kb on + strandat 200.353 kb on - strandat 200.365 kb on + strandat 200.365 kb on + strandat 200.365 kb on + strandat 200.385 kb on + strandat 200.385 kb on + strandat 200.385 kb on + strandat 200.406 kb on - strandat 200.426 kb on + strandat 200.426 kb on + strandat 200.426 kb on + strandat 200.426 kb on + strandat 200.426 kb on + strandat 200.426 kb on + strandat 200.427 kb on - strandat 200.427 kb on - strandat 200.435 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrate 200 mM
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199,290 + +0.4
199,291 - -0.8
199,291 - +0.2
199,475 + -0.2
199,475 + -0.8
199,476 - -2.1
199,476 - -0.4
199,479 + +0.7
199,479 + -1.3
199,479 + -0.7
199,479 + -0.3
199,479 + +0.0
199,480 - -0.5
199,480 - -0.4
199,527 + -0.8
199,527 + +0.1
199,528 - -1.2
199,528 - -0.2
199,546 + -0.6
199,546 + +0.3
199,546 + -1.7
199,547 - -1.0
199,547 - -1.3
199,547 - -0.3
199,547 - +0.1
199,547 - +0.2
199,547 - -0.7
199,572 - -1.1
199,572 - -0.5
199,578 - -0.5
199,578 - -0.1
199,617 + PfGW456L13_174 0.10 -1.6
199,618 - PfGW456L13_174 0.10 -0.4
199,763 + PfGW456L13_174 0.31 -0.8
199,942 - PfGW456L13_174 0.56 -0.2
200,087 - PfGW456L13_174 0.76 -0.4
200,115 + PfGW456L13_174 0.80 -1.0
200,154 - PfGW456L13_174 0.85 -1.0
200,170 + PfGW456L13_174 0.88 -0.8
200,171 - PfGW456L13_174 0.88 -0.4
200,171 - PfGW456L13_174 0.88 -0.4
200,234 + -1.1
200,235 - -1.1
200,303 - +1.0
200,309 + -0.8
200,309 + +0.8
200,309 + -1.5
200,309 + -2.4
200,309 + +0.3
200,353 - +1.7
200,365 + +0.1
200,365 + -1.3
200,365 + -1.5
200,385 + -1.1
200,385 + -0.7
200,385 + -0.9
200,406 - +0.9
200,426 + -1.0
200,426 + -1.8
200,426 + -1.1
200,426 + -0.4
200,426 + -0.7
200,426 + -1.1
200,427 - +2.3
200,427 - +1.6
200,435 + +0.1

Or see this region's nucleotide sequence