Experiment: nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_1722 and PfGW456L13_1723 are separated by 133 nucleotides PfGW456L13_1723 and PfGW456L13_1724 are separated by 66 nucleotides
PfGW456L13_1722 - Thiol:disulfide interchange protein DsbC, at 1,897,044 to 1,897,775
_1722
PfGW456L13_1723 - Homoserine dehydrogenase (EC 1.1.1.3), at 1,897,909 to 1,899,213
_1723
PfGW456L13_1724 - Threonine synthase (EC 4.2.3.1), at 1,899,280 to 1,900,689
_1724
Position (kb)
1897
1898
1899
1900 Strain fitness (log2 ratio)
-2
-1
0
1 at 1896.944 kb on + strand at 1896.944 kb on + strand at 1896.944 kb on + strand at 1896.944 kb on + strand at 1896.944 kb on + strand at 1896.944 kb on + strand at 1896.945 kb on - strand at 1896.945 kb on - strand at 1896.945 kb on - strand at 1896.945 kb on - strand at 1896.952 kb on - strand at 1896.981 kb on + strand at 1896.981 kb on + strand at 1897.133 kb on + strand, within PfGW456L13_1722 at 1897.133 kb on + strand, within PfGW456L13_1722 at 1897.133 kb on + strand, within PfGW456L13_1722 at 1897.203 kb on + strand, within PfGW456L13_1722 at 1897.257 kb on + strand, within PfGW456L13_1722 at 1897.257 kb on + strand, within PfGW456L13_1722 at 1897.257 kb on + strand, within PfGW456L13_1722 at 1897.272 kb on + strand, within PfGW456L13_1722 at 1897.272 kb on + strand, within PfGW456L13_1722 at 1897.417 kb on + strand, within PfGW456L13_1722 at 1897.417 kb on + strand, within PfGW456L13_1722 at 1897.417 kb on + strand, within PfGW456L13_1722 at 1897.417 kb on + strand, within PfGW456L13_1722 at 1897.418 kb on - strand, within PfGW456L13_1722 at 1897.418 kb on - strand, within PfGW456L13_1722 at 1897.418 kb on - strand, within PfGW456L13_1722 at 1897.500 kb on + strand, within PfGW456L13_1722 at 1897.500 kb on + strand, within PfGW456L13_1722 at 1897.500 kb on + strand, within PfGW456L13_1722 at 1897.501 kb on - strand, within PfGW456L13_1722 at 1897.501 kb on - strand, within PfGW456L13_1722 at 1897.773 kb on + strand at 1897.840 kb on + strand at 1899.211 kb on + strand at 1899.211 kb on + strand at 1899.211 kb on + strand at 1899.211 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 200 mM remove 1,896,944 + +0.8 1,896,944 + +0.4 1,896,944 + -0.6 1,896,944 + +0.7 1,896,944 + +1.0 1,896,944 + +0.2 1,896,945 - +0.0 1,896,945 - -0.3 1,896,945 - +0.4 1,896,945 - +0.9 1,896,952 - -1.3 1,896,981 + -0.2 1,896,981 + -2.1 1,897,133 + PfGW456L13_1722 0.12 +0.1 1,897,133 + PfGW456L13_1722 0.12 -1.6 1,897,133 + PfGW456L13_1722 0.12 -1.8 1,897,203 + PfGW456L13_1722 0.22 -0.5 1,897,257 + PfGW456L13_1722 0.29 -0.5 1,897,257 + PfGW456L13_1722 0.29 -0.7 1,897,257 + PfGW456L13_1722 0.29 -0.9 1,897,272 + PfGW456L13_1722 0.31 -0.7 1,897,272 + PfGW456L13_1722 0.31 -0.4 1,897,417 + PfGW456L13_1722 0.51 -1.3 1,897,417 + PfGW456L13_1722 0.51 +0.6 1,897,417 + PfGW456L13_1722 0.51 -0.9 1,897,417 + PfGW456L13_1722 0.51 -0.6 1,897,418 - PfGW456L13_1722 0.51 -1.8 1,897,418 - PfGW456L13_1722 0.51 -0.2 1,897,418 - PfGW456L13_1722 0.51 -0.3 1,897,500 + PfGW456L13_1722 0.62 +0.5 1,897,500 + PfGW456L13_1722 0.62 +0.0 1,897,500 + PfGW456L13_1722 0.62 -0.5 1,897,501 - PfGW456L13_1722 0.62 +0.7 1,897,501 - PfGW456L13_1722 0.62 +0.0 1,897,773 + -1.2 1,897,840 + +1.3 1,899,211 + +0.8 1,899,211 + -0.3 1,899,211 + +0.2 1,899,211 + +0.3
Or see this region's nucleotide sequence