Experiment: nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_156 and PfGW456L13_157 overlap by 4 nucleotides PfGW456L13_157 and PfGW456L13_158 are separated by 15 nucleotides PfGW456L13_158 and PfGW456L13_159 overlap by 13 nucleotides
PfGW456L13_156 - DNA mismatch repair protein MutL, at 179,934 to 181,844
_156
PfGW456L13_157 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 181,841 to 183,256
_157
PfGW456L13_158 - TsaE protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA, at 183,272 to 183,742
_158
PfGW456L13_159 - NAD(P)HX epimerase / NAD(P)HX dehydratase, at 183,730 to 185,229
_159
Position (kb) 181
182
183
184 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 180.857 kb on - strand, within PfGW456L13_156 at 181.061 kb on - strand, within PfGW456L13_156 at 181.113 kb on + strand, within PfGW456L13_156 at 181.114 kb on - strand, within PfGW456L13_156 at 181.114 kb on - strand, within PfGW456L13_156 at 181.114 kb on - strand, within PfGW456L13_156 at 181.114 kb on - strand, within PfGW456L13_156 at 182.046 kb on + strand, within PfGW456L13_157 at 182.047 kb on - strand, within PfGW456L13_157 at 182.047 kb on - strand, within PfGW456L13_157 at 182.047 kb on - strand, within PfGW456L13_157 at 183.257 kb on - strand at 183.257 kb on - strand at 183.274 kb on - strand at 183.274 kb on - strand at 183.274 kb on - strand at 183.274 kb on - strand at 183.274 kb on - strand at 184.030 kb on - strand, within PfGW456L13_159 at 184.030 kb on - strand, within PfGW456L13_159
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 200 mM remove 180,857 - PfGW456L13_156 0.48 +0.2 181,061 - PfGW456L13_156 0.59 -0.5 181,113 + PfGW456L13_156 0.62 +0.1 181,114 - PfGW456L13_156 0.62 -0.5 181,114 - PfGW456L13_156 0.62 -0.4 181,114 - PfGW456L13_156 0.62 +0.2 181,114 - PfGW456L13_156 0.62 +0.3 182,046 + PfGW456L13_157 0.14 -0.9 182,047 - PfGW456L13_157 0.15 -2.3 182,047 - PfGW456L13_157 0.15 -2.6 182,047 - PfGW456L13_157 0.15 -1.8 183,257 - -2.3 183,257 - -1.9 183,274 - -2.3 183,274 - -1.9 183,274 - -2.8 183,274 - -1.7 183,274 - -1.2 184,030 - PfGW456L13_159 0.20 +3.0 184,030 - PfGW456L13_159 0.20 +3.5
Or see this region's nucleotide sequence