Strain Fitness in Pseudomonas fluorescens GW456-L13 around PfGW456L13_143

Experiment: nitrate 200 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPfGW456L13_141 and PfGW456L13_142 are separated by 28 nucleotidesPfGW456L13_142 and PfGW456L13_143 are separated by 305 nucleotidesPfGW456L13_143 and PfGW456L13_144 overlap by 4 nucleotides PfGW456L13_141 - SSU ribosomal protein S18p @ SSU ribosomal protein S18p, zinc-independent, at 162,311 to 162,541 _141 PfGW456L13_142 - SSU ribosomal protein S6p, at 162,570 to 162,992 _142 PfGW456L13_143 - 23S rRNA (guanosine-2'-O-) -methyltransferase rlmB (EC 2.1.1.-), at 163,298 to 164,065 _143 PfGW456L13_144 - 3'-to-5' exoribonuclease RNase R, at 164,062 to 166,689 _144 Position (kb) 163 164 165Strain fitness (log2 ratio) -2 -1 0 1 2at 163.142 kb on + strandat 163.143 kb on - strandat 163.154 kb on - strandat 163.182 kb on + strandat 163.183 kb on - strandat 163.237 kb on + strandat 163.237 kb on + strandat 163.237 kb on + strandat 163.238 kb on - strandat 163.238 kb on - strandat 163.250 kb on + strandat 163.250 kb on + strandat 163.426 kb on - strand, within PfGW456L13_143at 163.512 kb on + strand, within PfGW456L13_143at 163.513 kb on - strand, within PfGW456L13_143at 163.513 kb on - strand, within PfGW456L13_143at 163.865 kb on + strand, within PfGW456L13_143at 163.865 kb on + strand, within PfGW456L13_143at 163.865 kb on + strand, within PfGW456L13_143at 163.865 kb on + strand, within PfGW456L13_143at 163.866 kb on - strand, within PfGW456L13_143at 164.027 kb on + strandat 164.027 kb on + strandat 164.027 kb on + strandat 164.028 kb on - strandat 164.028 kb on - strandat 164.028 kb on - strandat 164.036 kb on + strandat 164.036 kb on + strandat 164.036 kb on + strandat 164.036 kb on + strandat 164.036 kb on + strandat 164.036 kb on + strandat 164.036 kb on + strandat 164.037 kb on - strandat 164.037 kb on - strandat 164.037 kb on - strandat 164.037 kb on - strandat 164.037 kb on - strandat 164.037 kb on - strandat 164.037 kb on - strandat 164.037 kb on - strandat 164.043 kb on + strandat 164.043 kb on + strandat 164.043 kb on + strandat 164.043 kb on + strandat 164.044 kb on - strandat 164.044 kb on - strandat 164.044 kb on - strandat 164.077 kb on - strandat 164.134 kb on - strandat 164.317 kb on + strandat 164.317 kb on + strandat 164.317 kb on + strandat 164.318 kb on - strandat 164.577 kb on - strand, within PfGW456L13_144at 164.577 kb on - strand, within PfGW456L13_144at 164.579 kb on + strand, within PfGW456L13_144at 164.621 kb on + strand, within PfGW456L13_144at 164.621 kb on + strand, within PfGW456L13_144at 164.621 kb on + strand, within PfGW456L13_144at 164.621 kb on + strand, within PfGW456L13_144at 164.625 kb on + strand, within PfGW456L13_144at 164.626 kb on - strand, within PfGW456L13_144at 164.731 kb on + strand, within PfGW456L13_144at 164.731 kb on + strand, within PfGW456L13_144at 164.731 kb on + strand, within PfGW456L13_144at 164.731 kb on + strand, within PfGW456L13_144at 164.732 kb on - strand, within PfGW456L13_144at 164.732 kb on - strand, within PfGW456L13_144at 164.732 kb on - strand, within PfGW456L13_144at 164.954 kb on + strand, within PfGW456L13_144

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrate 200 mM
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163,142 + +0.0
163,143 - +0.4
163,154 - -0.5
163,182 + +0.6
163,183 - -0.3
163,237 + +0.6
163,237 + +0.4
163,237 + +0.3
163,238 - -0.9
163,238 - -1.4
163,250 + +0.2
163,250 + -0.0
163,426 - PfGW456L13_143 0.17 +0.4
163,512 + PfGW456L13_143 0.28 -1.6
163,513 - PfGW456L13_143 0.28 +0.6
163,513 - PfGW456L13_143 0.28 +0.3
163,865 + PfGW456L13_143 0.74 +0.8
163,865 + PfGW456L13_143 0.74 +0.6
163,865 + PfGW456L13_143 0.74 +0.5
163,865 + PfGW456L13_143 0.74 +0.6
163,866 - PfGW456L13_143 0.74 +1.3
164,027 + +0.1
164,027 + -0.3
164,027 + +0.4
164,028 - +0.7
164,028 - +0.4
164,028 - -0.1
164,036 + -0.1
164,036 + +0.3
164,036 + -0.0
164,036 + -0.5
164,036 + +0.3
164,036 + +0.5
164,036 + +1.4
164,037 - -0.2
164,037 - -1.0
164,037 - -1.5
164,037 - -0.3
164,037 - -0.5
164,037 - -0.3
164,037 - +0.2
164,037 - +0.2
164,043 + +0.3
164,043 + -0.4
164,043 + +0.3
164,043 + +0.8
164,044 - +1.0
164,044 - -1.1
164,044 - +0.6
164,077 - -0.3
164,134 - +0.4
164,317 + +0.8
164,317 + +0.2
164,317 + +1.9
164,318 - -0.3
164,577 - PfGW456L13_144 0.20 +0.5
164,577 - PfGW456L13_144 0.20 +1.6
164,579 + PfGW456L13_144 0.20 +0.1
164,621 + PfGW456L13_144 0.21 +0.8
164,621 + PfGW456L13_144 0.21 +1.2
164,621 + PfGW456L13_144 0.21 -1.9
164,621 + PfGW456L13_144 0.21 -0.1
164,625 + PfGW456L13_144 0.21 -0.6
164,626 - PfGW456L13_144 0.21 +1.0
164,731 + PfGW456L13_144 0.25 +1.2
164,731 + PfGW456L13_144 0.25 +1.7
164,731 + PfGW456L13_144 0.25 +1.7
164,731 + PfGW456L13_144 0.25 +1.5
164,732 - PfGW456L13_144 0.25 -0.1
164,732 - PfGW456L13_144 0.25 +0.6
164,732 - PfGW456L13_144 0.25 +0.7
164,954 + PfGW456L13_144 0.34 -0.4

Or see this region's nucleotide sequence