Experiment: nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_1197 and PfGW456L13_1198 are separated by 33 nucleotides PfGW456L13_1198 and PfGW456L13_1199 overlap by 1 nucleotides PfGW456L13_1199 and PfGW456L13_1200 are separated by 115 nucleotides PfGW456L13_1200 and PfGW456L13_1201 overlap by 4 nucleotides
PfGW456L13_1197 - Thiosulfate sulfurtransferase GlpE (EC 2.8.1.1), at 1,336,858 to 1,337,187
_1197
PfGW456L13_1198 - Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41), at 1,337,221 to 1,338,102
_1198
PfGW456L13_1199 - ApaG protein, at 1,338,102 to 1,338,482
_1199
PfGW456L13_1200 - SSU rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase (EC 2.1.1.182), at 1,338,598 to 1,339,407
_1200
PfGW456L13_1201 - 4-hydroxythreonine-4-phosphate dehydrogenase (EC 1.1.1.262), at 1,339,404 to 1,340,393
_1201
Position (kb)
1338
1339 Strain fitness (log2 ratio)
-1
0
1
2 at 1337.169 kb on + strand at 1337.169 kb on + strand at 1337.169 kb on + strand at 1337.169 kb on + strand at 1337.170 kb on - strand at 1337.170 kb on - strand at 1337.170 kb on - strand at 1337.170 kb on - strand at 1337.170 kb on - strand at 1337.170 kb on - strand at 1337.222 kb on + strand at 1337.275 kb on + strand at 1337.480 kb on + strand, within PfGW456L13_1198 at 1337.564 kb on + strand, within PfGW456L13_1198 at 1337.565 kb on - strand, within PfGW456L13_1198 at 1337.633 kb on + strand, within PfGW456L13_1198 at 1337.634 kb on - strand, within PfGW456L13_1198 at 1337.634 kb on - strand, within PfGW456L13_1198 at 1337.634 kb on - strand, within PfGW456L13_1198 at 1337.634 kb on - strand, within PfGW456L13_1198 at 1337.775 kb on - strand, within PfGW456L13_1198 at 1337.775 kb on - strand, within PfGW456L13_1198 at 1337.875 kb on - strand, within PfGW456L13_1198 at 1337.945 kb on + strand, within PfGW456L13_1198 at 1337.946 kb on - strand, within PfGW456L13_1198 at 1337.946 kb on - strand, within PfGW456L13_1198 at 1338.092 kb on + strand at 1338.092 kb on + strand at 1338.092 kb on + strand at 1338.092 kb on + strand at 1338.092 kb on + strand at 1338.092 kb on + strand at 1338.092 kb on + strand at 1338.092 kb on + strand at 1338.092 kb on + strand at 1338.092 kb on + strand at 1338.093 kb on - strand at 1338.093 kb on - strand at 1338.093 kb on - strand at 1338.093 kb on - strand at 1338.184 kb on + strand, within PfGW456L13_1199 at 1338.184 kb on + strand, within PfGW456L13_1199 at 1338.184 kb on + strand, within PfGW456L13_1199 at 1338.184 kb on + strand, within PfGW456L13_1199 at 1338.184 kb on + strand, within PfGW456L13_1199 at 1338.184 kb on + strand, within PfGW456L13_1199 at 1338.185 kb on - strand, within PfGW456L13_1199 at 1338.185 kb on - strand, within PfGW456L13_1199 at 1338.185 kb on - strand, within PfGW456L13_1199 at 1338.185 kb on - strand, within PfGW456L13_1199 at 1338.185 kb on - strand, within PfGW456L13_1199 at 1338.235 kb on + strand, within PfGW456L13_1199 at 1338.235 kb on + strand, within PfGW456L13_1199 at 1338.235 kb on + strand, within PfGW456L13_1199 at 1338.235 kb on + strand, within PfGW456L13_1199 at 1338.235 kb on + strand, within PfGW456L13_1199 at 1338.236 kb on - strand, within PfGW456L13_1199 at 1338.236 kb on - strand, within PfGW456L13_1199 at 1338.236 kb on - strand, within PfGW456L13_1199 at 1338.236 kb on - strand, within PfGW456L13_1199 at 1338.388 kb on + strand, within PfGW456L13_1199 at 1338.388 kb on + strand, within PfGW456L13_1199 at 1338.389 kb on - strand, within PfGW456L13_1199 at 1338.389 kb on - strand, within PfGW456L13_1199 at 1338.436 kb on + strand, within PfGW456L13_1199 at 1338.495 kb on - strand at 1338.519 kb on + strand at 1338.519 kb on + strand at 1338.520 kb on - strand at 1338.520 kb on - strand at 1338.520 kb on - strand at 1338.520 kb on - strand at 1338.538 kb on + strand at 1338.547 kb on + strand at 1338.554 kb on + strand at 1338.557 kb on + strand at 1338.557 kb on + strand at 1338.557 kb on + strand at 1338.558 kb on - strand at 1338.558 kb on - strand at 1338.558 kb on - strand at 1339.071 kb on - strand, within PfGW456L13_1200
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 200 mM remove 1,337,169 + +0.1 1,337,169 + +0.2 1,337,169 + -0.5 1,337,169 + -0.7 1,337,170 - +0.5 1,337,170 - +0.2 1,337,170 - +0.6 1,337,170 - -0.7 1,337,170 - -1.0 1,337,170 - +0.9 1,337,222 + -0.1 1,337,275 + -0.6 1,337,480 + PfGW456L13_1198 0.29 -0.4 1,337,564 + PfGW456L13_1198 0.39 +0.5 1,337,565 - PfGW456L13_1198 0.39 +0.0 1,337,633 + PfGW456L13_1198 0.47 +1.4 1,337,634 - PfGW456L13_1198 0.47 +1.1 1,337,634 - PfGW456L13_1198 0.47 +1.1 1,337,634 - PfGW456L13_1198 0.47 +1.1 1,337,634 - PfGW456L13_1198 0.47 -0.1 1,337,775 - PfGW456L13_1198 0.63 -1.2 1,337,775 - PfGW456L13_1198 0.63 +0.5 1,337,875 - PfGW456L13_1198 0.74 +1.3 1,337,945 + PfGW456L13_1198 0.82 +0.9 1,337,946 - PfGW456L13_1198 0.82 +0.6 1,337,946 - PfGW456L13_1198 0.82 +0.2 1,338,092 + +0.4 1,338,092 + -0.0 1,338,092 + -0.8 1,338,092 + +0.3 1,338,092 + +0.5 1,338,092 + +1.0 1,338,092 + +0.3 1,338,092 + +0.7 1,338,092 + -1.2 1,338,092 + +0.1 1,338,093 - +1.6 1,338,093 - +0.0 1,338,093 - +0.4 1,338,093 - +0.1 1,338,184 + PfGW456L13_1199 0.22 +0.7 1,338,184 + PfGW456L13_1199 0.22 +0.2 1,338,184 + PfGW456L13_1199 0.22 +1.3 1,338,184 + PfGW456L13_1199 0.22 -0.1 1,338,184 + PfGW456L13_1199 0.22 -0.1 1,338,184 + PfGW456L13_1199 0.22 +0.3 1,338,185 - PfGW456L13_1199 0.22 +0.4 1,338,185 - PfGW456L13_1199 0.22 -0.4 1,338,185 - PfGW456L13_1199 0.22 -0.0 1,338,185 - PfGW456L13_1199 0.22 +0.3 1,338,185 - PfGW456L13_1199 0.22 +0.7 1,338,235 + PfGW456L13_1199 0.35 +1.3 1,338,235 + PfGW456L13_1199 0.35 +0.6 1,338,235 + PfGW456L13_1199 0.35 -0.6 1,338,235 + PfGW456L13_1199 0.35 +1.0 1,338,235 + PfGW456L13_1199 0.35 -0.1 1,338,236 - PfGW456L13_1199 0.35 -0.1 1,338,236 - PfGW456L13_1199 0.35 +0.8 1,338,236 - PfGW456L13_1199 0.35 -0.2 1,338,236 - PfGW456L13_1199 0.35 +1.5 1,338,388 + PfGW456L13_1199 0.75 +0.1 1,338,388 + PfGW456L13_1199 0.75 -0.7 1,338,389 - PfGW456L13_1199 0.75 +0.8 1,338,389 - PfGW456L13_1199 0.75 -0.6 1,338,436 + PfGW456L13_1199 0.88 +0.7 1,338,495 - -0.1 1,338,519 + -1.2 1,338,519 + +0.5 1,338,520 - +1.5 1,338,520 - +0.1 1,338,520 - -0.5 1,338,520 - -0.8 1,338,538 + +1.0 1,338,547 + +0.4 1,338,554 + +0.9 1,338,557 + +0.0 1,338,557 + +0.3 1,338,557 + +0.3 1,338,558 - +0.3 1,338,558 - -0.6 1,338,558 - +1.9 1,339,071 - PfGW456L13_1200 0.58 +0.5
Or see this region's nucleotide sequence