Experiment: 1-ethyl-3-methylimidazolium acetate 30 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fecR and fecI overlap by 4 nucleotides fecI and insA-7 are separated by 291 nucleotides insA-7 and insB-7 overlap by 82 nucleotides
b4292: fecR - KpLE2 phage-like element; transmembrane signal transducer for ferric citrate transport (NCBI), at 4,514,787 to 4,515,740
fecR
b4293: fecI - KpLE2 phage-like element; RNA polymerase, sigma 19 factor (NCBI), at 4,515,737 to 4,516,258
fecI
b4294: insA-7 - KpLE2 phage-like element; IS1 repressor protein InsA (NCBI), at 4,516,550 to 4,516,825
insA-7
b4576: insB-7 - no description, at 4,516,744 to 4,517,247
insB-7
Position (kb) 4515
4516
4517 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4514.740 kb on - strand at 4514.962 kb on - strand, within fecR at 4515.006 kb on - strand, within fecR at 4515.017 kb on + strand, within fecR at 4515.044 kb on + strand, within fecR at 4515.059 kb on - strand, within fecR at 4515.152 kb on - strand, within fecR at 4515.299 kb on - strand, within fecR at 4515.303 kb on + strand, within fecR at 4515.311 kb on - strand, within fecR at 4515.504 kb on - strand, within fecR at 4515.504 kb on - strand, within fecR at 4515.522 kb on + strand, within fecR at 4515.546 kb on + strand, within fecR at 4515.584 kb on - strand, within fecR at 4515.609 kb on - strand, within fecR at 4515.647 kb on + strand at 4515.669 kb on + strand at 4515.669 kb on + strand at 4515.702 kb on - strand at 4515.749 kb on + strand at 4515.756 kb on - strand at 4515.760 kb on + strand at 4515.770 kb on + strand at 4515.852 kb on - strand, within fecI at 4515.928 kb on - strand, within fecI at 4515.928 kb on - strand, within fecI at 4515.997 kb on + strand, within fecI at 4516.053 kb on + strand, within fecI at 4516.053 kb on + strand, within fecI at 4516.059 kb on + strand, within fecI at 4516.059 kb on + strand, within fecI at 4516.067 kb on - strand, within fecI at 4516.067 kb on - strand, within fecI at 4516.111 kb on - strand, within fecI at 4516.116 kb on - strand, within fecI at 4516.135 kb on - strand, within fecI at 4516.377 kb on + strand at 4516.417 kb on + strand at 4516.430 kb on + strand at 4516.456 kb on + strand at 4516.515 kb on + strand at 4516.515 kb on + strand at 4516.686 kb on - strand, within insA-7 at 4516.686 kb on - strand, within insA-7 at 4516.884 kb on + strand, within insB-7 at 4516.946 kb on + strand, within insB-7 at 4516.959 kb on - strand, within insB-7 at 4516.983 kb on + strand, within insB-7 at 4516.983 kb on + strand, within insB-7 at 4517.038 kb on - strand, within insB-7 at 4517.038 kb on - strand, within insB-7 at 4517.064 kb on - strand, within insB-7 at 4517.088 kb on + strand, within insB-7 at 4517.100 kb on + strand, within insB-7 at 4517.108 kb on - strand, within insB-7 at 4517.146 kb on + strand, within insB-7 at 4517.146 kb on + strand, within insB-7
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 30 mM remove 4,514,740 - +1.4 4,514,962 - fecR b4292 0.18 -0.5 4,515,006 - fecR b4292 0.23 +0.6 4,515,017 + fecR b4292 0.24 +0.1 4,515,044 + fecR b4292 0.27 -0.2 4,515,059 - fecR b4292 0.29 -0.1 4,515,152 - fecR b4292 0.38 +0.7 4,515,299 - fecR b4292 0.54 -0.1 4,515,303 + fecR b4292 0.54 +0.4 4,515,311 - fecR b4292 0.55 -1.3 4,515,504 - fecR b4292 0.75 +0.1 4,515,504 - fecR b4292 0.75 -0.9 4,515,522 + fecR b4292 0.77 +1.1 4,515,546 + fecR b4292 0.80 -0.3 4,515,584 - fecR b4292 0.84 +0.8 4,515,609 - fecR b4292 0.86 +0.7 4,515,647 + -0.9 4,515,669 + +2.1 4,515,669 + +0.7 4,515,702 - +0.4 4,515,749 + -1.7 4,515,756 - +0.0 4,515,760 + +1.1 4,515,770 + -0.2 4,515,852 - fecI b4293 0.22 +1.0 4,515,928 - fecI b4293 0.37 +0.6 4,515,928 - fecI b4293 0.37 +1.4 4,515,997 + fecI b4293 0.50 -0.9 4,516,053 + fecI b4293 0.61 +1.1 4,516,053 + fecI b4293 0.61 -1.4 4,516,059 + fecI b4293 0.62 +1.0 4,516,059 + fecI b4293 0.62 -0.1 4,516,067 - fecI b4293 0.63 +0.2 4,516,067 - fecI b4293 0.63 +1.0 4,516,111 - fecI b4293 0.72 +0.9 4,516,116 - fecI b4293 0.73 +1.7 4,516,135 - fecI b4293 0.76 +0.2 4,516,377 + +0.9 4,516,417 + +0.2 4,516,430 + +0.7 4,516,456 + -0.1 4,516,515 + -0.7 4,516,515 + -0.1 4,516,686 - insA-7 b4294 0.49 +0.3 4,516,686 - insA-7 b4294 0.49 -1.9 4,516,884 + insB-7 b4576 0.28 +0.3 4,516,946 + insB-7 b4576 0.40 +0.3 4,516,959 - insB-7 b4576 0.43 +0.1 4,516,983 + insB-7 b4576 0.47 -0.0 4,516,983 + insB-7 b4576 0.47 -0.4 4,517,038 - insB-7 b4576 0.58 +0.1 4,517,038 - insB-7 b4576 0.58 -0.4 4,517,064 - insB-7 b4576 0.63 +1.1 4,517,088 + insB-7 b4576 0.68 -0.5 4,517,100 + insB-7 b4576 0.71 +0.3 4,517,108 - insB-7 b4576 0.72 -0.7 4,517,146 + insB-7 b4576 0.80 +0.9 4,517,146 + insB-7 b4576 0.80 +2.1
Or see this region's nucleotide sequence