Experiment: 1-ethyl-3-methylimidazolium acetate 30 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sgaT and ulaB are separated by 15 nucleotides ulaB and ulaC are separated by 9 nucleotides ulaC and ulaD are separated by 13 nucleotides
b4193: sgaT - orf, hypothetical protein (VIMSS), at 4,418,003 to 4,419,400
sgaT
b4194: ulaB - L-ascorbate-specific enzyme IIB component of PTS (NCBI), at 4,419,416 to 4,419,721
ulaB
b4195: ulaC - L-ascorbate-specific enzyme IIA component of PTS (NCBI), at 4,419,731 to 4,420,195
ulaC
b4196: ulaD - 3-keto-L-gulonate 6-phosphate decarboxylase (NCBI), at 4,420,209 to 4,420,859
ulaD
Position (kb)
4419
4420 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4418.474 kb on + strand, within sgaT at 4418.475 kb on + strand, within sgaT at 4418.579 kb on + strand, within sgaT at 4418.649 kb on + strand, within sgaT at 4418.692 kb on + strand, within sgaT at 4418.692 kb on + strand, within sgaT at 4418.736 kb on + strand, within sgaT at 4418.776 kb on - strand, within sgaT at 4418.787 kb on - strand, within sgaT at 4418.787 kb on - strand, within sgaT at 4418.798 kb on + strand, within sgaT at 4418.801 kb on + strand, within sgaT at 4418.801 kb on + strand, within sgaT at 4418.801 kb on + strand, within sgaT at 4418.801 kb on + strand, within sgaT at 4418.809 kb on - strand, within sgaT at 4418.809 kb on - strand, within sgaT at 4418.882 kb on - strand, within sgaT at 4418.914 kb on + strand, within sgaT at 4418.946 kb on + strand, within sgaT at 4419.069 kb on + strand, within sgaT at 4419.069 kb on + strand, within sgaT at 4419.148 kb on - strand, within sgaT at 4419.170 kb on + strand, within sgaT at 4419.245 kb on + strand, within sgaT at 4419.291 kb on + strand at 4419.357 kb on + strand at 4419.357 kb on + strand at 4419.431 kb on - strand at 4419.457 kb on - strand, within ulaB at 4419.584 kb on + strand, within ulaB at 4419.584 kb on + strand, within ulaB at 4419.584 kb on + strand, within ulaB at 4419.630 kb on + strand, within ulaB at 4419.869 kb on + strand, within ulaC at 4419.869 kb on + strand, within ulaC at 4419.924 kb on - strand, within ulaC at 4419.929 kb on - strand, within ulaC at 4419.947 kb on - strand, within ulaC at 4420.137 kb on + strand, within ulaC at 4420.164 kb on + strand at 4420.164 kb on + strand at 4420.206 kb on + strand at 4420.355 kb on - strand, within ulaD at 4420.363 kb on + strand, within ulaD at 4420.386 kb on - strand, within ulaD at 4420.479 kb on + strand, within ulaD at 4420.533 kb on + strand, within ulaD at 4420.641 kb on + strand, within ulaD at 4420.719 kb on + strand, within ulaD at 4420.719 kb on + strand, within ulaD
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 30 mM remove 4,418,474 + sgaT b4193 0.34 +0.3 4,418,475 + sgaT b4193 0.34 +0.2 4,418,579 + sgaT b4193 0.41 +0.5 4,418,649 + sgaT b4193 0.46 +0.2 4,418,692 + sgaT b4193 0.49 -0.3 4,418,692 + sgaT b4193 0.49 +0.6 4,418,736 + sgaT b4193 0.52 +0.7 4,418,776 - sgaT b4193 0.55 +0.6 4,418,787 - sgaT b4193 0.56 -2.3 4,418,787 - sgaT b4193 0.56 +1.6 4,418,798 + sgaT b4193 0.57 +0.4 4,418,801 + sgaT b4193 0.57 +0.7 4,418,801 + sgaT b4193 0.57 +0.2 4,418,801 + sgaT b4193 0.57 +0.4 4,418,801 + sgaT b4193 0.57 -0.1 4,418,809 - sgaT b4193 0.58 -0.1 4,418,809 - sgaT b4193 0.58 +0.5 4,418,882 - sgaT b4193 0.63 -1.1 4,418,914 + sgaT b4193 0.65 +0.6 4,418,946 + sgaT b4193 0.67 +1.5 4,419,069 + sgaT b4193 0.76 +0.8 4,419,069 + sgaT b4193 0.76 +0.4 4,419,148 - sgaT b4193 0.82 +1.5 4,419,170 + sgaT b4193 0.83 -0.2 4,419,245 + sgaT b4193 0.89 -0.7 4,419,291 + -0.6 4,419,357 + +0.2 4,419,357 + -0.4 4,419,431 - -1.0 4,419,457 - ulaB b4194 0.13 +1.2 4,419,584 + ulaB b4194 0.55 +1.3 4,419,584 + ulaB b4194 0.55 +2.2 4,419,584 + ulaB b4194 0.55 +1.4 4,419,630 + ulaB b4194 0.70 -0.3 4,419,869 + ulaC b4195 0.30 -1.0 4,419,869 + ulaC b4195 0.30 -0.3 4,419,924 - ulaC b4195 0.42 +1.2 4,419,929 - ulaC b4195 0.43 +0.2 4,419,947 - ulaC b4195 0.46 +0.8 4,420,137 + ulaC b4195 0.87 +1.2 4,420,164 + +3.2 4,420,164 + +0.7 4,420,206 + +0.8 4,420,355 - ulaD b4196 0.22 +1.2 4,420,363 + ulaD b4196 0.24 +2.2 4,420,386 - ulaD b4196 0.27 +0.9 4,420,479 + ulaD b4196 0.41 +0.3 4,420,533 + ulaD b4196 0.50 +0.4 4,420,641 + ulaD b4196 0.66 -0.8 4,420,719 + ulaD b4196 0.78 +0.1 4,420,719 + ulaD b4196 0.78 +0.8
Or see this region's nucleotide sequence