Strain Fitness in Escherichia coli BW25113 around b3951

Experiment: 1-ethyl-3-methylimidazolium acetate 30 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfrwC and frwB are separated by 14 nucleotidesfrwB and pflD are separated by 50 nucleotidespflD and pflC overlap by 35 nucleotidespflC and frwD are separated by 1 nucleotides b3949: frwC - predicted enzyme IIC component of PTS (NCBI), at 4,140,553 to 4,141,632 frwC b3950: frwB - predicted enzyme IIB component of PTS (NCBI), at 4,141,647 to 4,141,967 frwB b3951: pflD - predicted formate acetyltransferase 2 (pyruvate formate lyase II) (NCBI), at 4,142,018 to 4,144,315 pflD b3952: pflC - probable pyruvate formate lyase activating enzyme 2 (VIMSS), at 4,144,281 to 4,145,159 pflC b3953: frwD - predicted enzyme IIB component of PTS (NCBI), at 4,145,161 to 4,145,502 frwD Position (kb) 4142 4143 4144 4145Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4141.051 kb on - strand, within frwCat 4141.066 kb on + strand, within frwCat 4141.069 kb on + strand, within frwCat 4141.069 kb on + strand, within frwCat 4141.069 kb on + strand, within frwCat 4141.092 kb on - strand, within frwCat 4141.105 kb on + strand, within frwCat 4141.130 kb on - strand, within frwCat 4141.133 kb on + strand, within frwCat 4141.154 kb on + strand, within frwCat 4141.167 kb on + strand, within frwCat 4141.191 kb on - strand, within frwCat 4141.194 kb on - strand, within frwCat 4141.194 kb on - strand, within frwCat 4141.218 kb on + strand, within frwCat 4141.218 kb on + strand, within frwCat 4141.255 kb on + strand, within frwCat 4141.255 kb on + strand, within frwCat 4141.311 kb on + strand, within frwCat 4141.311 kb on + strand, within frwCat 4141.320 kb on - strand, within frwCat 4141.320 kb on - strand, within frwCat 4141.320 kb on - strand, within frwCat 4141.441 kb on - strand, within frwCat 4141.448 kb on - strand, within frwCat 4141.448 kb on - strand, within frwCat 4141.449 kb on + strand, within frwCat 4141.450 kb on + strand, within frwCat 4141.494 kb on + strand, within frwCat 4141.549 kb on - strandat 4141.549 kb on - strandat 4141.676 kb on + strandat 4141.692 kb on - strand, within frwBat 4141.692 kb on - strand, within frwBat 4141.816 kb on + strand, within frwBat 4141.832 kb on + strand, within frwBat 4141.832 kb on + strand, within frwBat 4141.969 kb on + strandat 4142.009 kb on + strandat 4142.028 kb on + strandat 4142.092 kb on + strandat 4142.204 kb on - strandat 4142.223 kb on + strandat 4142.225 kb on + strandat 4142.282 kb on + strand, within pflDat 4142.282 kb on + strand, within pflDat 4142.357 kb on - strand, within pflDat 4142.357 kb on - strand, within pflDat 4142.370 kb on - strand, within pflDat 4142.370 kb on - strand, within pflDat 4142.410 kb on + strand, within pflDat 4142.461 kb on + strand, within pflDat 4142.461 kb on + strand, within pflDat 4142.476 kb on - strand, within pflDat 4142.476 kb on - strand, within pflDat 4142.497 kb on - strand, within pflDat 4142.497 kb on - strand, within pflDat 4142.499 kb on + strand, within pflDat 4142.528 kb on - strand, within pflDat 4142.602 kb on + strand, within pflDat 4142.672 kb on + strand, within pflDat 4142.680 kb on + strand, within pflDat 4142.683 kb on - strand, within pflDat 4142.683 kb on - strand, within pflDat 4142.688 kb on - strand, within pflDat 4142.690 kb on + strand, within pflDat 4142.690 kb on + strand, within pflDat 4142.698 kb on + strand, within pflDat 4142.705 kb on + strand, within pflDat 4142.736 kb on + strand, within pflDat 4142.753 kb on - strand, within pflDat 4142.765 kb on - strand, within pflDat 4142.781 kb on + strand, within pflDat 4142.834 kb on + strand, within pflDat 4142.834 kb on + strand, within pflDat 4142.837 kb on + strand, within pflDat 4142.862 kb on - strand, within pflDat 4142.862 kb on - strand, within pflDat 4142.865 kb on + strand, within pflDat 4142.870 kb on + strand, within pflDat 4142.873 kb on - strand, within pflDat 4142.905 kb on + strand, within pflDat 4142.905 kb on + strand, within pflDat 4142.905 kb on + strand, within pflDat 4142.934 kb on + strand, within pflDat 4142.942 kb on - strand, within pflDat 4142.944 kb on + strand, within pflDat 4142.944 kb on + strand, within pflDat 4142.989 kb on + strand, within pflDat 4142.994 kb on + strand, within pflDat 4142.994 kb on + strand, within pflDat 4142.994 kb on + strand, within pflDat 4142.994 kb on + strand, within pflDat 4143.035 kb on - strand, within pflDat 4143.053 kb on - strand, within pflDat 4143.070 kb on + strand, within pflDat 4143.126 kb on - strand, within pflDat 4143.220 kb on + strand, within pflDat 4143.220 kb on + strand, within pflDat 4143.258 kb on + strand, within pflDat 4143.264 kb on - strand, within pflDat 4143.270 kb on + strand, within pflDat 4143.270 kb on + strand, within pflDat 4143.434 kb on + strand, within pflDat 4143.436 kb on - strand, within pflDat 4143.445 kb on - strand, within pflDat 4143.445 kb on - strand, within pflDat 4143.446 kb on + strand, within pflDat 4143.718 kb on + strand, within pflDat 4143.718 kb on + strand, within pflDat 4143.776 kb on + strand, within pflDat 4143.784 kb on - strand, within pflDat 4143.802 kb on - strand, within pflDat 4143.802 kb on - strand, within pflDat 4143.853 kb on - strand, within pflDat 4143.862 kb on + strand, within pflDat 4143.862 kb on + strand, within pflDat 4143.870 kb on + strand, within pflDat 4143.870 kb on + strand, within pflDat 4143.872 kb on + strand, within pflDat 4143.872 kb on - strand, within pflDat 4143.872 kb on - strand, within pflDat 4143.883 kb on + strand, within pflDat 4143.895 kb on - strand, within pflDat 4144.012 kb on + strand, within pflDat 4144.012 kb on + strand, within pflDat 4144.019 kb on + strand, within pflDat 4144.020 kb on - strand, within pflDat 4144.021 kb on + strand, within pflDat 4144.036 kb on + strand, within pflDat 4144.039 kb on - strand, within pflDat 4144.066 kb on - strand, within pflDat 4144.079 kb on + strand, within pflDat 4144.084 kb on - strand, within pflDat 4144.086 kb on + strandat 4144.133 kb on - strandat 4144.135 kb on + strandat 4144.135 kb on + strandat 4144.162 kb on - strandat 4144.235 kb on + strandat 4144.235 kb on + strandat 4144.243 kb on - strandat 4144.243 kb on - strandat 4144.287 kb on - strandat 4144.304 kb on - strandat 4144.317 kb on - strandat 4144.317 kb on - strandat 4144.409 kb on + strand, within pflCat 4144.445 kb on - strand, within pflCat 4144.454 kb on + strand, within pflCat 4144.454 kb on + strand, within pflCat 4144.479 kb on + strand, within pflCat 4144.479 kb on + strand, within pflCat 4144.505 kb on - strand, within pflCat 4144.592 kb on - strand, within pflCat 4144.592 kb on - strand, within pflCat 4144.599 kb on + strand, within pflCat 4144.599 kb on + strand, within pflCat 4144.709 kb on + strand, within pflCat 4144.770 kb on - strand, within pflCat 4144.841 kb on + strand, within pflCat 4144.952 kb on + strand, within pflCat 4144.983 kb on - strand, within pflCat 4144.983 kb on - strand, within pflCat 4145.095 kb on - strandat 4145.097 kb on + strandat 4145.097 kb on + strandat 4145.109 kb on - strandat 4145.142 kb on - strandat 4145.142 kb on - strandat 4145.142 kb on - strandat 4145.190 kb on + strandat 4145.190 kb on + strandat 4145.261 kb on - strand, within frwDat 4145.263 kb on - strand, within frwDat 4145.264 kb on - strand, within frwD

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 30 mM
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4,141,051 - frwC b3949 0.46 +0.4
4,141,066 + frwC b3949 0.47 +0.2
4,141,069 + frwC b3949 0.48 -0.8
4,141,069 + frwC b3949 0.48 +0.5
4,141,069 + frwC b3949 0.48 -1.4
4,141,092 - frwC b3949 0.50 -0.0
4,141,105 + frwC b3949 0.51 -0.9
4,141,130 - frwC b3949 0.53 +0.7
4,141,133 + frwC b3949 0.54 -1.5
4,141,154 + frwC b3949 0.56 -0.7
4,141,167 + frwC b3949 0.57 +1.6
4,141,191 - frwC b3949 0.59 +0.7
4,141,194 - frwC b3949 0.59 +0.2
4,141,194 - frwC b3949 0.59 +1.4
4,141,218 + frwC b3949 0.62 -0.1
4,141,218 + frwC b3949 0.62 +0.4
4,141,255 + frwC b3949 0.65 +0.7
4,141,255 + frwC b3949 0.65 +0.4
4,141,311 + frwC b3949 0.70 +0.6
4,141,311 + frwC b3949 0.70 +0.1
4,141,320 - frwC b3949 0.71 +0.1
4,141,320 - frwC b3949 0.71 +0.1
4,141,320 - frwC b3949 0.71 +2.2
4,141,441 - frwC b3949 0.82 +0.7
4,141,448 - frwC b3949 0.83 +0.1
4,141,448 - frwC b3949 0.83 -0.0
4,141,449 + frwC b3949 0.83 +0.7
4,141,450 + frwC b3949 0.83 +0.6
4,141,494 + frwC b3949 0.87 -1.8
4,141,549 - +1.7
4,141,549 - +0.6
4,141,676 + +0.4
4,141,692 - frwB b3950 0.14 -0.6
4,141,692 - frwB b3950 0.14 -0.9
4,141,816 + frwB b3950 0.53 -0.8
4,141,832 + frwB b3950 0.58 -1.8
4,141,832 + frwB b3950 0.58 +0.6
4,141,969 + +0.9
4,142,009 + +0.7
4,142,028 + +0.0
4,142,092 + -0.4
4,142,204 - -0.4
4,142,223 + -0.4
4,142,225 + +0.6
4,142,282 + pflD b3951 0.11 +0.1
4,142,282 + pflD b3951 0.11 -0.5
4,142,357 - pflD b3951 0.15 +1.1
4,142,357 - pflD b3951 0.15 +0.4
4,142,370 - pflD b3951 0.15 +1.8
4,142,370 - pflD b3951 0.15 +1.6
4,142,410 + pflD b3951 0.17 +2.4
4,142,461 + pflD b3951 0.19 +0.6
4,142,461 + pflD b3951 0.19 -1.0
4,142,476 - pflD b3951 0.20 -1.2
4,142,476 - pflD b3951 0.20 -0.3
4,142,497 - pflD b3951 0.21 -0.1
4,142,497 - pflD b3951 0.21 -0.1
4,142,499 + pflD b3951 0.21 +0.8
4,142,528 - pflD b3951 0.22 +0.1
4,142,602 + pflD b3951 0.25 -0.1
4,142,672 + pflD b3951 0.28 +1.5
4,142,680 + pflD b3951 0.29 +0.2
4,142,683 - pflD b3951 0.29 +1.4
4,142,683 - pflD b3951 0.29 +1.0
4,142,688 - pflD b3951 0.29 +1.6
4,142,690 + pflD b3951 0.29 -1.2
4,142,690 + pflD b3951 0.29 +0.1
4,142,698 + pflD b3951 0.30 +0.4
4,142,705 + pflD b3951 0.30 +1.1
4,142,736 + pflD b3951 0.31 +2.1
4,142,753 - pflD b3951 0.32 -1.2
4,142,765 - pflD b3951 0.33 -0.6
4,142,781 + pflD b3951 0.33 -0.2
4,142,834 + pflD b3951 0.36 +1.8
4,142,834 + pflD b3951 0.36 +0.8
4,142,837 + pflD b3951 0.36 +1.4
4,142,862 - pflD b3951 0.37 -1.0
4,142,862 - pflD b3951 0.37 -0.8
4,142,865 + pflD b3951 0.37 +0.4
4,142,870 + pflD b3951 0.37 +0.4
4,142,873 - pflD b3951 0.37 -1.1
4,142,905 + pflD b3951 0.39 -1.2
4,142,905 + pflD b3951 0.39 -1.8
4,142,905 + pflD b3951 0.39 -0.2
4,142,934 + pflD b3951 0.40 +0.3
4,142,942 - pflD b3951 0.40 +0.1
4,142,944 + pflD b3951 0.40 +0.6
4,142,944 + pflD b3951 0.40 +0.8
4,142,989 + pflD b3951 0.42 +0.2
4,142,994 + pflD b3951 0.42 -0.2
4,142,994 + pflD b3951 0.42 +0.2
4,142,994 + pflD b3951 0.42 +0.4
4,142,994 + pflD b3951 0.42 +0.4
4,143,035 - pflD b3951 0.44 -1.1
4,143,053 - pflD b3951 0.45 +0.0
4,143,070 + pflD b3951 0.46 +0.7
4,143,126 - pflD b3951 0.48 +1.9
4,143,220 + pflD b3951 0.52 +1.1
4,143,220 + pflD b3951 0.52 +0.8
4,143,258 + pflD b3951 0.54 -0.2
4,143,264 - pflD b3951 0.54 +0.6
4,143,270 + pflD b3951 0.54 -0.6
4,143,270 + pflD b3951 0.54 +0.4
4,143,434 + pflD b3951 0.62 +0.1
4,143,436 - pflD b3951 0.62 +0.1
4,143,445 - pflD b3951 0.62 +1.2
4,143,445 - pflD b3951 0.62 +0.4
4,143,446 + pflD b3951 0.62 +0.6
4,143,718 + pflD b3951 0.74 +0.3
4,143,718 + pflD b3951 0.74 +0.0
4,143,776 + pflD b3951 0.77 -0.5
4,143,784 - pflD b3951 0.77 +0.5
4,143,802 - pflD b3951 0.78 +0.0
4,143,802 - pflD b3951 0.78 +0.4
4,143,853 - pflD b3951 0.80 +0.2
4,143,862 + pflD b3951 0.80 +0.0
4,143,862 + pflD b3951 0.80 +0.6
4,143,870 + pflD b3951 0.81 -1.0
4,143,870 + pflD b3951 0.81 +1.4
4,143,872 + pflD b3951 0.81 +0.1
4,143,872 - pflD b3951 0.81 +0.0
4,143,872 - pflD b3951 0.81 +1.4
4,143,883 + pflD b3951 0.81 +0.2
4,143,895 - pflD b3951 0.82 +0.8
4,144,012 + pflD b3951 0.87 -1.1
4,144,012 + pflD b3951 0.87 -1.6
4,144,019 + pflD b3951 0.87 +1.1
4,144,020 - pflD b3951 0.87 -0.1
4,144,021 + pflD b3951 0.87 +0.2
4,144,036 + pflD b3951 0.88 -0.4
4,144,039 - pflD b3951 0.88 +0.6
4,144,066 - pflD b3951 0.89 +0.4
4,144,079 + pflD b3951 0.90 -0.2
4,144,084 - pflD b3951 0.90 -0.6
4,144,086 + +0.4
4,144,133 - +1.2
4,144,135 + +0.8
4,144,135 + -0.1
4,144,162 - +0.1
4,144,235 + +0.7
4,144,235 + -0.4
4,144,243 - +1.1
4,144,243 - +0.7
4,144,287 - -2.0
4,144,304 - -1.3
4,144,317 - +0.3
4,144,317 - -1.4
4,144,409 + pflC b3952 0.15 +0.9
4,144,445 - pflC b3952 0.19 +0.2
4,144,454 + pflC b3952 0.20 +0.9
4,144,454 + pflC b3952 0.20 +0.7
4,144,479 + pflC b3952 0.23 -0.2
4,144,479 + pflC b3952 0.23 +0.6
4,144,505 - pflC b3952 0.25 +0.6
4,144,592 - pflC b3952 0.35 -0.1
4,144,592 - pflC b3952 0.35 -0.5
4,144,599 + pflC b3952 0.36 -0.0
4,144,599 + pflC b3952 0.36 -0.2
4,144,709 + pflC b3952 0.49 -0.0
4,144,770 - pflC b3952 0.56 -1.4
4,144,841 + pflC b3952 0.64 +0.5
4,144,952 + pflC b3952 0.76 +1.0
4,144,983 - pflC b3952 0.80 +2.8
4,144,983 - pflC b3952 0.80 +0.0
4,145,095 - +1.4
4,145,097 + +0.8
4,145,097 + +0.0
4,145,109 - +0.8
4,145,142 - -1.4
4,145,142 - +0.0
4,145,142 - +1.1
4,145,190 + +0.6
4,145,190 + -0.6
4,145,261 - frwD b3953 0.29 +0.4
4,145,263 - frwD b3953 0.30 -0.2
4,145,264 - frwD b3953 0.30 +0.4

Or see this region's nucleotide sequence