Experiment: 1-ethyl-3-methylimidazolium acetate 30 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fdhE and fdoI overlap by 4 nucleotides fdoI and fdoH overlap by 4 nucleotides fdoH and fdoG are separated by 12 nucleotides
b3891: fdhE - formate dehydrogenase accessory protein FdhE (NCBI), at 4,078,322 to 4,079,251
fdhE
b3892: fdoI - formate dehydrogenase-O, cytochrome b556 subunit (NCBI), at 4,079,248 to 4,079,883
fdoI
b3893: fdoH - formate dehydrogenase-O, Fe-S subunit (NCBI), at 4,079,880 to 4,080,782
fdoH
b3894: fdoG - formate dehydrogenase-O, large subunit (NCBI), at 4,080,795 to 4,083,845
fdoG
Position (kb)
4079
4080 Strain fitness (log2 ratio)
-1
0
1
2
3 at 4078.262 kb on + strand at 4078.262 kb on + strand at 4078.264 kb on + strand at 4078.264 kb on + strand at 4078.271 kb on + strand at 4078.277 kb on - strand at 4078.280 kb on - strand at 4078.294 kb on - strand at 4078.294 kb on - strand at 4078.371 kb on + strand at 4078.521 kb on - strand, within fdhE at 4078.521 kb on - strand, within fdhE at 4078.532 kb on + strand, within fdhE at 4078.532 kb on + strand, within fdhE at 4078.601 kb on + strand, within fdhE at 4078.618 kb on + strand, within fdhE at 4078.618 kb on - strand, within fdhE at 4078.619 kb on + strand, within fdhE at 4078.619 kb on + strand, within fdhE at 4078.622 kb on - strand, within fdhE at 4078.622 kb on - strand, within fdhE at 4078.635 kb on - strand, within fdhE at 4078.635 kb on - strand, within fdhE at 4078.639 kb on + strand, within fdhE at 4078.639 kb on + strand, within fdhE at 4078.650 kb on + strand, within fdhE at 4078.650 kb on + strand, within fdhE at 4078.650 kb on + strand, within fdhE at 4078.650 kb on + strand, within fdhE at 4078.650 kb on + strand, within fdhE at 4078.650 kb on + strand, within fdhE at 4078.650 kb on + strand, within fdhE at 4078.650 kb on + strand, within fdhE at 4078.658 kb on - strand, within fdhE at 4078.727 kb on + strand, within fdhE at 4078.727 kb on + strand, within fdhE at 4078.740 kb on + strand, within fdhE at 4078.749 kb on - strand, within fdhE at 4078.749 kb on - strand, within fdhE at 4078.770 kb on - strand, within fdhE at 4078.770 kb on - strand, within fdhE at 4078.814 kb on + strand, within fdhE at 4078.838 kb on + strand, within fdhE at 4078.981 kb on - strand, within fdhE at 4078.981 kb on - strand, within fdhE at 4078.991 kb on - strand, within fdhE at 4079.019 kb on + strand, within fdhE at 4079.038 kb on - strand, within fdhE at 4079.042 kb on + strand, within fdhE at 4079.058 kb on + strand, within fdhE at 4079.064 kb on - strand, within fdhE at 4079.092 kb on + strand, within fdhE at 4079.120 kb on - strand, within fdhE at 4079.195 kb on - strand at 4079.195 kb on - strand at 4079.195 kb on - strand at 4079.198 kb on - strand at 4079.280 kb on + strand at 4079.291 kb on - strand at 4079.295 kb on - strand at 4079.295 kb on - strand at 4079.309 kb on - strand at 4079.325 kb on + strand, within fdoI at 4079.325 kb on - strand, within fdoI at 4079.325 kb on - strand, within fdoI at 4079.340 kb on - strand, within fdoI at 4079.343 kb on - strand, within fdoI at 4079.380 kb on + strand, within fdoI at 4079.380 kb on + strand, within fdoI at 4079.380 kb on + strand, within fdoI at 4079.388 kb on - strand, within fdoI at 4079.388 kb on - strand, within fdoI at 4079.438 kb on - strand, within fdoI at 4079.581 kb on - strand, within fdoI at 4079.581 kb on - strand, within fdoI at 4079.581 kb on - strand, within fdoI at 4079.581 kb on - strand, within fdoI at 4079.586 kb on - strand, within fdoI at 4079.626 kb on + strand, within fdoI at 4079.654 kb on - strand, within fdoI at 4079.654 kb on - strand, within fdoI at 4079.665 kb on - strand, within fdoI at 4079.665 kb on - strand, within fdoI at 4079.680 kb on - strand, within fdoI at 4079.689 kb on + strand, within fdoI at 4079.741 kb on + strand, within fdoI at 4079.761 kb on + strand, within fdoI at 4079.766 kb on - strand, within fdoI at 4079.769 kb on - strand, within fdoI at 4079.769 kb on - strand, within fdoI at 4079.818 kb on - strand, within fdoI at 4079.832 kb on - strand at 4079.903 kb on - strand at 4079.903 kb on - strand at 4079.930 kb on - strand at 4079.930 kb on - strand at 4079.937 kb on + strand at 4079.945 kb on - strand at 4079.945 kb on - strand at 4079.945 kb on - strand at 4079.945 kb on - strand at 4079.960 kb on - strand at 4079.965 kb on + strand at 4079.999 kb on - strand, within fdoH at 4079.999 kb on - strand, within fdoH at 4080.077 kb on - strand, within fdoH at 4080.096 kb on + strand, within fdoH at 4080.096 kb on + strand, within fdoH at 4080.096 kb on + strand, within fdoH at 4080.104 kb on + strand, within fdoH at 4080.104 kb on - strand, within fdoH at 4080.105 kb on - strand, within fdoH at 4080.121 kb on - strand, within fdoH at 4080.138 kb on + strand, within fdoH at 4080.141 kb on + strand, within fdoH at 4080.165 kb on - strand, within fdoH at 4080.169 kb on + strand, within fdoH at 4080.278 kb on + strand, within fdoH at 4080.288 kb on + strand, within fdoH at 4080.299 kb on + strand, within fdoH at 4080.299 kb on + strand, within fdoH at 4080.345 kb on + strand, within fdoH at 4080.394 kb on + strand, within fdoH at 4080.395 kb on - strand, within fdoH at 4080.410 kb on - strand, within fdoH at 4080.415 kb on + strand, within fdoH at 4080.415 kb on + strand, within fdoH at 4080.460 kb on - strand, within fdoH at 4080.460 kb on - strand, within fdoH at 4080.462 kb on + strand, within fdoH at 4080.480 kb on - strand, within fdoH at 4080.486 kb on - strand, within fdoH at 4080.486 kb on - strand, within fdoH at 4080.540 kb on + strand, within fdoH at 4080.548 kb on - strand, within fdoH at 4080.571 kb on + strand, within fdoH at 4080.582 kb on + strand, within fdoH at 4080.582 kb on + strand, within fdoH at 4080.598 kb on - strand, within fdoH at 4080.598 kb on - strand, within fdoH at 4080.598 kb on - strand, within fdoH at 4080.608 kb on + strand, within fdoH at 4080.632 kb on - strand, within fdoH at 4080.649 kb on - strand, within fdoH at 4080.653 kb on + strand, within fdoH at 4080.653 kb on + strand, within fdoH at 4080.670 kb on + strand, within fdoH at 4080.670 kb on + strand, within fdoH at 4080.707 kb on - strand at 4080.707 kb on - strand at 4080.786 kb on - strand at 4080.786 kb on - strand at 4080.792 kb on + strand at 4080.831 kb on - strand at 4080.842 kb on + strand at 4080.864 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 30 mM remove 4,078,262 + -1.6 4,078,262 + +0.4 4,078,264 + -0.2 4,078,264 + -0.0 4,078,271 + +0.4 4,078,277 - -0.4 4,078,280 - -0.4 4,078,294 - +2.0 4,078,294 - +1.4 4,078,371 + +2.0 4,078,521 - fdhE b3891 0.21 +1.4 4,078,521 - fdhE b3891 0.21 +0.6 4,078,532 + fdhE b3891 0.23 +0.2 4,078,532 + fdhE b3891 0.23 -0.2 4,078,601 + fdhE b3891 0.30 +1.1 4,078,618 + fdhE b3891 0.32 +0.6 4,078,618 - fdhE b3891 0.32 +0.1 4,078,619 + fdhE b3891 0.32 +0.4 4,078,619 + fdhE b3891 0.32 +2.4 4,078,622 - fdhE b3891 0.32 +1.4 4,078,622 - fdhE b3891 0.32 +1.1 4,078,635 - fdhE b3891 0.34 +0.9 4,078,635 - fdhE b3891 0.34 +0.7 4,078,639 + fdhE b3891 0.34 +2.4 4,078,639 + fdhE b3891 0.34 +1.0 4,078,650 + fdhE b3891 0.35 -0.2 4,078,650 + fdhE b3891 0.35 +1.0 4,078,650 + fdhE b3891 0.35 +0.3 4,078,650 + fdhE b3891 0.35 +0.4 4,078,650 + fdhE b3891 0.35 +0.4 4,078,650 + fdhE b3891 0.35 -0.1 4,078,650 + fdhE b3891 0.35 +1.1 4,078,650 + fdhE b3891 0.35 +0.5 4,078,658 - fdhE b3891 0.36 +1.4 4,078,727 + fdhE b3891 0.44 -0.2 4,078,727 + fdhE b3891 0.44 +2.0 4,078,740 + fdhE b3891 0.45 +0.4 4,078,749 - fdhE b3891 0.46 +0.4 4,078,749 - fdhE b3891 0.46 +0.4 4,078,770 - fdhE b3891 0.48 +0.5 4,078,770 - fdhE b3891 0.48 +0.5 4,078,814 + fdhE b3891 0.53 -0.2 4,078,838 + fdhE b3891 0.55 -0.3 4,078,981 - fdhE b3891 0.71 -0.2 4,078,981 - fdhE b3891 0.71 +0.4 4,078,991 - fdhE b3891 0.72 +1.7 4,079,019 + fdhE b3891 0.75 +0.1 4,079,038 - fdhE b3891 0.77 -0.2 4,079,042 + fdhE b3891 0.77 +1.7 4,079,058 + fdhE b3891 0.79 +0.4 4,079,064 - fdhE b3891 0.80 -0.2 4,079,092 + fdhE b3891 0.83 +0.2 4,079,120 - fdhE b3891 0.86 +0.3 4,079,195 - +2.4 4,079,195 - +1.4 4,079,195 - +1.2 4,079,198 - +0.2 4,079,280 + +0.2 4,079,291 - +0.6 4,079,295 - -1.3 4,079,295 - +1.7 4,079,309 - +0.6 4,079,325 + fdoI b3892 0.12 +0.3 4,079,325 - fdoI b3892 0.12 -0.1 4,079,325 - fdoI b3892 0.12 +1.8 4,079,340 - fdoI b3892 0.14 +0.6 4,079,343 - fdoI b3892 0.15 +1.9 4,079,380 + fdoI b3892 0.21 +0.2 4,079,380 + fdoI b3892 0.21 +0.7 4,079,380 + fdoI b3892 0.21 +0.8 4,079,388 - fdoI b3892 0.22 +1.4 4,079,388 - fdoI b3892 0.22 -0.9 4,079,438 - fdoI b3892 0.30 +1.1 4,079,581 - fdoI b3892 0.52 -1.4 4,079,581 - fdoI b3892 0.52 +1.3 4,079,581 - fdoI b3892 0.52 +0.5 4,079,581 - fdoI b3892 0.52 +1.7 4,079,586 - fdoI b3892 0.53 +1.7 4,079,626 + fdoI b3892 0.59 -0.1 4,079,654 - fdoI b3892 0.64 -0.5 4,079,654 - fdoI b3892 0.64 +0.8 4,079,665 - fdoI b3892 0.66 +2.4 4,079,665 - fdoI b3892 0.66 +0.5 4,079,680 - fdoI b3892 0.68 +1.4 4,079,689 + fdoI b3892 0.69 +1.2 4,079,741 + fdoI b3892 0.78 +0.4 4,079,761 + fdoI b3892 0.81 +1.2 4,079,766 - fdoI b3892 0.81 +0.2 4,079,769 - fdoI b3892 0.82 +0.7 4,079,769 - fdoI b3892 0.82 +1.9 4,079,818 - fdoI b3892 0.90 +1.4 4,079,832 - +1.4 4,079,903 - +0.7 4,079,903 - +0.6 4,079,930 - +0.5 4,079,930 - +0.7 4,079,937 + +0.2 4,079,945 - +1.9 4,079,945 - +3.4 4,079,945 - +1.2 4,079,945 - +0.9 4,079,960 - +0.2 4,079,965 + +0.5 4,079,999 - fdoH b3893 0.13 +2.0 4,079,999 - fdoH b3893 0.13 +0.7 4,080,077 - fdoH b3893 0.22 +0.1 4,080,096 + fdoH b3893 0.24 +0.6 4,080,096 + fdoH b3893 0.24 +0.2 4,080,096 + fdoH b3893 0.24 +1.2 4,080,104 + fdoH b3893 0.25 +1.4 4,080,104 - fdoH b3893 0.25 -0.6 4,080,105 - fdoH b3893 0.25 -1.0 4,080,121 - fdoH b3893 0.27 +0.1 4,080,138 + fdoH b3893 0.29 +0.5 4,080,141 + fdoH b3893 0.29 +1.2 4,080,165 - fdoH b3893 0.32 -1.1 4,080,169 + fdoH b3893 0.32 +3.4 4,080,278 + fdoH b3893 0.44 -0.2 4,080,288 + fdoH b3893 0.45 +0.9 4,080,299 + fdoH b3893 0.46 +0.4 4,080,299 + fdoH b3893 0.46 -0.5 4,080,345 + fdoH b3893 0.51 +0.7 4,080,394 + fdoH b3893 0.57 +0.8 4,080,395 - fdoH b3893 0.57 +0.8 4,080,410 - fdoH b3893 0.59 +1.4 4,080,415 + fdoH b3893 0.59 +1.4 4,080,415 + fdoH b3893 0.59 +1.4 4,080,460 - fdoH b3893 0.64 +1.4 4,080,460 - fdoH b3893 0.64 +1.7 4,080,462 + fdoH b3893 0.64 +0.4 4,080,480 - fdoH b3893 0.66 +1.0 4,080,486 - fdoH b3893 0.67 +1.1 4,080,486 - fdoH b3893 0.67 +0.8 4,080,540 + fdoH b3893 0.73 +0.6 4,080,548 - fdoH b3893 0.74 +1.6 4,080,571 + fdoH b3893 0.77 +0.1 4,080,582 + fdoH b3893 0.78 +0.2 4,080,582 + fdoH b3893 0.78 +0.2 4,080,598 - fdoH b3893 0.80 +1.4 4,080,598 - fdoH b3893 0.80 -0.4 4,080,598 - fdoH b3893 0.80 +0.0 4,080,608 + fdoH b3893 0.81 +0.7 4,080,632 - fdoH b3893 0.83 +1.0 4,080,649 - fdoH b3893 0.85 +0.2 4,080,653 + fdoH b3893 0.86 -0.6 4,080,653 + fdoH b3893 0.86 +0.1 4,080,670 + fdoH b3893 0.87 +0.4 4,080,670 + fdoH b3893 0.87 -1.4 4,080,707 - -0.3 4,080,707 - +1.8 4,080,786 - +1.4 4,080,786 - +0.1 4,080,792 + +0.9 4,080,831 - +1.4 4,080,842 + +0.8 4,080,864 - +0.7
Or see this region's nucleotide sequence