Experiment: 1-ethyl-3-methylimidazolium acetate 30 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hemY and hemX are separated by 2 nucleotides hemX and hemD are separated by 21 nucleotides hemD and hemC overlap by 4 nucleotides
b3802: hemY - predicted protoheme IX synthesis protein (NCBI), at 3,984,709 to 3,985,905
hemY
b3803: hemX - predicted uroporphyrinogen III methylase (NCBI), at 3,985,908 to 3,987,089
hemX
b3804: hemD - uroporphyrinogen-III synthetase (NCBI), at 3,987,111 to 3,987,851
hemD
b3805: hemC - porphobilinogen deaminase (NCBI), at 3,987,848 to 3,988,789
hemC
Position (kb)
3985
3986
3987
3988 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3984.908 kb on - strand, within hemY at 3984.908 kb on - strand, within hemY at 3984.911 kb on + strand, within hemY at 3984.911 kb on + strand, within hemY at 3984.914 kb on - strand, within hemY at 3984.914 kb on - strand, within hemY at 3984.926 kb on - strand, within hemY at 3984.926 kb on - strand, within hemY at 3985.008 kb on + strand, within hemY at 3985.041 kb on - strand, within hemY at 3985.083 kb on + strand, within hemY at 3985.115 kb on + strand, within hemY at 3985.115 kb on - strand, within hemY at 3985.118 kb on + strand, within hemY at 3985.120 kb on + strand, within hemY at 3985.121 kb on - strand, within hemY at 3985.121 kb on - strand, within hemY at 3985.121 kb on - strand, within hemY at 3985.146 kb on - strand, within hemY at 3985.174 kb on + strand, within hemY at 3985.177 kb on - strand, within hemY at 3985.189 kb on + strand, within hemY at 3985.189 kb on + strand, within hemY at 3985.208 kb on + strand, within hemY at 3985.208 kb on + strand, within hemY at 3985.211 kb on - strand, within hemY at 3985.230 kb on - strand, within hemY at 3985.230 kb on - strand, within hemY at 3985.230 kb on - strand, within hemY at 3985.255 kb on - strand, within hemY at 3985.255 kb on - strand, within hemY at 3985.260 kb on - strand, within hemY at 3985.273 kb on + strand, within hemY at 3985.287 kb on + strand, within hemY at 3985.302 kb on - strand, within hemY at 3985.305 kb on - strand, within hemY at 3985.305 kb on - strand, within hemY at 3985.315 kb on - strand, within hemY at 3985.321 kb on - strand, within hemY at 3985.321 kb on - strand, within hemY at 3985.321 kb on - strand, within hemY at 3985.334 kb on - strand, within hemY at 3985.334 kb on - strand, within hemY at 3985.356 kb on + strand, within hemY at 3985.379 kb on - strand, within hemY at 3985.383 kb on - strand, within hemY at 3985.383 kb on - strand, within hemY at 3985.394 kb on - strand, within hemY at 3985.425 kb on + strand, within hemY at 3985.425 kb on + strand, within hemY at 3985.430 kb on - strand, within hemY at 3985.488 kb on - strand, within hemY at 3985.498 kb on - strand, within hemY at 3985.536 kb on + strand, within hemY at 3985.536 kb on + strand, within hemY at 3985.544 kb on - strand, within hemY at 3985.544 kb on - strand, within hemY at 3985.544 kb on - strand, within hemY at 3985.546 kb on + strand, within hemY at 3985.554 kb on - strand, within hemY at 3985.643 kb on + strand, within hemY at 3985.778 kb on + strand, within hemY at 3985.799 kb on - strand at 3985.819 kb on - strand at 3985.824 kb on - strand at 3985.905 kb on + strand at 3985.905 kb on + strand at 3985.954 kb on + strand at 3985.957 kb on - strand at 3985.968 kb on - strand at 3985.981 kb on + strand at 3985.981 kb on + strand at 3985.984 kb on + strand at 3985.984 kb on + strand at 3986.026 kb on + strand at 3986.084 kb on + strand, within hemX at 3986.084 kb on + strand, within hemX at 3986.092 kb on - strand, within hemX at 3986.129 kb on + strand, within hemX at 3986.139 kb on + strand, within hemX at 3986.139 kb on + strand, within hemX at 3986.149 kb on - strand, within hemX at 3986.150 kb on + strand, within hemX at 3986.151 kb on + strand, within hemX at 3986.151 kb on + strand, within hemX at 3986.168 kb on + strand, within hemX at 3986.168 kb on + strand, within hemX at 3986.180 kb on + strand, within hemX at 3986.180 kb on + strand, within hemX at 3986.207 kb on + strand, within hemX at 3986.207 kb on + strand, within hemX at 3986.215 kb on - strand, within hemX at 3986.215 kb on - strand, within hemX at 3986.280 kb on - strand, within hemX at 3986.356 kb on + strand, within hemX at 3986.356 kb on + strand, within hemX at 3986.385 kb on - strand, within hemX at 3986.387 kb on + strand, within hemX at 3986.432 kb on - strand, within hemX at 3986.491 kb on + strand, within hemX at 3986.491 kb on + strand, within hemX at 3986.498 kb on - strand, within hemX at 3986.510 kb on + strand, within hemX at 3986.510 kb on + strand, within hemX at 3986.512 kb on + strand, within hemX at 3986.536 kb on + strand, within hemX at 3986.536 kb on + strand, within hemX at 3986.539 kb on - strand, within hemX at 3986.564 kb on - strand, within hemX at 3986.571 kb on + strand, within hemX at 3986.571 kb on + strand, within hemX at 3986.601 kb on + strand, within hemX at 3986.609 kb on + strand, within hemX at 3986.618 kb on - strand, within hemX at 3986.620 kb on + strand, within hemX at 3986.623 kb on + strand, within hemX at 3986.627 kb on + strand, within hemX at 3986.627 kb on + strand, within hemX at 3986.628 kb on + strand, within hemX at 3986.638 kb on + strand, within hemX at 3986.638 kb on + strand, within hemX at 3986.812 kb on - strand, within hemX at 3986.846 kb on - strand, within hemX at 3986.897 kb on + strand, within hemX at 3986.897 kb on + strand, within hemX at 3986.922 kb on + strand, within hemX at 3986.948 kb on + strand, within hemX at 3986.964 kb on - strand, within hemX at 3986.964 kb on - strand, within hemX at 3987.023 kb on + strand at 3987.023 kb on + strand at 3987.027 kb on - strand at 3987.027 kb on - strand at 3987.031 kb on + strand at 3987.033 kb on + strand at 3987.048 kb on + strand at 3987.048 kb on - strand at 3987.051 kb on - strand at 3987.059 kb on - strand at 3987.101 kb on + strand at 3987.114 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 30 mM remove 3,984,908 - hemY b3802 0.17 +1.4 3,984,908 - hemY b3802 0.17 -0.7 3,984,911 + hemY b3802 0.17 +1.4 3,984,911 + hemY b3802 0.17 +0.7 3,984,914 - hemY b3802 0.17 +0.0 3,984,914 - hemY b3802 0.17 -0.6 3,984,926 - hemY b3802 0.18 -0.8 3,984,926 - hemY b3802 0.18 +0.1 3,985,008 + hemY b3802 0.25 -0.8 3,985,041 - hemY b3802 0.28 +0.4 3,985,083 + hemY b3802 0.31 -0.4 3,985,115 + hemY b3802 0.34 +0.2 3,985,115 - hemY b3802 0.34 -0.7 3,985,118 + hemY b3802 0.34 +0.0 3,985,120 + hemY b3802 0.34 +0.2 3,985,121 - hemY b3802 0.34 -0.2 3,985,121 - hemY b3802 0.34 -1.2 3,985,121 - hemY b3802 0.34 +0.4 3,985,146 - hemY b3802 0.37 -0.3 3,985,174 + hemY b3802 0.39 +0.3 3,985,177 - hemY b3802 0.39 +0.5 3,985,189 + hemY b3802 0.40 -1.3 3,985,189 + hemY b3802 0.40 -0.2 3,985,208 + hemY b3802 0.42 +1.4 3,985,208 + hemY b3802 0.42 -1.0 3,985,211 - hemY b3802 0.42 -0.4 3,985,230 - hemY b3802 0.44 +0.1 3,985,230 - hemY b3802 0.44 +1.7 3,985,230 - hemY b3802 0.44 +0.6 3,985,255 - hemY b3802 0.46 +0.2 3,985,255 - hemY b3802 0.46 -1.7 3,985,260 - hemY b3802 0.46 -0.7 3,985,273 + hemY b3802 0.47 +0.1 3,985,287 + hemY b3802 0.48 +0.8 3,985,302 - hemY b3802 0.50 -0.1 3,985,305 - hemY b3802 0.50 +0.6 3,985,305 - hemY b3802 0.50 -0.4 3,985,315 - hemY b3802 0.51 -0.4 3,985,321 - hemY b3802 0.51 -0.2 3,985,321 - hemY b3802 0.51 -1.9 3,985,321 - hemY b3802 0.51 -1.8 3,985,334 - hemY b3802 0.52 -2.2 3,985,334 - hemY b3802 0.52 +1.4 3,985,356 + hemY b3802 0.54 -1.4 3,985,379 - hemY b3802 0.56 -0.6 3,985,383 - hemY b3802 0.56 +1.7 3,985,383 - hemY b3802 0.56 +0.0 3,985,394 - hemY b3802 0.57 -0.4 3,985,425 + hemY b3802 0.60 -1.7 3,985,425 + hemY b3802 0.60 -0.6 3,985,430 - hemY b3802 0.60 -0.1 3,985,488 - hemY b3802 0.65 -1.6 3,985,498 - hemY b3802 0.66 +0.6 3,985,536 + hemY b3802 0.69 +0.9 3,985,536 + hemY b3802 0.69 -0.5 3,985,544 - hemY b3802 0.70 +0.2 3,985,544 - hemY b3802 0.70 -0.4 3,985,544 - hemY b3802 0.70 -0.6 3,985,546 + hemY b3802 0.70 +0.8 3,985,554 - hemY b3802 0.71 +0.2 3,985,643 + hemY b3802 0.78 +0.9 3,985,778 + hemY b3802 0.89 -1.0 3,985,799 - -1.0 3,985,819 - -0.8 3,985,824 - +0.8 3,985,905 + -0.7 3,985,905 + +0.7 3,985,954 + +0.1 3,985,957 - -0.2 3,985,968 - -0.3 3,985,981 + +0.4 3,985,981 + -1.0 3,985,984 + -0.4 3,985,984 + +0.7 3,986,026 + +0.1 3,986,084 + hemX b3803 0.15 -0.3 3,986,084 + hemX b3803 0.15 -0.7 3,986,092 - hemX b3803 0.16 +0.4 3,986,129 + hemX b3803 0.19 -0.6 3,986,139 + hemX b3803 0.20 -0.2 3,986,139 + hemX b3803 0.20 -2.0 3,986,149 - hemX b3803 0.20 -0.6 3,986,150 + hemX b3803 0.20 -0.3 3,986,151 + hemX b3803 0.21 -0.8 3,986,151 + hemX b3803 0.21 -0.8 3,986,168 + hemX b3803 0.22 -0.9 3,986,168 + hemX b3803 0.22 +1.4 3,986,180 + hemX b3803 0.23 +2.0 3,986,180 + hemX b3803 0.23 +1.4 3,986,207 + hemX b3803 0.25 +0.0 3,986,207 + hemX b3803 0.25 -1.9 3,986,215 - hemX b3803 0.26 -0.9 3,986,215 - hemX b3803 0.26 -0.5 3,986,280 - hemX b3803 0.31 -0.3 3,986,356 + hemX b3803 0.38 -0.3 3,986,356 + hemX b3803 0.38 +1.4 3,986,385 - hemX b3803 0.40 -0.7 3,986,387 + hemX b3803 0.41 -0.4 3,986,432 - hemX b3803 0.44 -0.5 3,986,491 + hemX b3803 0.49 -0.9 3,986,491 + hemX b3803 0.49 +0.8 3,986,498 - hemX b3803 0.50 -2.8 3,986,510 + hemX b3803 0.51 +1.4 3,986,510 + hemX b3803 0.51 -1.4 3,986,512 + hemX b3803 0.51 -0.9 3,986,536 + hemX b3803 0.53 -1.7 3,986,536 + hemX b3803 0.53 -0.6 3,986,539 - hemX b3803 0.53 -0.7 3,986,564 - hemX b3803 0.55 -1.8 3,986,571 + hemX b3803 0.56 +0.1 3,986,571 + hemX b3803 0.56 +0.2 3,986,601 + hemX b3803 0.59 +0.2 3,986,609 + hemX b3803 0.59 -0.4 3,986,618 - hemX b3803 0.60 -1.4 3,986,620 + hemX b3803 0.60 -0.6 3,986,623 + hemX b3803 0.60 -1.0 3,986,627 + hemX b3803 0.61 +0.3 3,986,627 + hemX b3803 0.61 +0.1 3,986,628 + hemX b3803 0.61 +0.7 3,986,638 + hemX b3803 0.62 -0.9 3,986,638 + hemX b3803 0.62 +1.4 3,986,812 - hemX b3803 0.76 -0.2 3,986,846 - hemX b3803 0.79 -0.6 3,986,897 + hemX b3803 0.84 -0.4 3,986,897 + hemX b3803 0.84 +0.4 3,986,922 + hemX b3803 0.86 -0.0 3,986,948 + hemX b3803 0.88 -0.7 3,986,964 - hemX b3803 0.89 +0.2 3,986,964 - hemX b3803 0.89 +0.1 3,987,023 + +0.2 3,987,023 + +0.2 3,987,027 - +0.6 3,987,027 - +0.1 3,987,031 + +0.0 3,987,033 + -1.7 3,987,048 + +1.2 3,987,048 - -0.6 3,987,051 - +0.1 3,987,059 - +1.7 3,987,101 + +1.4 3,987,114 + +0.3
Or see this region's nucleotide sequence