Strain Fitness in Escherichia coli BW25113 around b3423

Experiment: 1-ethyl-3-methylimidazolium acetate 30 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	1-ethyl-3-methylimidazolium acetate 30 mM
3,556,933	-	rtcR	b3422	0.40	+0.5
3,556,940	-	rtcR	b3422	0.41	+0.7
3,556,956	+	rtcR	b3422	0.42	+0.1
3,556,973	-	rtcR	b3422	0.43	+0.6
3,556,973	-	rtcR	b3422	0.43	+1.8
3,556,986	-	rtcR	b3422	0.44	+0.5
3,557,003	-	rtcR	b3422	0.45	+2.3
3,557,008	-	rtcR	b3422	0.45	+1.7
3,557,019	+	rtcR	b3422	0.46	+0.3
3,557,029	+	rtcR	b3422	0.46	+0.5
3,557,034	+	rtcR	b3422	0.47	-0.9
3,557,042	-	rtcR	b3422	0.47	+1.0
3,557,042	-	rtcR	b3422	0.47	-0.3
3,557,166	+	rtcR	b3422	0.55	-0.2
3,557,179	-	rtcR	b3422	0.56	-1.3
3,557,179	-	rtcR	b3422	0.56	+0.6
3,557,218	+	rtcR	b3422	0.58	+0.7
3,557,246	+	rtcR	b3422	0.60	+0.3
3,557,246	+	rtcR	b3422	0.60	+0.5
3,557,280	+	rtcR	b3422	0.62	-0.1
3,557,345	+	rtcR	b3422	0.66	+0.6
3,557,345	+	rtcR	b3422	0.66	-0.2
3,557,347	-	rtcR	b3422	0.66	+0.0
3,557,347	-	rtcR	b3422	0.66	+0.4
3,557,368	+	rtcR	b3422	0.67	+1.1
3,557,389	+	rtcR	b3422	0.69	+0.1
3,557,506	-	rtcR	b3422	0.76	-0.5
3,557,560	-	rtcR	b3422	0.79	+0.4
3,557,577	-	rtcR	b3422	0.80	+0.7
3,557,577	-	rtcR	b3422	0.80	+0.7
3,557,583	+	rtcR	b3422	0.81	-0.3
3,557,591	-	rtcR	b3422	0.81	-0.5
3,557,603	-	rtcR	b3422	0.82	+0.6
3,557,621	+	rtcR	b3422	0.83	+1.0
3,557,673	+	rtcR	b3422	0.86	+0.5
3,557,709	-	rtcR	b3422	0.89	-0.2
3,557,709	-	rtcR	b3422	0.89	+0.4
3,557,833	-				+0.6
3,557,849	+				+0.3
3,557,849	+				-1.2
3,557,881	+				+1.3
3,557,884	+				+1.3
3,557,908	-				+0.3
3,557,908	-				+1.3
3,557,948	-	glpR	b3423	0.10	+2.0
3,557,949	+	glpR	b3423	0.10	+0.3
3,557,959	+	glpR	b3423	0.12	-0.1
3,557,968	+	glpR	b3423	0.13	+1.3
3,558,019	+	glpR	b3423	0.20	-0.1
3,558,019	+	glpR	b3423	0.20	-0.2
3,558,069	+	glpR	b3423	0.26	+0.6
3,558,069	+	glpR	b3423	0.26	+0.8
3,558,101	+	glpR	b3423	0.30	+1.1
3,558,103	-	glpR	b3423	0.31	+1.3
3,558,103	-	glpR	b3423	0.31	-0.3
3,558,103	-	glpR	b3423	0.31	-0.2
3,558,104	-	glpR	b3423	0.31	+0.5
3,558,104	-	glpR	b3423	0.31	+0.6
3,558,115	+	glpR	b3423	0.32	+0.9
3,558,143	-	glpR	b3423	0.36	+2.8
3,558,156	-	glpR	b3423	0.38	-0.5
3,558,156	-	glpR	b3423	0.38	+1.7
3,558,221	-	glpR	b3423	0.46	+1.7
3,558,266	+	glpR	b3423	0.52	+0.9
3,558,301	+	glpR	b3423	0.57	+1.3
3,558,309	-	glpR	b3423	0.58	+1.0
3,558,309	-	glpR	b3423	0.58	-0.8
3,558,309	-	glpR	b3423	0.58	+0.8
3,558,309	-	glpR	b3423	0.58	+0.7
3,558,309	-	glpR	b3423	0.58	+2.0
3,558,327	+	glpR	b3423	0.60	+1.1
3,558,364	-	glpR	b3423	0.65	+0.0
3,558,364	-	glpR	b3423	0.65	+0.3
3,558,407	+	glpR	b3423	0.71	-0.3
3,558,409	+	glpR	b3423	0.71	+0.7
3,558,415	+	glpR	b3423	0.72	+0.7
3,558,523	-	glpR	b3423	0.86	+1.5
3,558,572	-				+0.8
3,558,578	+				+0.9
3,558,578	+				+1.3
3,558,588	-				+0.4
3,558,606	+				-0.2
3,558,687	+				+0.4
3,558,753	+	glpG	b3424	0.13	-0.9
3,558,766	+	glpG	b3424	0.15	-0.3
3,558,774	-	glpG	b3424	0.16	+0.6
3,558,776	+	glpG	b3424	0.16	+0.3
3,558,776	+	glpG	b3424	0.16	-0.1
3,558,791	-	glpG	b3424	0.18	+0.2
3,558,794	-	glpG	b3424	0.18	+0.9
3,558,816	-	glpG	b3424	0.21	+0.3
3,558,851	-	glpG	b3424	0.25	+0.3
3,558,855	-	glpG	b3424	0.25	-1.5
3,558,867	-	glpG	b3424	0.27	+0.0
3,558,867	-	glpG	b3424	0.27	+0.3
3,558,919	-	glpG	b3424	0.33	+0.2
3,558,921	-	glpG	b3424	0.33	-1.2
3,558,921	-	glpG	b3424	0.33	-0.6
3,558,927	-	glpG	b3424	0.34	-1.2
3,558,975	+	glpG	b3424	0.40	-0.5
3,558,991	-	glpG	b3424	0.42	+0.2
3,559,003	+	glpG	b3424	0.43	+0.9
3,559,041	-	glpG	b3424	0.48	+2.3
3,559,041	-	glpG	b3424	0.48	+0.5
3,559,049	-	glpG	b3424	0.49	+0.9
3,559,056	+	glpG	b3424	0.49	+0.4
3,559,056	+	glpG	b3424	0.49	+0.3
3,559,058	-	glpG	b3424	0.50	+1.9
3,559,058	-	glpG	b3424	0.50	+1.1
3,559,117	-	glpG	b3424	0.57	-0.6
3,559,127	-	glpG	b3424	0.58	-1.9
3,559,127	-	glpG	b3424	0.58	+0.9
3,559,129	-	glpG	b3424	0.58	+0.5
3,559,160	-	glpG	b3424	0.62	-0.8
3,559,180	+	glpG	b3424	0.64	+0.1
3,559,180	+	glpG	b3424	0.64	+2.0
3,559,185	+	glpG	b3424	0.65	-1.1
3,559,193	+	glpG	b3424	0.66	+0.4
3,559,223	+	glpG	b3424	0.70	-0.7
3,559,223	+	glpG	b3424	0.70	+0.1
3,559,234	-	glpG	b3424	0.71	+0.9
3,559,259	+	glpG	b3424	0.74	+0.3
3,559,262	-	glpG	b3424	0.74	+0.6
3,559,262	-	glpG	b3424	0.74	+1.3
3,559,325	-	glpG	b3424	0.82	+0.1
3,559,342	+	glpG	b3424	0.84	+1.4
3,559,350	-	glpG	b3424	0.85	-0.3
3,559,350	-	glpG	b3424	0.85	+0.4
3,559,350	-	glpG	b3424	0.85	-0.6
3,559,350	-	glpG	b3424	0.85	+0.5
3,559,370	+	glpG	b3424	0.87	+2.3
3,559,370	+	glpG	b3424	0.87	+1.3
3,559,383	-	glpG	b3424	0.89	+0.3
3,559,405	-				+1.3
3,559,405	-				+0.9
3,559,419	-				-1.3
3,559,419	-				+0.3
3,559,439	-				+1.0
3,559,510	+				-1.1
3,559,510	+				+1.5
3,559,518	-				-0.0
3,559,583	+	glpE	b3425	0.19	-1.0
3,559,584	+	glpE	b3425	0.20	+0.5
3,559,587	-	glpE	b3425	0.20	+0.3
3,559,592	-	glpE	b3425	0.22	+0.6
3,559,592	-	glpE	b3425	0.22	+3.3
3,559,603	-	glpE	b3425	0.25	-0.1
3,559,624	-	glpE	b3425	0.32	+1.3