Strain Fitness in Escherichia coli BW25113 around b2873

Experiment: 1-ethyl-3-methylimidazolium acetate 30 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntygeY and b2873 are separated by 52 nucleotidesb2873 and yqeA are separated by 47 nucleotides b2872: ygeY - hypothetical protein (NCBI), at 3,006,786 to 3,007,997 ygeY b2873: b2873 - orf, hypothetical protein (VIMSS), at 3,008,050 to 3,009,435 b2873 b2874: yqeA - predicted amino acid kinase (NCBI), at 3,009,483 to 3,010,415 yqeA Position (kb) 3008 3009 3010Strain fitness (log2 ratio) -2 -1 0 1 2at 3007.137 kb on + strand, within ygeYat 3007.211 kb on + strand, within ygeYat 3007.211 kb on + strand, within ygeYat 3007.316 kb on + strand, within ygeYat 3007.693 kb on - strand, within ygeYat 3007.693 kb on - strand, within ygeYat 3007.695 kb on + strand, within ygeYat 3007.703 kb on - strand, within ygeYat 3007.777 kb on - strand, within ygeYat 3007.884 kb on - strandat 3008.085 kb on - strandat 3008.085 kb on - strandat 3008.382 kb on + strand, within b2873at 3008.434 kb on - strand, within b2873at 3008.434 kb on - strand, within b2873at 3008.751 kb on + strand, within b2873at 3008.751 kb on + strand, within b2873at 3008.751 kb on + strand, within b2873at 3008.751 kb on + strand, within b2873at 3008.759 kb on + strand, within b2873at 3008.782 kb on + strand, within b2873at 3008.782 kb on + strand, within b2873at 3008.785 kb on - strand, within b2873at 3008.790 kb on - strand, within b2873at 3008.836 kb on - strand, within b2873at 3008.836 kb on - strand, within b2873at 3008.878 kb on + strand, within b2873at 3008.984 kb on - strand, within b2873at 3008.984 kb on - strand, within b2873at 3009.015 kb on - strand, within b2873at 3009.015 kb on - strand, within b2873at 3009.238 kb on + strand, within b2873at 3009.238 kb on + strand, within b2873at 3009.253 kb on - strand, within b2873at 3009.276 kb on - strand, within b2873at 3009.288 kb on - strand, within b2873at 3009.323 kb on - strandat 3009.331 kb on + strandat 3009.437 kb on - strandat 3009.437 kb on - strandat 3009.437 kb on - strandat 3009.449 kb on + strandat 3009.449 kb on + strandat 3009.541 kb on + strandat 3009.654 kb on + strand, within yqeAat 3009.688 kb on + strand, within yqeAat 3009.701 kb on - strand, within yqeAat 3009.701 kb on - strand, within yqeAat 3009.730 kb on + strand, within yqeAat 3009.816 kb on - strand, within yqeAat 3009.822 kb on - strand, within yqeAat 3009.922 kb on - strand, within yqeAat 3010.002 kb on + strand, within yqeAat 3010.013 kb on + strand, within yqeAat 3010.232 kb on + strand, within yqeAat 3010.232 kb on + strand, within yqeAat 3010.280 kb on + strand, within yqeAat 3010.282 kb on + strand, within yqeAat 3010.282 kb on + strand, within yqeAat 3010.349 kb on + strandat 3010.390 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 30 mM
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3,007,137 + ygeY b2872 0.29 +0.0
3,007,211 + ygeY b2872 0.35 -0.2
3,007,211 + ygeY b2872 0.35 -0.4
3,007,316 + ygeY b2872 0.44 +0.7
3,007,693 - ygeY b2872 0.75 +0.7
3,007,693 - ygeY b2872 0.75 -0.2
3,007,695 + ygeY b2872 0.75 +2.0
3,007,703 - ygeY b2872 0.76 -0.2
3,007,777 - ygeY b2872 0.82 +0.2
3,007,884 - +0.7
3,008,085 - +0.7
3,008,085 - -0.4
3,008,382 + b2873 b2873 0.24 +0.2
3,008,434 - b2873 b2873 0.28 +0.0
3,008,434 - b2873 b2873 0.28 +0.7
3,008,751 + b2873 b2873 0.51 +1.3
3,008,751 + b2873 b2873 0.51 +0.7
3,008,751 + b2873 b2873 0.51 +0.7
3,008,751 + b2873 b2873 0.51 -0.8
3,008,759 + b2873 b2873 0.51 -0.9
3,008,782 + b2873 b2873 0.53 -0.8
3,008,782 + b2873 b2873 0.53 +0.4
3,008,785 - b2873 b2873 0.53 +0.4
3,008,790 - b2873 b2873 0.53 -0.0
3,008,836 - b2873 b2873 0.57 +0.3
3,008,836 - b2873 b2873 0.57 -0.2
3,008,878 + b2873 b2873 0.60 +0.8
3,008,984 - b2873 b2873 0.67 +1.3
3,008,984 - b2873 b2873 0.67 -0.8
3,009,015 - b2873 b2873 0.70 +1.6
3,009,015 - b2873 b2873 0.70 -0.3
3,009,238 + b2873 b2873 0.86 -0.4
3,009,238 + b2873 b2873 0.86 -0.6
3,009,253 - b2873 b2873 0.87 +0.7
3,009,276 - b2873 b2873 0.88 -2.3
3,009,288 - b2873 b2873 0.89 +1.0
3,009,323 - -0.3
3,009,331 + +0.6
3,009,437 - +0.4
3,009,437 - +0.2
3,009,437 - +2.0
3,009,449 + -0.3
3,009,449 + -0.2
3,009,541 + +0.5
3,009,654 + yqeA b2874 0.18 -0.1
3,009,688 + yqeA b2874 0.22 +0.3
3,009,701 - yqeA b2874 0.23 -0.1
3,009,701 - yqeA b2874 0.23 -0.2
3,009,730 + yqeA b2874 0.26 +0.8
3,009,816 - yqeA b2874 0.36 -0.0
3,009,822 - yqeA b2874 0.36 +0.4
3,009,922 - yqeA b2874 0.47 -0.4
3,010,002 + yqeA b2874 0.56 +0.2
3,010,013 + yqeA b2874 0.57 -0.6
3,010,232 + yqeA b2874 0.80 +1.0
3,010,232 + yqeA b2874 0.80 +1.5
3,010,280 + yqeA b2874 0.85 +1.0
3,010,282 + yqeA b2874 0.86 -0.2
3,010,282 + yqeA b2874 0.86 +0.3
3,010,349 + +0.3
3,010,390 + +0.2

Or see this region's nucleotide sequence