Experiment: 1-ethyl-3-methylimidazolium acetate 30 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt b2430 and b2431 are separated by 239 nucleotides b2431 and yfeY are separated by 95 nucleotides yfeY and b2433 are separated by 60 nucleotides
b2430: b2430 - putative beta-lactamase (VIMSS), at 2,546,124 to 2,547,428
b2430
b2431: b2431 - orf, hypothetical protein (VIMSS), at 2,547,668 to 2,548,567
b2431
b2432: yfeY - hypothetical protein (NCBI), at 2,548,663 to 2,549,238
yfeY
b2433: b2433 - orf, hypothetical protein (VIMSS), at 2,549,299 to 2,549,748
b2433
Position (kb)
2547
2548
2549 Strain fitness (log2 ratio)
-2
-1
0
1 at 2546.674 kb on + strand, within b2430 at 2546.758 kb on + strand, within b2430 at 2546.844 kb on + strand, within b2430 at 2546.969 kb on - strand, within b2430 at 2546.969 kb on - strand, within b2430 at 2547.030 kb on + strand, within b2430 at 2547.062 kb on - strand, within b2430 at 2547.062 kb on - strand, within b2430 at 2547.071 kb on - strand, within b2430 at 2547.087 kb on - strand, within b2430 at 2547.141 kb on - strand, within b2430 at 2547.319 kb on - strand at 2547.338 kb on - strand at 2547.495 kb on + strand at 2547.495 kb on + strand at 2547.774 kb on + strand, within b2431 at 2547.790 kb on + strand, within b2431 at 2547.798 kb on - strand, within b2431 at 2547.813 kb on + strand, within b2431 at 2547.824 kb on + strand, within b2431 at 2547.827 kb on - strand, within b2431 at 2547.830 kb on - strand, within b2431 at 2547.834 kb on + strand, within b2431 at 2547.929 kb on - strand at 2547.929 kb on - strand, within b2431 at 2547.937 kb on - strand, within b2431 at 2547.937 kb on - strand, within b2431 at 2547.991 kb on + strand, within b2431 at 2547.999 kb on - strand, within b2431 at 2548.032 kb on - strand, within b2431 at 2548.032 kb on - strand, within b2431 at 2548.044 kb on - strand, within b2431 at 2548.054 kb on + strand, within b2431 at 2548.054 kb on + strand, within b2431 at 2548.072 kb on + strand, within b2431 at 2548.163 kb on + strand, within b2431 at 2548.201 kb on + strand, within b2431 at 2548.203 kb on + strand, within b2431 at 2548.229 kb on - strand, within b2431 at 2548.322 kb on - strand, within b2431 at 2548.537 kb on - strand at 2548.537 kb on - strand at 2548.895 kb on + strand, within yfeY at 2549.078 kb on + strand, within yfeY at 2549.078 kb on + strand, within yfeY at 2549.210 kb on + strand at 2549.253 kb on - strand at 2549.266 kb on - strand at 2549.348 kb on + strand, within b2433 at 2549.388 kb on + strand, within b2433
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 30 mM remove 2,546,674 + b2430 b2430 0.42 +0.2 2,546,758 + b2430 b2430 0.49 +0.6 2,546,844 + b2430 b2430 0.55 +0.2 2,546,969 - b2430 b2430 0.65 -0.7 2,546,969 - b2430 b2430 0.65 +0.9 2,547,030 + b2430 b2430 0.69 +0.7 2,547,062 - b2430 b2430 0.72 +1.6 2,547,062 - b2430 b2430 0.72 -0.1 2,547,071 - b2430 b2430 0.73 -0.1 2,547,087 - b2430 b2430 0.74 +0.7 2,547,141 - b2430 b2430 0.78 +0.6 2,547,319 - -0.1 2,547,338 - +0.5 2,547,495 + +1.2 2,547,495 + -0.5 2,547,774 + b2431 b2431 0.12 -0.5 2,547,790 + b2431 b2431 0.14 -0.5 2,547,798 - b2431 b2431 0.14 -0.9 2,547,813 + b2431 b2431 0.16 -1.1 2,547,824 + b2431 b2431 0.17 +0.6 2,547,827 - b2431 b2431 0.18 +0.1 2,547,830 - b2431 b2431 0.18 -2.5 2,547,834 + b2431 b2431 0.18 +1.2 2,547,929 - +0.9 2,547,929 - b2431 b2431 0.29 +0.3 2,547,937 - b2431 b2431 0.30 -0.7 2,547,937 - b2431 b2431 0.30 -0.8 2,547,991 + b2431 b2431 0.36 -0.9 2,547,999 - b2431 b2431 0.37 +0.4 2,548,032 - b2431 b2431 0.40 -0.3 2,548,032 - b2431 b2431 0.40 -1.4 2,548,044 - b2431 b2431 0.42 -2.1 2,548,054 + b2431 b2431 0.43 -0.1 2,548,054 + b2431 b2431 0.43 -0.7 2,548,072 + b2431 b2431 0.45 -0.8 2,548,163 + b2431 b2431 0.55 -1.1 2,548,201 + b2431 b2431 0.59 -0.7 2,548,203 + b2431 b2431 0.59 -1.5 2,548,229 - b2431 b2431 0.62 -1.5 2,548,322 - b2431 b2431 0.73 -0.1 2,548,537 - -1.9 2,548,537 - -0.7 2,548,895 + yfeY b2432 0.40 +1.0 2,549,078 + yfeY b2432 0.72 -0.7 2,549,078 + yfeY b2432 0.72 +0.4 2,549,210 + -0.7 2,549,253 - +1.2 2,549,266 - -0.7 2,549,348 + b2433 b2433 0.11 +0.6 2,549,388 + b2433 b2433 0.20 +0.3
Or see this region's nucleotide sequence