Experiment: 1-ethyl-3-methylimidazolium acetate 30 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yejH and rplY are separated by 124 nucleotides rplY and yejK are separated by 138 nucleotides yejK and yejL are separated by 181 nucleotides yejL and yejM are separated by 19 nucleotides
b2184: yejH - DNA damage response helicase (yejH or radD) (from data) , at 2,278,654 to 2,280,414
yejH
b2185: rplY - 50S ribosomal protein L25 (NCBI), at 2,280,539 to 2,280,823
rplY
b2186: yejK - nucleoid-associated protein NdpA (NCBI), at 2,280,962 to 2,281,969
yejK
b2187: yejL - hypothetical protein (NCBI), at 2,282,151 to 2,282,378
yejL
b2188: yejM - predicted hydrolase, inner membrane (NCBI), at 2,282,398 to 2,284,158
yejM
Position (kb)
2280
2281
2282 Strain fitness (log2 ratio)
-2
-1
0
1 at 2279.967 kb on - strand, within yejH at 2280.065 kb on + strand, within yejH at 2280.078 kb on - strand, within yejH at 2280.078 kb on - strand, within yejH at 2280.080 kb on + strand, within yejH at 2280.148 kb on + strand, within yejH at 2280.161 kb on + strand, within yejH at 2280.214 kb on - strand, within yejH at 2280.240 kb on + strand at 2280.266 kb on - strand at 2280.943 kb on + strand at 2281.099 kb on - strand, within yejK at 2281.099 kb on - strand, within yejK at 2281.135 kb on + strand, within yejK at 2281.166 kb on - strand, within yejK at 2281.166 kb on - strand, within yejK at 2281.253 kb on + strand, within yejK at 2281.255 kb on + strand, within yejK at 2281.263 kb on + strand, within yejK at 2281.263 kb on + strand, within yejK at 2281.284 kb on - strand, within yejK at 2281.284 kb on - strand, within yejK at 2281.286 kb on + strand, within yejK at 2281.294 kb on - strand, within yejK at 2281.323 kb on - strand, within yejK at 2281.325 kb on + strand, within yejK at 2281.347 kb on + strand, within yejK at 2281.377 kb on - strand, within yejK at 2281.447 kb on + strand, within yejK at 2281.456 kb on - strand, within yejK at 2281.483 kb on + strand, within yejK at 2281.512 kb on - strand, within yejK at 2281.512 kb on - strand, within yejK at 2281.780 kb on - strand, within yejK at 2281.887 kb on + strand at 2281.905 kb on - strand at 2282.004 kb on - strand at 2282.026 kb on + strand at 2282.026 kb on + strand at 2282.256 kb on + strand, within yejL at 2282.304 kb on + strand, within yejL
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 30 mM remove 2,279,967 - yejH b2184 0.75 +0.4 2,280,065 + yejH b2184 0.80 +0.9 2,280,078 - yejH b2184 0.81 +0.5 2,280,078 - yejH b2184 0.81 -0.4 2,280,080 + yejH b2184 0.81 +0.9 2,280,148 + yejH b2184 0.85 -0.7 2,280,161 + yejH b2184 0.86 +0.6 2,280,214 - yejH b2184 0.89 +0.6 2,280,240 + +0.1 2,280,266 - -0.0 2,280,943 + +0.4 2,281,099 - yejK b2186 0.14 +1.4 2,281,099 - yejK b2186 0.14 +0.9 2,281,135 + yejK b2186 0.17 +0.2 2,281,166 - yejK b2186 0.20 -2.4 2,281,166 - yejK b2186 0.20 +0.2 2,281,253 + yejK b2186 0.29 -0.7 2,281,255 + yejK b2186 0.29 +0.8 2,281,263 + yejK b2186 0.30 +0.5 2,281,263 + yejK b2186 0.30 +0.7 2,281,284 - yejK b2186 0.32 +1.2 2,281,284 - yejK b2186 0.32 +0.9 2,281,286 + yejK b2186 0.32 +0.7 2,281,294 - yejK b2186 0.33 +0.1 2,281,323 - yejK b2186 0.36 +0.6 2,281,325 + yejK b2186 0.36 +0.2 2,281,347 + yejK b2186 0.38 +1.1 2,281,377 - yejK b2186 0.41 +0.7 2,281,447 + yejK b2186 0.48 +0.9 2,281,456 - yejK b2186 0.49 +0.6 2,281,483 + yejK b2186 0.52 +1.1 2,281,512 - yejK b2186 0.55 +1.0 2,281,512 - yejK b2186 0.55 +0.5 2,281,780 - yejK b2186 0.81 +0.5 2,281,887 + +0.9 2,281,905 - +0.9 2,282,004 - +0.6 2,282,026 + -0.9 2,282,026 + +0.1 2,282,256 + yejL b2187 0.46 +0.1 2,282,304 + yejL b2187 0.67 +0.4
Or see this region's nucleotide sequence