Experiment: 1-ethyl-3-methylimidazolium acetate 30 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yehZ and bglX are separated by 210 nucleotides bglX and dld are separated by 195 nucleotides
b2131: yehZ - predicted transporter subunit: periplasmic-binding component of ABC superfamily (NCBI), at 2,216,586 to 2,217,503
yehZ
b2132: bglX - beta-D-glucoside glucohydrolase, periplasmic (NCBI), at 2,217,714 to 2,220,011
bglX
b2133: dld - D-lactate dehydrogenase, FAD-binding, NADH independent (NCBI), at 2,220,207 to 2,221,922
dld
Position (kb)
2217
2218
2219
2220
2221 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2216.745 kb on - strand, within yehZ at 2216.745 kb on - strand, within yehZ at 2216.748 kb on - strand, within yehZ at 2216.767 kb on - strand, within yehZ at 2216.782 kb on + strand, within yehZ at 2216.950 kb on - strand, within yehZ at 2216.950 kb on - strand, within yehZ at 2216.955 kb on + strand, within yehZ at 2216.955 kb on + strand, within yehZ at 2217.019 kb on + strand, within yehZ at 2217.153 kb on - strand, within yehZ at 2217.153 kb on - strand, within yehZ at 2217.260 kb on + strand, within yehZ at 2217.311 kb on - strand, within yehZ at 2217.311 kb on - strand, within yehZ at 2217.328 kb on + strand, within yehZ at 2217.376 kb on + strand, within yehZ at 2217.463 kb on + strand at 2217.470 kb on + strand at 2217.560 kb on - strand at 2217.621 kb on - strand at 2217.682 kb on - strand at 2217.769 kb on + strand at 2218.066 kb on + strand, within bglX at 2218.089 kb on + strand, within bglX at 2218.089 kb on + strand, within bglX at 2218.305 kb on - strand, within bglX at 2218.470 kb on + strand, within bglX at 2218.470 kb on + strand, within bglX at 2218.476 kb on - strand, within bglX at 2218.476 kb on - strand, within bglX at 2218.483 kb on - strand, within bglX at 2218.483 kb on - strand, within bglX at 2218.544 kb on + strand, within bglX at 2218.544 kb on + strand, within bglX at 2218.688 kb on - strand, within bglX at 2218.688 kb on - strand, within bglX at 2218.767 kb on - strand, within bglX at 2218.871 kb on - strand, within bglX at 2218.871 kb on - strand, within bglX at 2218.974 kb on - strand, within bglX at 2219.011 kb on + strand, within bglX at 2219.011 kb on + strand, within bglX at 2219.038 kb on + strand, within bglX at 2219.038 kb on + strand, within bglX at 2219.043 kb on + strand, within bglX at 2219.130 kb on - strand, within bglX at 2219.130 kb on - strand, within bglX at 2219.140 kb on - strand, within bglX at 2219.307 kb on - strand, within bglX at 2219.343 kb on + strand, within bglX at 2219.398 kb on + strand, within bglX at 2219.398 kb on + strand, within bglX at 2219.407 kb on + strand, within bglX at 2219.415 kb on - strand, within bglX at 2219.539 kb on - strand, within bglX at 2219.559 kb on - strand, within bglX at 2219.659 kb on - strand, within bglX at 2219.664 kb on - strand, within bglX at 2219.891 kb on + strand at 2219.891 kb on + strand at 2219.969 kb on + strand at 2219.969 kb on + strand at 2219.977 kb on - strand at 2219.978 kb on - strand at 2219.998 kb on - strand at 2220.036 kb on - strand at 2220.147 kb on + strand at 2220.147 kb on + strand at 2220.167 kb on - strand at 2220.167 kb on - strand at 2220.206 kb on - strand at 2220.206 kb on - strand at 2220.220 kb on - strand at 2220.276 kb on - strand at 2220.276 kb on - strand at 2220.279 kb on - strand at 2220.330 kb on + strand at 2220.560 kb on - strand, within dld at 2220.623 kb on + strand, within dld at 2220.635 kb on + strand, within dld at 2220.651 kb on - strand, within dld at 2220.781 kb on + strand, within dld at 2220.781 kb on + strand, within dld at 2220.784 kb on - strand, within dld at 2220.784 kb on - strand, within dld at 2220.811 kb on + strand, within dld
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 30 mM remove 2,216,745 - yehZ b2131 0.17 -0.3 2,216,745 - yehZ b2131 0.17 -0.5 2,216,748 - yehZ b2131 0.18 +0.1 2,216,767 - yehZ b2131 0.20 +0.1 2,216,782 + yehZ b2131 0.21 -0.3 2,216,950 - yehZ b2131 0.40 +0.0 2,216,950 - yehZ b2131 0.40 -0.4 2,216,955 + yehZ b2131 0.40 -0.1 2,216,955 + yehZ b2131 0.40 +0.9 2,217,019 + yehZ b2131 0.47 -0.0 2,217,153 - yehZ b2131 0.62 +1.6 2,217,153 - yehZ b2131 0.62 -2.1 2,217,260 + yehZ b2131 0.73 +1.3 2,217,311 - yehZ b2131 0.79 +1.2 2,217,311 - yehZ b2131 0.79 +1.9 2,217,328 + yehZ b2131 0.81 +0.0 2,217,376 + yehZ b2131 0.86 +1.6 2,217,463 + +0.7 2,217,470 + -0.5 2,217,560 - +0.5 2,217,621 - +0.0 2,217,682 - +0.1 2,217,769 + -1.0 2,218,066 + bglX b2132 0.15 +1.3 2,218,089 + bglX b2132 0.16 -0.4 2,218,089 + bglX b2132 0.16 -0.2 2,218,305 - bglX b2132 0.26 +0.3 2,218,470 + bglX b2132 0.33 -1.6 2,218,470 + bglX b2132 0.33 -1.5 2,218,476 - bglX b2132 0.33 +0.2 2,218,476 - bglX b2132 0.33 -0.1 2,218,483 - bglX b2132 0.33 -1.3 2,218,483 - bglX b2132 0.33 +0.0 2,218,544 + bglX b2132 0.36 -0.5 2,218,544 + bglX b2132 0.36 -1.7 2,218,688 - bglX b2132 0.42 -1.7 2,218,688 - bglX b2132 0.42 -1.1 2,218,767 - bglX b2132 0.46 -2.1 2,218,871 - bglX b2132 0.50 +0.4 2,218,871 - bglX b2132 0.50 -1.7 2,218,974 - bglX b2132 0.55 -0.9 2,219,011 + bglX b2132 0.56 -0.9 2,219,011 + bglX b2132 0.56 -0.7 2,219,038 + bglX b2132 0.58 +0.1 2,219,038 + bglX b2132 0.58 -0.5 2,219,043 + bglX b2132 0.58 -2.3 2,219,130 - bglX b2132 0.62 +0.1 2,219,130 - bglX b2132 0.62 -0.4 2,219,140 - bglX b2132 0.62 -0.1 2,219,307 - bglX b2132 0.69 -0.6 2,219,343 + bglX b2132 0.71 +0.9 2,219,398 + bglX b2132 0.73 +0.7 2,219,398 + bglX b2132 0.73 -0.1 2,219,407 + bglX b2132 0.74 +0.2 2,219,415 - bglX b2132 0.74 +0.9 2,219,539 - bglX b2132 0.79 -2.2 2,219,559 - bglX b2132 0.80 -0.9 2,219,659 - bglX b2132 0.85 +0.1 2,219,664 - bglX b2132 0.85 -0.7 2,219,891 + -0.6 2,219,891 + +0.3 2,219,969 + -0.1 2,219,969 + +0.1 2,219,977 - +0.7 2,219,978 - -0.7 2,219,998 - +0.0 2,220,036 - -0.0 2,220,147 + +1.6 2,220,147 + +0.9 2,220,167 - +2.1 2,220,167 - -1.1 2,220,206 - -0.1 2,220,206 - +0.1 2,220,220 - +0.6 2,220,276 - +1.2 2,220,276 - +0.1 2,220,279 - -0.6 2,220,330 + +0.1 2,220,560 - dld b2133 0.21 -0.1 2,220,623 + dld b2133 0.24 +1.4 2,220,635 + dld b2133 0.25 -0.4 2,220,651 - dld b2133 0.26 +0.2 2,220,781 + dld b2133 0.33 +0.1 2,220,781 + dld b2133 0.33 +0.3 2,220,784 - dld b2133 0.34 +0.9 2,220,784 - dld b2133 0.34 +1.9 2,220,811 + dld b2133 0.35 -0.7
Or see this region's nucleotide sequence