Experiment: 1-ethyl-3-methylimidazolium acetate 30 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaM and wcaL are separated by 10 nucleotides wcaL and wcaK overlap by 4 nucleotides
b2043: wcaM - predicted colanic acid biosynthesis protein (NCBI), at 2,112,526 to 2,113,920
wcaM
b2044: wcaL - predicted glycosyl transferase (NCBI), at 2,113,931 to 2,115,151
wcaL
b2045: wcaK - predicted pyruvyl transferase (NCBI), at 2,115,148 to 2,116,428
wcaK
Position (kb)
2113
2114
2115
2116 Strain fitness (log2 ratio)
-1
0
1
2 at 2112.965 kb on - strand, within wcaM at 2112.965 kb on - strand, within wcaM at 2112.973 kb on - strand, within wcaM at 2113.022 kb on + strand, within wcaM at 2113.024 kb on + strand, within wcaM at 2113.050 kb on - strand, within wcaM at 2113.050 kb on - strand, within wcaM at 2113.060 kb on - strand, within wcaM at 2113.074 kb on - strand, within wcaM at 2113.125 kb on + strand, within wcaM at 2113.125 kb on + strand, within wcaM at 2113.188 kb on - strand, within wcaM at 2113.197 kb on - strand, within wcaM at 2113.207 kb on - strand, within wcaM at 2113.225 kb on - strand, within wcaM at 2113.233 kb on + strand, within wcaM at 2113.234 kb on + strand, within wcaM at 2113.234 kb on + strand, within wcaM at 2113.241 kb on - strand, within wcaM at 2113.242 kb on - strand, within wcaM at 2113.242 kb on - strand, within wcaM at 2113.263 kb on + strand, within wcaM at 2113.271 kb on + strand, within wcaM at 2113.274 kb on - strand, within wcaM at 2113.290 kb on + strand, within wcaM at 2113.290 kb on + strand, within wcaM at 2113.290 kb on + strand, within wcaM at 2113.338 kb on - strand, within wcaM at 2113.346 kb on - strand, within wcaM at 2113.357 kb on - strand, within wcaM at 2113.388 kb on - strand, within wcaM at 2113.414 kb on + strand, within wcaM at 2113.421 kb on - strand, within wcaM at 2113.421 kb on - strand, within wcaM at 2113.422 kb on - strand, within wcaM at 2113.435 kb on - strand, within wcaM at 2113.523 kb on - strand, within wcaM at 2113.530 kb on + strand, within wcaM at 2113.691 kb on + strand, within wcaM at 2113.691 kb on - strand, within wcaM at 2113.750 kb on - strand, within wcaM at 2113.750 kb on - strand, within wcaM at 2113.926 kb on + strand at 2114.118 kb on + strand, within wcaL at 2114.118 kb on + strand, within wcaL at 2114.251 kb on - strand, within wcaL at 2114.539 kb on - strand, within wcaL at 2114.789 kb on - strand, within wcaL at 2114.874 kb on - strand, within wcaL at 2115.177 kb on - strand at 2115.320 kb on + strand, within wcaK at 2115.414 kb on - strand, within wcaK at 2115.426 kb on - strand, within wcaK at 2115.426 kb on - strand, within wcaK at 2115.441 kb on + strand, within wcaK at 2115.507 kb on - strand, within wcaK at 2115.528 kb on + strand, within wcaK at 2115.548 kb on + strand, within wcaK at 2115.584 kb on + strand, within wcaK at 2115.585 kb on - strand, within wcaK at 2115.603 kb on - strand, within wcaK at 2115.651 kb on + strand, within wcaK at 2115.660 kb on + strand, within wcaK at 2115.708 kb on + strand, within wcaK at 2115.716 kb on - strand, within wcaK at 2115.754 kb on - strand, within wcaK at 2115.790 kb on + strand, within wcaK at 2115.941 kb on + strand, within wcaK at 2115.941 kb on + strand, within wcaK at 2115.949 kb on - strand, within wcaK at 2115.959 kb on - strand, within wcaK at 2115.959 kb on - strand, within wcaK at 2115.968 kb on + strand, within wcaK at 2115.968 kb on + strand, within wcaK at 2115.976 kb on - strand, within wcaK at 2115.995 kb on + strand, within wcaK at 2116.108 kb on + strand, within wcaK at 2116.108 kb on + strand, within wcaK
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 30 mM remove 2,112,965 - wcaM b2043 0.31 +1.2 2,112,965 - wcaM b2043 0.31 +0.7 2,112,973 - wcaM b2043 0.32 +0.2 2,113,022 + wcaM b2043 0.36 +1.1 2,113,024 + wcaM b2043 0.36 +0.7 2,113,050 - wcaM b2043 0.38 +0.3 2,113,050 - wcaM b2043 0.38 +0.3 2,113,060 - wcaM b2043 0.38 -0.4 2,113,074 - wcaM b2043 0.39 +1.1 2,113,125 + wcaM b2043 0.43 -0.2 2,113,125 + wcaM b2043 0.43 -1.7 2,113,188 - wcaM b2043 0.47 +0.1 2,113,197 - wcaM b2043 0.48 +1.4 2,113,207 - wcaM b2043 0.49 -1.2 2,113,225 - wcaM b2043 0.50 -0.7 2,113,233 + wcaM b2043 0.51 -0.2 2,113,234 + wcaM b2043 0.51 +1.3 2,113,234 + wcaM b2043 0.51 -0.2 2,113,241 - wcaM b2043 0.51 -0.6 2,113,242 - wcaM b2043 0.51 -0.6 2,113,242 - wcaM b2043 0.51 +0.3 2,113,263 + wcaM b2043 0.53 -1.0 2,113,271 + wcaM b2043 0.53 -1.0 2,113,274 - wcaM b2043 0.54 +1.1 2,113,290 + wcaM b2043 0.55 -0.6 2,113,290 + wcaM b2043 0.55 +1.7 2,113,290 + wcaM b2043 0.55 -0.2 2,113,338 - wcaM b2043 0.58 +1.6 2,113,346 - wcaM b2043 0.59 -0.1 2,113,357 - wcaM b2043 0.60 -0.4 2,113,388 - wcaM b2043 0.62 +1.3 2,113,414 + wcaM b2043 0.64 -0.5 2,113,421 - wcaM b2043 0.64 -0.7 2,113,421 - wcaM b2043 0.64 -0.6 2,113,422 - wcaM b2043 0.64 -0.2 2,113,435 - wcaM b2043 0.65 +0.3 2,113,523 - wcaM b2043 0.71 +0.3 2,113,530 + wcaM b2043 0.72 -0.3 2,113,691 + wcaM b2043 0.84 -0.8 2,113,691 - wcaM b2043 0.84 +1.8 2,113,750 - wcaM b2043 0.88 -0.3 2,113,750 - wcaM b2043 0.88 +1.4 2,113,926 + -0.1 2,114,118 + wcaL b2044 0.15 +1.6 2,114,118 + wcaL b2044 0.15 +1.1 2,114,251 - wcaL b2044 0.26 +0.6 2,114,539 - wcaL b2044 0.50 +0.4 2,114,789 - wcaL b2044 0.70 +0.1 2,114,874 - wcaL b2044 0.77 +1.8 2,115,177 - +0.3 2,115,320 + wcaK b2045 0.13 -0.9 2,115,414 - wcaK b2045 0.21 +0.3 2,115,426 - wcaK b2045 0.22 +0.5 2,115,426 - wcaK b2045 0.22 +1.8 2,115,441 + wcaK b2045 0.23 +0.8 2,115,507 - wcaK b2045 0.28 -0.7 2,115,528 + wcaK b2045 0.30 +0.2 2,115,548 + wcaK b2045 0.31 +0.8 2,115,584 + wcaK b2045 0.34 +1.3 2,115,585 - wcaK b2045 0.34 +0.5 2,115,603 - wcaK b2045 0.36 -0.1 2,115,651 + wcaK b2045 0.39 +0.5 2,115,660 + wcaK b2045 0.40 -0.3 2,115,708 + wcaK b2045 0.44 -0.5 2,115,716 - wcaK b2045 0.44 +0.6 2,115,754 - wcaK b2045 0.47 +0.6 2,115,790 + wcaK b2045 0.50 +0.3 2,115,941 + wcaK b2045 0.62 +0.6 2,115,941 + wcaK b2045 0.62 -0.5 2,115,949 - wcaK b2045 0.63 -1.0 2,115,959 - wcaK b2045 0.63 -1.0 2,115,959 - wcaK b2045 0.63 -1.6 2,115,968 + wcaK b2045 0.64 -0.8 2,115,968 + wcaK b2045 0.64 +0.7 2,115,976 - wcaK b2045 0.65 +2.6 2,115,995 + wcaK b2045 0.66 -0.4 2,116,108 + wcaK b2045 0.75 +0.3 2,116,108 + wcaK b2045 0.75 -0.5
Or see this region's nucleotide sequence