Experiment: 1-ethyl-3-methylimidazolium acetate 30 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yciA and yciB are separated by 104 nucleotides yciB and yciC are separated by 29 nucleotides yciC and ompW are separated by 356 nucleotides
b1253: yciA - predicted hydrolase (NCBI), at 1,309,872 to 1,310,270
yciA
b1254: yciB - intracellular septation protein A (NCBI), at 1,310,375 to 1,310,914
yciB
b1255: yciC - hypothetical protein (NCBI), at 1,310,944 to 1,311,687
yciC
b1256: ompW - outer membrane protein W (NCBI), at 1,312,044 to 1,312,682
ompW
Position (kb)
1310
1311
1312 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1310.036 kb on + strand, within yciA at 1310.036 kb on + strand, within yciA at 1310.046 kb on + strand, within yciA at 1310.061 kb on + strand, within yciA at 1310.141 kb on - strand, within yciA at 1310.410 kb on + strand at 1310.416 kb on - strand at 1310.650 kb on - strand, within yciB at 1310.809 kb on - strand, within yciB at 1311.105 kb on + strand, within yciC at 1311.105 kb on + strand, within yciC at 1311.187 kb on - strand, within yciC at 1311.189 kb on + strand, within yciC at 1311.212 kb on - strand, within yciC at 1311.212 kb on - strand, within yciC at 1311.329 kb on - strand, within yciC at 1311.380 kb on + strand, within yciC at 1311.448 kb on - strand, within yciC at 1311.448 kb on - strand, within yciC at 1311.621 kb on - strand at 1311.661 kb on + strand at 1311.670 kb on - strand at 1311.670 kb on - strand at 1311.670 kb on - strand at 1311.671 kb on - strand at 1311.677 kb on + strand at 1311.691 kb on + strand at 1311.691 kb on - strand at 1311.714 kb on + strand at 1311.747 kb on - strand at 1311.747 kb on - strand at 1311.747 kb on - strand at 1311.772 kb on - strand at 1311.858 kb on - strand at 1311.973 kb on + strand at 1311.973 kb on + strand at 1312.034 kb on - strand at 1312.096 kb on - strand at 1312.146 kb on + strand, within ompW at 1312.234 kb on + strand, within ompW at 1312.234 kb on + strand, within ompW at 1312.237 kb on - strand, within ompW at 1312.248 kb on + strand, within ompW at 1312.369 kb on + strand, within ompW at 1312.369 kb on + strand, within ompW at 1312.372 kb on - strand, within ompW at 1312.387 kb on + strand, within ompW at 1312.413 kb on + strand, within ompW at 1312.413 kb on + strand, within ompW at 1312.480 kb on - strand, within ompW at 1312.480 kb on - strand, within ompW at 1312.496 kb on + strand, within ompW at 1312.496 kb on + strand, within ompW at 1312.507 kb on - strand, within ompW at 1312.517 kb on + strand, within ompW at 1312.564 kb on + strand, within ompW at 1312.566 kb on + strand, within ompW at 1312.571 kb on + strand, within ompW at 1312.571 kb on + strand, within ompW at 1312.574 kb on + strand, within ompW at 1312.597 kb on + strand, within ompW at 1312.609 kb on + strand, within ompW at 1312.612 kb on + strand, within ompW at 1312.612 kb on + strand, within ompW at 1312.612 kb on + strand, within ompW at 1312.635 kb on + strand at 1312.638 kb on - strand at 1312.642 kb on + strand at 1312.642 kb on + strand at 1312.642 kb on + strand at 1312.644 kb on + strand at 1312.644 kb on + strand at 1312.653 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 30 mM remove 1,310,036 + yciA b1253 0.41 -0.8 1,310,036 + yciA b1253 0.41 +0.8 1,310,046 + yciA b1253 0.44 +1.1 1,310,061 + yciA b1253 0.47 +0.2 1,310,141 - yciA b1253 0.67 +1.0 1,310,410 + +1.2 1,310,416 - -0.8 1,310,650 - yciB b1254 0.51 -0.8 1,310,809 - yciB b1254 0.80 +0.4 1,311,105 + yciC b1255 0.22 +1.1 1,311,105 + yciC b1255 0.22 +0.9 1,311,187 - yciC b1255 0.33 -1.0 1,311,189 + yciC b1255 0.33 +2.5 1,311,212 - yciC b1255 0.36 +0.9 1,311,212 - yciC b1255 0.36 +0.6 1,311,329 - yciC b1255 0.52 +1.3 1,311,380 + yciC b1255 0.59 -0.7 1,311,448 - yciC b1255 0.68 +0.4 1,311,448 - yciC b1255 0.68 +1.3 1,311,621 - +0.2 1,311,661 + +1.2 1,311,670 - +1.2 1,311,670 - +0.6 1,311,670 - +0.8 1,311,671 - +0.4 1,311,677 + +1.2 1,311,691 + +1.0 1,311,691 - +1.4 1,311,714 + +1.0 1,311,747 - +0.8 1,311,747 - +0.3 1,311,747 - -0.6 1,311,772 - +0.2 1,311,858 - -0.6 1,311,973 + +1.8 1,311,973 + +0.9 1,312,034 - +0.3 1,312,096 - -0.5 1,312,146 + ompW b1256 0.16 +1.3 1,312,234 + ompW b1256 0.30 +0.8 1,312,234 + ompW b1256 0.30 +0.1 1,312,237 - ompW b1256 0.30 +0.2 1,312,248 + ompW b1256 0.32 -0.2 1,312,369 + ompW b1256 0.51 +0.8 1,312,369 + ompW b1256 0.51 -0.1 1,312,372 - ompW b1256 0.51 +0.4 1,312,387 + ompW b1256 0.54 +1.1 1,312,413 + ompW b1256 0.58 -0.4 1,312,413 + ompW b1256 0.58 -0.2 1,312,480 - ompW b1256 0.68 +0.4 1,312,480 - ompW b1256 0.68 +0.5 1,312,496 + ompW b1256 0.71 +0.5 1,312,496 + ompW b1256 0.71 +0.2 1,312,507 - ompW b1256 0.72 -0.7 1,312,517 + ompW b1256 0.74 +0.3 1,312,564 + ompW b1256 0.81 +0.4 1,312,566 + ompW b1256 0.82 +0.1 1,312,571 + ompW b1256 0.82 +0.5 1,312,571 + ompW b1256 0.82 -1.8 1,312,574 + ompW b1256 0.83 +0.8 1,312,597 + ompW b1256 0.87 +0.6 1,312,609 + ompW b1256 0.88 -0.7 1,312,612 + ompW b1256 0.89 +2.8 1,312,612 + ompW b1256 0.89 +1.2 1,312,612 + ompW b1256 0.89 -0.5 1,312,635 + +1.2 1,312,638 - -0.6 1,312,642 + +0.8 1,312,642 + +0.2 1,312,642 + +0.3 1,312,644 + -1.6 1,312,644 + -1.1 1,312,653 + +0.8
Or see this region's nucleotide sequence