Experiment: 1-ethyl-3-methylimidazolium acetate 30 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycdW and ycdX are separated by 54 nucleotides ycdX and ycdY are separated by 23 nucleotides ycdY and ycdZ are separated by 101 nucleotides ycdZ and csgG are separated by 63 nucleotides
b1033: ycdW - putative dehydrogenase (VIMSS), at 1,097,109 to 1,098,047
ycdW
b1034: ycdX - hypothetical protein (NCBI), at 1,098,102 to 1,098,839
ycdX
b1035: ycdY - hypothetical protein (NCBI), at 1,098,863 to 1,099,417
ycdY
b1036: ycdZ - orf, hypothetical protein (VIMSS), at 1,099,519 to 1,100,010
ycdZ
b1037: csgG - outer membrane lipoprotein (NCBI), at 1,100,074 to 1,100,907
csgG
Position (kb)
1098
1099
1100 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 1097.934 kb on - strand, within ycdW at 1097.941 kb on + strand, within ycdW at 1098.011 kb on + strand at 1098.107 kb on - strand at 1098.107 kb on - strand at 1098.140 kb on + strand at 1098.174 kb on + strand at 1098.250 kb on + strand, within ycdX at 1098.274 kb on - strand, within ycdX at 1098.295 kb on + strand, within ycdX at 1098.303 kb on - strand, within ycdX at 1098.304 kb on - strand, within ycdX at 1098.304 kb on - strand, within ycdX at 1098.390 kb on - strand, within ycdX at 1098.390 kb on - strand, within ycdX at 1098.447 kb on - strand, within ycdX at 1098.459 kb on + strand, within ycdX at 1098.465 kb on - strand, within ycdX at 1098.465 kb on - strand, within ycdX at 1098.656 kb on - strand, within ycdX at 1098.800 kb on + strand at 1098.805 kb on + strand at 1098.805 kb on + strand at 1098.810 kb on + strand at 1098.813 kb on - strand at 1098.813 kb on - strand at 1098.826 kb on + strand at 1098.888 kb on - strand at 1098.892 kb on + strand at 1098.924 kb on + strand, within ycdY at 1098.998 kb on + strand, within ycdY at 1099.046 kb on + strand, within ycdY at 1099.106 kb on - strand, within ycdY at 1099.106 kb on - strand, within ycdY at 1099.189 kb on + strand, within ycdY at 1099.270 kb on - strand, within ycdY at 1099.290 kb on + strand, within ycdY at 1099.293 kb on - strand, within ycdY at 1099.293 kb on - strand, within ycdY at 1099.313 kb on + strand, within ycdY at 1099.313 kb on + strand, within ycdY at 1099.355 kb on - strand, within ycdY at 1099.588 kb on + strand, within ycdZ at 1099.623 kb on - strand, within ycdZ at 1099.702 kb on + strand, within ycdZ at 1099.702 kb on + strand, within ycdZ at 1099.710 kb on - strand, within ycdZ at 1099.727 kb on + strand, within ycdZ at 1099.758 kb on + strand, within ycdZ at 1099.812 kb on - strand, within ycdZ at 1099.880 kb on - strand, within ycdZ at 1099.962 kb on + strand at 1099.987 kb on - strand at 1099.987 kb on - strand at 1100.096 kb on - strand at 1100.152 kb on + strand at 1100.152 kb on + strand at 1100.172 kb on - strand, within csgG at 1100.172 kb on - strand, within csgG at 1100.172 kb on - strand, within csgG at 1100.221 kb on - strand, within csgG at 1100.223 kb on + strand, within csgG at 1100.223 kb on + strand, within csgG at 1100.235 kb on + strand, within csgG at 1100.249 kb on - strand, within csgG at 1100.251 kb on - strand, within csgG at 1100.265 kb on - strand, within csgG at 1100.363 kb on - strand, within csgG at 1100.410 kb on - strand, within csgG at 1100.410 kb on - strand, within csgG
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 30 mM remove 1,097,934 - ycdW b1033 0.88 +0.4 1,097,941 + ycdW b1033 0.89 +0.3 1,098,011 + +1.3 1,098,107 - -0.5 1,098,107 - -1.7 1,098,140 + +1.5 1,098,174 + +1.4 1,098,250 + ycdX b1034 0.20 +0.7 1,098,274 - ycdX b1034 0.23 -1.0 1,098,295 + ycdX b1034 0.26 +0.5 1,098,303 - ycdX b1034 0.27 -0.7 1,098,304 - ycdX b1034 0.27 +0.1 1,098,304 - ycdX b1034 0.27 +0.8 1,098,390 - ycdX b1034 0.39 -0.4 1,098,390 - ycdX b1034 0.39 -0.2 1,098,447 - ycdX b1034 0.47 +0.3 1,098,459 + ycdX b1034 0.48 +0.2 1,098,465 - ycdX b1034 0.49 -0.6 1,098,465 - ycdX b1034 0.49 +0.1 1,098,656 - ycdX b1034 0.75 +0.8 1,098,800 + +1.6 1,098,805 + +0.5 1,098,805 + +1.4 1,098,810 + +1.2 1,098,813 - +0.5 1,098,813 - +2.6 1,098,826 + +0.5 1,098,888 - +1.4 1,098,892 + +0.0 1,098,924 + ycdY b1035 0.11 +1.2 1,098,998 + ycdY b1035 0.24 +0.6 1,099,046 + ycdY b1035 0.33 +0.8 1,099,106 - ycdY b1035 0.44 +0.2 1,099,106 - ycdY b1035 0.44 -0.3 1,099,189 + ycdY b1035 0.59 +0.8 1,099,270 - ycdY b1035 0.73 -0.5 1,099,290 + ycdY b1035 0.77 +1.5 1,099,293 - ycdY b1035 0.77 +0.8 1,099,293 - ycdY b1035 0.77 -0.9 1,099,313 + ycdY b1035 0.81 +1.7 1,099,313 + ycdY b1035 0.81 +0.1 1,099,355 - ycdY b1035 0.89 +0.6 1,099,588 + ycdZ b1036 0.14 +2.7 1,099,623 - ycdZ b1036 0.21 +4.5 1,099,702 + ycdZ b1036 0.37 +2.7 1,099,702 + ycdZ b1036 0.37 +2.4 1,099,710 - ycdZ b1036 0.39 +2.9 1,099,727 + ycdZ b1036 0.42 +1.4 1,099,758 + ycdZ b1036 0.49 +3.1 1,099,812 - ycdZ b1036 0.60 +2.4 1,099,880 - ycdZ b1036 0.73 +3.1 1,099,962 + +2.4 1,099,987 - +2.7 1,099,987 - +3.2 1,100,096 - -0.3 1,100,152 + -0.7 1,100,152 + -0.4 1,100,172 - csgG b1037 0.12 -0.3 1,100,172 - csgG b1037 0.12 +0.2 1,100,172 - csgG b1037 0.12 -0.1 1,100,221 - csgG b1037 0.18 +1.2 1,100,223 + csgG b1037 0.18 -0.1 1,100,223 + csgG b1037 0.18 -0.7 1,100,235 + csgG b1037 0.19 +0.6 1,100,249 - csgG b1037 0.21 -0.0 1,100,251 - csgG b1037 0.21 +0.2 1,100,265 - csgG b1037 0.23 +0.1 1,100,363 - csgG b1037 0.35 +0.6 1,100,410 - csgG b1037 0.40 -0.1 1,100,410 - csgG b1037 0.40 +2.3
Or see this region's nucleotide sequence