Experiment: 1-ethyl-3-methylimidazolium acetate 30 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt moeA and iaaA are separated by 203 nucleotides iaaA and gsiA overlap by 14 nucleotides
b0827: moeA - molybdopterin biosynthesis protein (NCBI), at 864,352 to 865,587
moeA
b0828: iaaA - L-asparaginase (NCBI), at 865,791 to 866,756
iaaA
b0829: gsiA - fused predicted peptide transport subunits of ABC superfamily: ATP-binding components (RefSeq), at 866,743 to 868,614
gsiA
Position (kb) 865
866
867 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 864.814 kb on - strand, within moeA at 864.855 kb on + strand, within moeA at 864.945 kb on + strand, within moeA at 865.047 kb on + strand, within moeA at 865.064 kb on - strand, within moeA at 865.220 kb on + strand, within moeA at 865.243 kb on + strand, within moeA at 865.243 kb on + strand, within moeA at 865.278 kb on - strand, within moeA at 865.278 kb on - strand at 865.301 kb on - strand, within moeA at 865.527 kb on + strand at 865.527 kb on + strand at 865.535 kb on - strand at 865.561 kb on - strand at 865.646 kb on + strand at 865.646 kb on + strand at 865.648 kb on + strand at 865.771 kb on + strand at 865.771 kb on + strand at 865.875 kb on + strand at 865.883 kb on + strand at 866.064 kb on - strand, within iaaA at 866.149 kb on + strand, within iaaA at 866.246 kb on + strand at 866.424 kb on + strand, within iaaA at 866.432 kb on - strand, within iaaA at 866.537 kb on + strand, within iaaA at 866.954 kb on + strand, within gsiA at 866.954 kb on + strand, within gsiA at 867.026 kb on + strand, within gsiA at 867.026 kb on + strand, within gsiA at 867.041 kb on - strand, within gsiA at 867.041 kb on - strand, within gsiA at 867.102 kb on + strand, within gsiA at 867.102 kb on + strand, within gsiA at 867.206 kb on + strand, within gsiA at 867.206 kb on + strand, within gsiA at 867.297 kb on + strand, within gsiA at 867.468 kb on + strand, within gsiA at 867.537 kb on - strand, within gsiA at 867.559 kb on - strand, within gsiA at 867.591 kb on + strand, within gsiA at 867.591 kb on + strand, within gsiA at 867.697 kb on + strand, within gsiA at 867.724 kb on + strand, within gsiA at 867.753 kb on - strand, within gsiA
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 30 mM remove 864,814 - moeA b0827 0.37 +0.3 864,855 + moeA b0827 0.41 +0.1 864,945 + moeA b0827 0.48 +0.1 865,047 + moeA b0827 0.56 -0.1 865,064 - moeA b0827 0.58 -0.5 865,220 + moeA b0827 0.70 +0.6 865,243 + moeA b0827 0.72 +0.3 865,243 + moeA b0827 0.72 -0.1 865,278 - moeA b0827 0.75 +1.3 865,278 - +1.9 865,301 - moeA b0827 0.77 +0.9 865,527 + +0.1 865,527 + +1.1 865,535 - +1.1 865,561 - +0.6 865,646 + +0.0 865,646 + +0.7 865,648 + -0.1 865,771 + -2.0 865,771 + +0.3 865,875 + +0.3 865,883 + +0.2 866,064 - iaaA b0828 0.28 -0.1 866,149 + iaaA b0828 0.37 +1.4 866,246 + -0.1 866,424 + iaaA b0828 0.66 +0.4 866,432 - iaaA b0828 0.66 +0.3 866,537 + iaaA b0828 0.77 +1.0 866,954 + gsiA b0829 0.11 -1.3 866,954 + gsiA b0829 0.11 +0.6 867,026 + gsiA b0829 0.15 -0.1 867,026 + gsiA b0829 0.15 -0.1 867,041 - gsiA b0829 0.16 +0.9 867,041 - gsiA b0829 0.16 -0.7 867,102 + gsiA b0829 0.19 +0.9 867,102 + gsiA b0829 0.19 +0.9 867,206 + gsiA b0829 0.25 +1.6 867,206 + gsiA b0829 0.25 +0.9 867,297 + gsiA b0829 0.30 +0.0 867,468 + gsiA b0829 0.39 +2.9 867,537 - gsiA b0829 0.42 +1.2 867,559 - gsiA b0829 0.44 +0.6 867,591 + gsiA b0829 0.45 +0.9 867,591 + gsiA b0829 0.45 -1.1 867,697 + gsiA b0829 0.51 +0.9 867,724 + gsiA b0829 0.52 -0.3 867,753 - gsiA b0829 0.54 +0.9
Or see this region's nucleotide sequence