Experiment: 1-ethyl-3-methylimidazolium acetate 30 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt kdpA and kdpF overlap by 1 nucleotides kdpF and ybfA are separated by 312 nucleotides ybfA and rhsC are separated by 242 nucleotides rhsC and ybfB overlap by 1 nucleotides ybfB and ybfO are separated by 30 nucleotides
b0698: kdpA - potassium-transporting ATPase subunit A (NCBI), at 726,282 to 727,955
kdpA
b4513: kdpF - potassium ion accessory transporter subunit (NCBI), at 727,955 to 728,044
kdpF
b0699: ybfA - hypothetical protein (NCBI), at 728,357 to 728,563
ybfA
b0700: rhsC - rhsC element core protein RshC (NCBI), at 728,806 to 732,999
rhsC
b0702: ybfB - predicted inner membrane protein (NCBI), at 732,999 to 733,325
ybfB
b0703: ybfO - conserved protein, rhs-like (RefSeq), at 733,356 to 734,876
ybfO
Position (kb) 728
729
730
731
732
733 Strain fitness (log2 ratio) -1
0
1
2 at 727.871 kb on + strand at 727.871 kb on + strand at 727.884 kb on + strand at 727.904 kb on - strand at 727.904 kb on - strand at 727.963 kb on + strand at 727.966 kb on - strand at 727.988 kb on - strand, within kdpF at 728.022 kb on - strand, within kdpF at 728.054 kb on - strand at 728.054 kb on - strand at 728.059 kb on - strand at 728.068 kb on - strand at 728.076 kb on - strand at 728.580 kb on + strand at 728.597 kb on + strand at 728.641 kb on + strand at 728.641 kb on + strand at 728.758 kb on - strand at 728.810 kb on - strand at 728.840 kb on - strand at 728.863 kb on - strand at 729.778 kb on + strand, within rhsC at 729.786 kb on - strand, within rhsC at 730.011 kb on + strand, within rhsC at 730.178 kb on - strand, within rhsC at 730.178 kb on - strand, within rhsC at 730.222 kb on + strand, within rhsC at 730.222 kb on + strand, within rhsC at 730.659 kb on - strand, within rhsC at 730.998 kb on + strand, within rhsC at 730.998 kb on + strand, within rhsC at 731.001 kb on + strand, within rhsC at 731.031 kb on - strand, within rhsC at 731.505 kb on + strand, within rhsC at 732.219 kb on - strand, within rhsC at 732.248 kb on - strand, within rhsC at 732.288 kb on + strand, within rhsC at 732.288 kb on + strand, within rhsC at 732.298 kb on - strand, within rhsC at 732.306 kb on - strand, within rhsC at 732.560 kb on + strand, within rhsC at 732.560 kb on + strand, within rhsC at 732.561 kb on + strand, within rhsC at 732.588 kb on - strand at 732.605 kb on + strand at 732.610 kb on + strand at 732.613 kb on + strand at 732.627 kb on + strand at 732.649 kb on + strand at 732.667 kb on + strand at 732.669 kb on + strand at 732.671 kb on + strand at 732.672 kb on + strand at 732.672 kb on + strand at 732.672 kb on + strand at 732.672 kb on + strand at 732.672 kb on + strand at 732.672 kb on + strand at 732.672 kb on + strand at 732.672 kb on + strand at 732.702 kb on + strand at 732.702 kb on + strand at 732.716 kb on + strand at 732.716 kb on + strand at 732.716 kb on + strand at 732.716 kb on + strand at 732.716 kb on + strand at 732.716 kb on + strand at 732.716 kb on + strand at 732.724 kb on - strand at 732.729 kb on + strand at 732.730 kb on + strand at 732.730 kb on + strand at 732.739 kb on + strand at 732.766 kb on + strand at 732.766 kb on + strand at 732.792 kb on + strand at 732.809 kb on + strand at 732.821 kb on + strand at 732.828 kb on + strand at 732.830 kb on + strand at 732.831 kb on + strand at 732.840 kb on + strand at 733.366 kb on - strand at 733.374 kb on - strand at 733.381 kb on - strand at 733.381 kb on - strand at 733.382 kb on - strand at 733.382 kb on - strand at 733.384 kb on - strand at 733.400 kb on - strand at 733.400 kb on - strand at 733.400 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 30 mM remove 727,871 + -0.3 727,871 + +0.5 727,884 + -0.2 727,904 - +0.0 727,904 - +0.5 727,963 + -0.3 727,966 - +0.9 727,988 - kdpF b4513 0.37 +0.7 728,022 - kdpF b4513 0.74 +0.4 728,054 - -0.3 728,054 - -0.3 728,059 - +1.3 728,068 - +0.1 728,076 - -0.1 728,580 + +0.5 728,597 + +1.0 728,641 + +0.8 728,641 + +0.2 728,758 - +0.9 728,810 - -0.7 728,840 - -0.7 728,863 - -0.7 729,778 + rhsC b0700 0.23 +0.1 729,786 - rhsC b0700 0.23 +0.2 730,011 + rhsC b0700 0.29 -1.2 730,178 - rhsC b0700 0.33 +0.3 730,178 - rhsC b0700 0.33 -1.2 730,222 + rhsC b0700 0.34 -0.8 730,222 + rhsC b0700 0.34 +0.6 730,659 - rhsC b0700 0.44 +0.5 730,998 + rhsC b0700 0.52 +0.9 730,998 + rhsC b0700 0.52 +0.3 731,001 + rhsC b0700 0.52 -0.7 731,031 - rhsC b0700 0.53 -0.4 731,505 + rhsC b0700 0.64 -0.0 732,219 - rhsC b0700 0.81 -0.4 732,248 - rhsC b0700 0.82 -1.3 732,288 + rhsC b0700 0.83 -0.1 732,288 + rhsC b0700 0.83 +0.9 732,298 - rhsC b0700 0.83 -0.0 732,306 - rhsC b0700 0.83 +0.9 732,560 + rhsC b0700 0.90 -1.0 732,560 + rhsC b0700 0.90 -0.5 732,561 + rhsC b0700 0.90 -1.0 732,588 - +0.1 732,605 + +1.9 732,610 + +0.1 732,613 + -0.6 732,627 + +0.9 732,649 + -0.3 732,667 + +0.2 732,669 + -0.2 732,671 + +0.3 732,672 + +0.2 732,672 + +1.2 732,672 + -0.5 732,672 + -0.0 732,672 + -0.5 732,672 + -1.4 732,672 + -0.3 732,672 + +1.1 732,702 + -0.3 732,702 + -0.3 732,716 + +1.9 732,716 + +0.6 732,716 + +1.1 732,716 + -0.4 732,716 + -0.2 732,716 + -1.1 732,716 + -0.1 732,724 - -1.3 732,729 + +0.0 732,730 + +0.9 732,730 + -1.2 732,739 + +1.0 732,766 + +0.4 732,766 + -1.1 732,792 + +0.9 732,809 + -1.0 732,821 + +0.1 732,828 + -0.5 732,830 + +0.3 732,831 + +0.2 732,840 + +0.3 733,366 - -0.4 733,374 - -1.0 733,381 - -1.4 733,381 - +0.2 733,382 - -0.3 733,382 - +0.4 733,384 - -0.2 733,400 - +1.6 733,400 - +1.9 733,400 - -0.4
Or see this region's nucleotide sequence