Experiment: 1-ethyl-3-methylimidazolium acetate 30 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt insF-3 and emrE are separated by 310 nucleotides emrE and ybcK are separated by 254 nucleotides
b0541: insF-3 - DLP12 prophage; IS3 element protein InsF (NCBI), at 566,361 to 567,227
insF-3
b0543: emrE - multidrug efflux protein (NCBI), at 567,538 to 567,870
emrE
b0544: ybcK - DLP12 prophage; predicted recombinase (NCBI), at 568,125 to 569,651
ybcK
Position (kb) 567
568 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 567.210 kb on + strand at 567.210 kb on + strand at 567.221 kb on + strand at 567.221 kb on + strand at 567.222 kb on + strand at 567.222 kb on + strand at 567.262 kb on + strand at 567.275 kb on - strand at 567.275 kb on - strand at 567.320 kb on + strand at 567.323 kb on + strand at 567.323 kb on + strand at 567.323 kb on + strand at 567.333 kb on + strand at 567.333 kb on + strand at 567.333 kb on + strand at 567.333 kb on + strand at 567.341 kb on - strand at 567.344 kb on + strand at 567.354 kb on + strand at 567.362 kb on - strand at 567.404 kb on + strand at 567.404 kb on + strand at 567.410 kb on + strand at 567.410 kb on + strand at 567.438 kb on + strand at 567.463 kb on + strand at 567.467 kb on + strand at 567.467 kb on + strand at 567.468 kb on + strand at 567.468 kb on + strand at 567.478 kb on + strand at 567.481 kb on + strand at 567.481 kb on + strand at 567.483 kb on + strand at 567.487 kb on + strand at 567.524 kb on + strand at 567.524 kb on + strand at 567.543 kb on + strand at 567.543 kb on + strand at 567.563 kb on + strand at 567.569 kb on + strand at 567.577 kb on + strand, within emrE at 567.579 kb on + strand, within emrE at 567.582 kb on + strand, within emrE at 567.584 kb on + strand, within emrE at 567.585 kb on + strand, within emrE at 567.592 kb on + strand, within emrE at 567.603 kb on + strand, within emrE at 567.605 kb on + strand, within emrE at 567.605 kb on + strand, within emrE at 567.606 kb on + strand, within emrE at 567.606 kb on + strand, within emrE at 567.606 kb on + strand, within emrE at 567.606 kb on + strand, within emrE at 567.624 kb on + strand, within emrE at 567.624 kb on + strand, within emrE at 567.664 kb on + strand, within emrE at 567.671 kb on + strand, within emrE at 567.671 kb on + strand, within emrE at 567.679 kb on + strand, within emrE at 567.679 kb on + strand, within emrE at 567.692 kb on + strand, within emrE at 567.698 kb on + strand, within emrE at 567.711 kb on + strand, within emrE at 567.711 kb on + strand, within emrE at 567.711 kb on + strand, within emrE at 567.711 kb on + strand, within emrE at 567.712 kb on + strand, within emrE at 567.712 kb on + strand, within emrE at 567.713 kb on + strand, within emrE at 567.715 kb on + strand, within emrE at 567.720 kb on + strand, within emrE at 567.730 kb on + strand, within emrE at 567.737 kb on + strand, within emrE at 567.737 kb on + strand, within emrE at 567.742 kb on + strand, within emrE at 567.742 kb on + strand, within emrE at 567.742 kb on + strand, within emrE at 567.742 kb on + strand, within emrE at 567.745 kb on + strand, within emrE at 567.745 kb on + strand, within emrE at 567.751 kb on + strand, within emrE at 567.751 kb on + strand, within emrE at 567.761 kb on + strand, within emrE at 567.761 kb on + strand, within emrE at 567.782 kb on + strand, within emrE at 567.796 kb on + strand, within emrE at 567.796 kb on + strand, within emrE at 567.797 kb on + strand, within emrE at 567.797 kb on + strand, within emrE at 567.800 kb on + strand, within emrE at 567.811 kb on + strand, within emrE at 567.811 kb on + strand, within emrE at 567.811 kb on + strand, within emrE at 567.811 kb on + strand, within emrE at 567.813 kb on + strand, within emrE at 567.813 kb on + strand, within emrE at 568.692 kb on + strand, within ybcK
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 30 mM remove 567,210 + +0.9 567,210 + +0.5 567,221 + +0.5 567,221 + +0.6 567,222 + +0.4 567,222 + +0.7 567,262 + +0.4 567,275 - -0.9 567,275 - -2.4 567,320 + +1.0 567,323 + +1.5 567,323 + +1.9 567,323 + +1.2 567,333 + +1.3 567,333 + +0.9 567,333 + +0.9 567,333 + +0.0 567,341 - -3.2 567,344 + +0.7 567,354 + +2.8 567,362 - -2.2 567,404 + +0.1 567,404 + +0.5 567,410 + +1.4 567,410 + -0.1 567,438 + +0.5 567,463 + +4.5 567,467 + +2.7 567,467 + +2.4 567,468 + +2.7 567,468 + +2.7 567,478 + +0.5 567,481 + +0.9 567,481 + +2.9 567,483 + +3.0 567,487 + +1.4 567,524 + +2.7 567,524 + +2.2 567,543 + +0.9 567,543 + -0.7 567,563 + -0.9 567,569 + -2.2 567,577 + emrE b0543 0.12 -1.1 567,579 + emrE b0543 0.12 -1.5 567,582 + emrE b0543 0.13 -1.8 567,584 + emrE b0543 0.14 -1.1 567,585 + emrE b0543 0.14 -2.9 567,592 + emrE b0543 0.16 -3.0 567,603 + emrE b0543 0.20 -2.5 567,605 + emrE b0543 0.20 -1.4 567,605 + emrE b0543 0.20 -0.1 567,606 + emrE b0543 0.20 -1.7 567,606 + emrE b0543 0.20 -2.4 567,606 + emrE b0543 0.20 -0.7 567,606 + emrE b0543 0.20 -1.7 567,624 + emrE b0543 0.26 -2.7 567,624 + emrE b0543 0.26 -0.5 567,664 + emrE b0543 0.38 -2.2 567,671 + emrE b0543 0.40 -0.1 567,671 + emrE b0543 0.40 -2.5 567,679 + emrE b0543 0.42 -2.5 567,679 + emrE b0543 0.42 -1.7 567,692 + emrE b0543 0.46 -3.5 567,698 + emrE b0543 0.48 +0.9 567,711 + emrE b0543 0.52 -2.1 567,711 + emrE b0543 0.52 -1.5 567,711 + emrE b0543 0.52 -1.4 567,711 + emrE b0543 0.52 -0.7 567,712 + emrE b0543 0.52 -1.4 567,712 + emrE b0543 0.52 -1.4 567,713 + emrE b0543 0.53 -1.1 567,715 + emrE b0543 0.53 -1.1 567,720 + emrE b0543 0.55 -2.5 567,730 + emrE b0543 0.58 -2.7 567,737 + emrE b0543 0.60 -2.8 567,737 + emrE b0543 0.60 -2.0 567,742 + emrE b0543 0.61 -3.1 567,742 + emrE b0543 0.61 -2.4 567,742 + emrE b0543 0.61 -0.5 567,742 + emrE b0543 0.61 -1.9 567,745 + emrE b0543 0.62 -3.2 567,745 + emrE b0543 0.62 -1.4 567,751 + emrE b0543 0.64 -2.1 567,751 + emrE b0543 0.64 +0.9 567,761 + emrE b0543 0.67 +0.9 567,761 + emrE b0543 0.67 -1.4 567,782 + emrE b0543 0.73 -2.1 567,796 + emrE b0543 0.77 -2.8 567,796 + emrE b0543 0.77 -2.4 567,797 + emrE b0543 0.78 -2.3 567,797 + emrE b0543 0.78 -2.7 567,800 + emrE b0543 0.79 -2.4 567,811 + emrE b0543 0.82 -0.7 567,811 + emrE b0543 0.82 -1.7 567,811 + emrE b0543 0.82 -1.4 567,811 + emrE b0543 0.82 -2.1 567,813 + emrE b0543 0.83 -0.1 567,813 + emrE b0543 0.83 -1.9 568,692 + ybcK b0544 0.37 -1.2
Or see this region's nucleotide sequence