Strain Fitness in Escherichia coli BW25113 around b0044

Experiment: 1-ethyl-3-methylimidazolium acetate 30 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfixC and fixX overlap by 4 nucleotidesfixX and yaaU are separated by 56 nucleotides b0043: fixC - predicted oxidoreductase with FAD/NAD(P)-binding domain (NCBI), at 44,180 to 45,466 fixC b0044: fixX - predicted 4Fe-4S ferredoxin-type protein (NCBI), at 45,463 to 45,750 fixX b0045: yaaU - predicted transporter (NCBI), at 45,807 to 47,138 yaaU Position (kb) 45 46Strain fitness (log2 ratio) -2 -1 0 1at 44.581 kb on - strand, within fixCat 44.622 kb on - strand, within fixCat 44.622 kb on - strand, within fixCat 44.649 kb on + strand, within fixCat 44.675 kb on - strand, within fixCat 44.676 kb on - strand, within fixCat 44.765 kb on + strand, within fixCat 44.857 kb on - strand, within fixCat 44.862 kb on - strand, within fixCat 44.906 kb on - strand, within fixCat 45.034 kb on + strand, within fixCat 45.034 kb on + strand, within fixCat 45.044 kb on + strand, within fixCat 45.044 kb on + strand, within fixCat 45.232 kb on + strand, within fixCat 45.241 kb on - strand, within fixCat 45.323 kb on - strand, within fixCat 45.339 kb on + strandat 45.339 kb on + strandat 45.340 kb on - strandat 45.426 kb on + strandat 45.546 kb on - strand, within fixXat 45.610 kb on + strand, within fixXat 45.700 kb on - strand, within fixXat 45.704 kb on + strand, within fixXat 45.704 kb on + strand, within fixXat 45.844 kb on + strandat 45.880 kb on - strandat 45.924 kb on + strandat 46.113 kb on + strand, within yaaUat 46.113 kb on + strand, within yaaUat 46.142 kb on - strand, within yaaUat 46.144 kb on - strand, within yaaUat 46.176 kb on - strand, within yaaUat 46.176 kb on - strand, within yaaUat 46.240 kb on + strand, within yaaUat 46.284 kb on - strand, within yaaUat 46.329 kb on + strand, within yaaUat 46.329 kb on + strand, within yaaUat 46.463 kb on + strand, within yaaUat 46.463 kb on + strand, within yaaUat 46.564 kb on + strand, within yaaUat 46.564 kb on + strand, within yaaUat 46.575 kb on + strand, within yaaUat 46.707 kb on - strand, within yaaUat 46.707 kb on - strand, within yaaU

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 30 mM
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44,581 - fixC b0043 0.31 -0.4
44,622 - fixC b0043 0.34 -0.2
44,622 - fixC b0043 0.34 +0.0
44,649 + fixC b0043 0.36 +1.2
44,675 - fixC b0043 0.38 +0.3
44,676 - fixC b0043 0.39 +0.2
44,765 + fixC b0043 0.45 -0.1
44,857 - fixC b0043 0.53 +1.3
44,862 - fixC b0043 0.53 +0.1
44,906 - fixC b0043 0.56 -0.8
45,034 + fixC b0043 0.66 +0.3
45,034 + fixC b0043 0.66 -1.8
45,044 + fixC b0043 0.67 -0.6
45,044 + fixC b0043 0.67 -0.1
45,232 + fixC b0043 0.82 +1.0
45,241 - fixC b0043 0.82 +0.3
45,323 - fixC b0043 0.89 -0.3
45,339 + +1.0
45,339 + +1.6
45,340 - +0.1
45,426 + +0.3
45,546 - fixX b0044 0.29 +1.1
45,610 + fixX b0044 0.51 +0.4
45,700 - fixX b0044 0.82 +1.2
45,704 + fixX b0044 0.84 +0.5
45,704 + fixX b0044 0.84 +0.4
45,844 + +0.4
45,880 - +0.4
45,924 + +0.3
46,113 + yaaU b0045 0.23 +0.1
46,113 + yaaU b0045 0.23 +0.4
46,142 - yaaU b0045 0.25 -0.1
46,144 - yaaU b0045 0.25 +0.4
46,176 - yaaU b0045 0.28 +1.0
46,176 - yaaU b0045 0.28 +0.3
46,240 + yaaU b0045 0.33 +0.2
46,284 - yaaU b0045 0.36 -0.4
46,329 + yaaU b0045 0.39 -1.4
46,329 + yaaU b0045 0.39 -0.2
46,463 + yaaU b0045 0.49 +0.4
46,463 + yaaU b0045 0.49 +0.0
46,564 + yaaU b0045 0.57 +0.2
46,564 + yaaU b0045 0.57 +0.0
46,575 + yaaU b0045 0.58 +0.0
46,707 - yaaU b0045 0.68 +1.0
46,707 - yaaU b0045 0.68 -0.3

Or see this region's nucleotide sequence