Experiment: Gly-Glu (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_18585 and PS417_18590 are separated by 53 nucleotides PS417_18590 and PS417_18595 are separated by 162 nucleotides PS417_18595 and PS417_18600 are separated by 10 nucleotides
PS417_18585: PS417_18585 - 3-isopropylmalate dehydrogenase, at 4,036,729 to 4,037,811
_18585
PS417_18590: PS417_18590 - SAM-dependent methyltransferase, at 4,037,865 to 4,038,629
_18590
PS417_18595: PS417_18595 - 3-isopropylmalate dehydratase, at 4,038,792 to 4,039,436
_18595
PS417_18600: PS417_18600 - isopropylmalate isomerase, at 4,039,447 to 4,040,865
_18600
Position (kb) 4038
4039
4040 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 4037.889 kb on + strand at 4037.889 kb on + strand at 4038.022 kb on + strand, within PS417_18590 at 4038.022 kb on + strand, within PS417_18590 at 4038.050 kb on + strand, within PS417_18590 at 4038.154 kb on + strand, within PS417_18590 at 4038.154 kb on + strand, within PS417_18590 at 4038.155 kb on - strand, within PS417_18590 at 4038.155 kb on - strand, within PS417_18590 at 4038.783 kb on + strand at 4038.787 kb on + strand at 4038.937 kb on + strand, within PS417_18595 at 4039.102 kb on + strand, within PS417_18595 at 4039.102 kb on + strand, within PS417_18595 at 4039.279 kb on + strand, within PS417_18595 at 4039.878 kb on - strand, within PS417_18600 at 4039.878 kb on - strand, within PS417_18600
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-Glu (C) remove 4,037,889 + +0.0 4,037,889 + -0.3 4,038,022 + PS417_18590 0.21 -0.6 4,038,022 + PS417_18590 0.21 -0.3 4,038,050 + PS417_18590 0.24 -0.7 4,038,154 + PS417_18590 0.38 -1.3 4,038,154 + PS417_18590 0.38 -1.4 4,038,155 - PS417_18590 0.38 -0.3 4,038,155 - PS417_18590 0.38 +1.1 4,038,783 + -0.6 4,038,787 + -0.0 4,038,937 + PS417_18595 0.22 -1.4 4,039,102 + PS417_18595 0.48 -5.5 4,039,102 + PS417_18595 0.48 -1.9 4,039,279 + PS417_18595 0.76 -4.0 4,039,878 - PS417_18600 0.30 -4.3 4,039,878 - PS417_18600 0.30 -4.9
Or see this region's nucleotide sequence