Experiment: Gly-Glu (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_08535 and PS417_08540 are separated by 422 nucleotides PS417_08540 and PS417_08545 are separated by 96 nucleotides PS417_08545 and PS417_08550 are separated by 103 nucleotides
PS417_08535: PS417_08535 - enoyl-CoA hydratase, at 1,864,014 to 1,864,805
_08535
PS417_08540: PS417_08540 - RNA helicase, at 1,865,228 to 1,866,901
_08540
PS417_08545: PS417_08545 - aromatic ring-opening dioxygenase LigB, at 1,866,998 to 1,867,765
_08545
PS417_08550: PS417_08550 - thiopurine S-methyltransferase, at 1,867,869 to 1,868,525
_08550
Position (kb)
1865
1866
1867 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1864.746 kb on + strand at 1864.790 kb on + strand at 1864.871 kb on + strand at 1864.925 kb on - strand at 1864.925 kb on - strand at 1865.004 kb on + strand at 1865.004 kb on + strand at 1865.004 kb on + strand at 1865.036 kb on + strand at 1865.036 kb on + strand at 1865.215 kb on + strand at 1866.704 kb on - strand, within PS417_08540 at 1866.930 kb on - strand at 1866.930 kb on - strand at 1866.980 kb on - strand at 1867.012 kb on - strand at 1867.089 kb on + strand, within PS417_08545 at 1867.410 kb on + strand, within PS417_08545 at 1867.411 kb on - strand, within PS417_08545 at 1867.765 kb on + strand at 1867.765 kb on + strand at 1867.765 kb on + strand at 1867.766 kb on - strand at 1867.766 kb on - strand at 1867.766 kb on - strand at 1867.766 kb on - strand at 1867.825 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-Glu (C) remove 1,864,746 + -0.3 1,864,790 + -1.0 1,864,871 + +0.1 1,864,925 - -0.8 1,864,925 - -0.8 1,865,004 + -0.1 1,865,004 + -1.0 1,865,004 + +0.4 1,865,036 + -0.8 1,865,036 + -1.3 1,865,215 + -0.7 1,866,704 - PS417_08540 0.88 -2.3 1,866,930 - -0.2 1,866,930 - +2.0 1,866,980 - -0.2 1,867,012 - -0.2 1,867,089 + PS417_08545 0.12 +0.6 1,867,410 + PS417_08545 0.54 +1.0 1,867,411 - PS417_08545 0.54 -1.0 1,867,765 + -0.1 1,867,765 + -0.0 1,867,765 + +0.3 1,867,766 - -0.6 1,867,766 - -0.0 1,867,766 - +0.4 1,867,766 - -0.6 1,867,825 - +0.7
Or see this region's nucleotide sequence