Experiment: a-Cyclodextrin (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_0089 and Echvi_0090 are separated by 191 nucleotides Echvi_0090 and Echvi_0091 are separated by 324 nucleotides Echvi_0091 and Echvi_0092 are separated by 141 nucleotides
Echvi_0089: Echvi_0089 - methylmalonyl-CoA epimerase, at 89,002 to 89,406
_0089
Echvi_0090: Echvi_0090 - Iron-sulfur cluster assembly accessory protein, at 89,598 to 89,924
_0090
Echvi_0091: Echvi_0091 - glycine cleavage system T protein, at 90,249 to 91,343
_0091
Echvi_0092: Echvi_0092 - Phosphosulfolactate phosphohydrolase and related enzymes, at 91,485 to 92,198
_0092
Position (kb) 90
91
92 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 89.445 kb on + strand at 89.450 kb on + strand at 89.450 kb on + strand at 89.451 kb on - strand at 89.454 kb on + strand at 89.508 kb on + strand at 89.575 kb on + strand at 89.575 kb on + strand at 89.578 kb on + strand at 89.591 kb on - strand at 89.597 kb on + strand at 89.597 kb on + strand at 89.684 kb on - strand, within Echvi_0090 at 89.761 kb on + strand, within Echvi_0090 at 89.762 kb on - strand, within Echvi_0090 at 89.811 kb on + strand, within Echvi_0090 at 89.944 kb on - strand at 89.944 kb on - strand at 89.946 kb on - strand at 89.949 kb on - strand at 89.951 kb on + strand at 89.952 kb on - strand at 89.975 kb on - strand at 90.084 kb on + strand at 90.085 kb on - strand at 90.085 kb on - strand at 90.111 kb on + strand at 90.111 kb on + strand at 90.111 kb on + strand at 90.111 kb on + strand at 90.112 kb on - strand at 90.112 kb on - strand at 90.134 kb on - strand at 90.134 kb on - strand at 90.134 kb on - strand at 90.216 kb on - strand at 90.246 kb on + strand at 90.246 kb on + strand at 90.368 kb on + strand, within Echvi_0091 at 90.369 kb on - strand, within Echvi_0091 at 90.423 kb on - strand, within Echvi_0091 at 90.580 kb on - strand, within Echvi_0091 at 90.825 kb on - strand, within Echvi_0091 at 90.847 kb on + strand, within Echvi_0091 at 90.847 kb on + strand, within Echvi_0091 at 90.848 kb on - strand, within Echvi_0091 at 90.877 kb on + strand, within Echvi_0091 at 91.296 kb on - strand at 91.346 kb on + strand at 91.346 kb on + strand at 91.347 kb on - strand at 91.348 kb on + strand at 91.348 kb on + strand at 91.348 kb on + strand at 91.348 kb on + strand at 91.349 kb on - strand at 91.408 kb on - strand at 91.426 kb on + strand at 91.426 kb on + strand at 91.671 kb on + strand, within Echvi_0092 at 91.671 kb on + strand, within Echvi_0092 at 91.673 kb on + strand, within Echvi_0092 at 91.875 kb on + strand, within Echvi_0092 at 91.876 kb on - strand, within Echvi_0092 at 91.876 kb on - strand, within Echvi_0092 at 91.953 kb on + strand, within Echvi_0092 at 92.189 kb on - strand at 92.223 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Cyclodextrin (C) remove 89,445 + -1.0 89,450 + -1.9 89,450 + -0.2 89,451 - -0.5 89,454 + +0.0 89,508 + -0.8 89,575 + +0.1 89,575 + -0.3 89,578 + +0.7 89,591 - +0.1 89,597 + -0.2 89,597 + -2.8 89,684 - Echvi_0090 0.26 -0.9 89,761 + Echvi_0090 0.50 -0.7 89,762 - Echvi_0090 0.50 -0.2 89,811 + Echvi_0090 0.65 -0.9 89,944 - -0.1 89,944 - +0.3 89,946 - +0.3 89,949 - +0.3 89,951 + +0.2 89,952 - +0.1 89,975 - -0.4 90,084 + -0.1 90,085 - +0.3 90,085 - -0.2 90,111 + -0.2 90,111 + -0.3 90,111 + +0.7 90,111 + +0.6 90,112 - +1.0 90,112 - -0.9 90,134 - +0.1 90,134 - -1.8 90,134 - +1.1 90,216 - +0.6 90,246 + -1.1 90,246 + +0.2 90,368 + Echvi_0091 0.11 -0.8 90,369 - Echvi_0091 0.11 -0.9 90,423 - Echvi_0091 0.16 +0.5 90,580 - Echvi_0091 0.30 -0.9 90,825 - Echvi_0091 0.53 +0.5 90,847 + Echvi_0091 0.55 -2.2 90,847 + Echvi_0091 0.55 -0.5 90,848 - Echvi_0091 0.55 -0.6 90,877 + Echvi_0091 0.57 +0.4 91,296 - -1.3 91,346 + -0.2 91,346 + -0.1 91,347 - +1.0 91,348 + +0.4 91,348 + -0.6 91,348 + +0.3 91,348 + -0.9 91,349 - +1.7 91,408 - -0.6 91,426 + -0.5 91,426 + -0.3 91,671 + Echvi_0092 0.26 -3.6 91,671 + Echvi_0092 0.26 -3.0 91,673 + Echvi_0092 0.26 -1.1 91,875 + Echvi_0092 0.55 -1.3 91,876 - Echvi_0092 0.55 +0.1 91,876 - Echvi_0092 0.55 -3.1 91,953 + Echvi_0092 0.66 -1.9 92,189 - -3.2 92,223 - +1.7
Or see this region's nucleotide sequence