Strain Fitness in Escherichia coli BL21 around ECD_01276

Experiment: NoPhageControl

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01275 and ECD_01276 are separated by 26 nucleotidesECD_01276 and ECD_01277 are separated by 274 nucleotides ECD_01275: ECD_01275 - gamma-glutamyl-gamma-aminobutyrate hydrolase, at 1,347,486 to 1,348,250 _01275 ECD_01276: ECD_01276 - repressor for the divergent puu operons, putrescine inducible, at 1,348,277 to 1,348,834 _01276 ECD_01277: ECD_01277 - gamma-glutamyl-gamma-aminobutyraldehyde dehydrogenase; succinate semialdehyde dehydrogenase, at 1,349,109 to 1,350,596 _01277 Position (kb) 1348 1349Strain fitness (log2 ratio) -1 0 1 2 3at 1347.439 kb on + strandat 1347.718 kb on + strand, within ECD_01275at 1347.718 kb on + strand, within ECD_01275at 1347.718 kb on + strand, within ECD_01275at 1347.718 kb on + strand, within ECD_01275at 1348.030 kb on + strand, within ECD_01275at 1348.030 kb on + strand, within ECD_01275at 1348.096 kb on + strand, within ECD_01275at 1348.141 kb on + strand, within ECD_01275at 1348.189 kb on + strandat 1348.205 kb on + strandat 1348.451 kb on + strand, within ECD_01276at 1348.451 kb on + strand, within ECD_01276at 1348.451 kb on + strand, within ECD_01276at 1348.530 kb on + strand, within ECD_01276at 1348.668 kb on + strand, within ECD_01276at 1348.750 kb on + strand, within ECD_01276at 1348.750 kb on + strand, within ECD_01276at 1348.750 kb on + strand, within ECD_01276at 1348.816 kb on + strandat 1349.007 kb on + strandat 1349.036 kb on + strandat 1349.079 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction NoPhageControl
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1,347,439 + +1.6
1,347,718 + ECD_01275 0.30 -0.4
1,347,718 + ECD_01275 0.30 +1.0
1,347,718 + ECD_01275 0.30 +0.8
1,347,718 + ECD_01275 0.30 -0.3
1,348,030 + ECD_01275 0.71 -0.0
1,348,030 + ECD_01275 0.71 +1.7
1,348,096 + ECD_01275 0.80 -0.2
1,348,141 + ECD_01275 0.86 +2.1
1,348,189 + +1.0
1,348,205 + -0.2
1,348,451 + ECD_01276 0.31 +1.9
1,348,451 + ECD_01276 0.31 +1.5
1,348,451 + ECD_01276 0.31 +3.1
1,348,530 + ECD_01276 0.45 +1.1
1,348,668 + ECD_01276 0.70 +0.8
1,348,750 + ECD_01276 0.85 +1.8
1,348,750 + ECD_01276 0.85 +3.5
1,348,750 + ECD_01276 0.85 +2.5
1,348,816 + +0.7
1,349,007 + +0.8
1,349,036 + +1.6
1,349,079 - +1.1

Or see this region's nucleotide sequence