Strain Fitness in Escherichia coli BW25113 around b2546

Experiment: acetate 70 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyphC and yphD are separated by 65 nucleotidesyphD and yphE are separated by 24 nucleotides b2545: yphC - putative oxidoreductase (VIMSS), at 2,672,722 to 2,673,783 yphC b2546: yphD - predicted sugar transporter subunit: membrane component of ABC superfamily (NCBI), at 2,673,849 to 2,674,847 yphD b2547: yphE - fused predicted sugar transporter subunits of ABC superfamily: ATP-binding components (NCBI), at 2,674,872 to 2,676,383 yphE Position (kb) 2673 2674 2675Strain fitness (log2 ratio) -2 -1 0 1at 2672.870 kb on + strand, within yphCat 2672.906 kb on - strand, within yphCat 2672.908 kb on + strand, within yphCat 2672.988 kb on + strand, within yphCat 2673.134 kb on - strand, within yphCat 2673.165 kb on + strand, within yphCat 2673.173 kb on + strand, within yphCat 2673.173 kb on + strand, within yphCat 2673.231 kb on + strand, within yphCat 2673.231 kb on + strand, within yphCat 2673.289 kb on + strand, within yphCat 2673.578 kb on - strand, within yphCat 2673.578 kb on - strand, within yphCat 2673.578 kb on - strand, within yphCat 2673.693 kb on + strandat 2673.800 kb on - strandat 2673.959 kb on + strand, within yphDat 2674.037 kb on + strand, within yphDat 2674.303 kb on - strand, within yphDat 2674.305 kb on + strand, within yphDat 2674.308 kb on - strand, within yphDat 2674.350 kb on + strand, within yphDat 2674.350 kb on + strand, within yphDat 2674.350 kb on + strand, within yphDat 2674.358 kb on - strand, within yphDat 2674.358 kb on - strand, within yphDat 2674.358 kb on - strand, within yphDat 2674.381 kb on + strand, within yphDat 2674.457 kb on + strand, within yphDat 2674.457 kb on + strand, within yphDat 2674.466 kb on - strand, within yphDat 2674.466 kb on - strand, within yphDat 2674.476 kb on - strand, within yphDat 2674.532 kb on - strand, within yphDat 2674.836 kb on - strandat 2674.884 kb on - strandat 2674.904 kb on + strandat 2674.904 kb on + strandat 2675.002 kb on + strandat 2675.130 kb on - strand, within yphEat 2675.252 kb on - strand, within yphEat 2675.436 kb on - strand, within yphEat 2675.657 kb on + strand, within yphEat 2675.657 kb on + strand, within yphEat 2675.706 kb on - strand, within yphE

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Per-strain Table

Position Strand Gene LocusTag Fraction acetate 70 mM
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2,672,870 + yphC b2545 0.14 -0.7
2,672,906 - yphC b2545 0.17 -0.6
2,672,908 + yphC b2545 0.18 -0.1
2,672,988 + yphC b2545 0.25 +0.3
2,673,134 - yphC b2545 0.39 +0.3
2,673,165 + yphC b2545 0.42 -0.3
2,673,173 + yphC b2545 0.42 -1.4
2,673,173 + yphC b2545 0.42 -0.1
2,673,231 + yphC b2545 0.48 -0.7
2,673,231 + yphC b2545 0.48 +0.9
2,673,289 + yphC b2545 0.53 -0.4
2,673,578 - yphC b2545 0.81 -0.1
2,673,578 - yphC b2545 0.81 -2.3
2,673,578 - yphC b2545 0.81 +0.6
2,673,693 + -1.8
2,673,800 - +1.2
2,673,959 + yphD b2546 0.11 -0.7
2,674,037 + yphD b2546 0.19 -0.2
2,674,303 - yphD b2546 0.45 +0.8
2,674,305 + yphD b2546 0.46 -0.7
2,674,308 - yphD b2546 0.46 +0.2
2,674,350 + yphD b2546 0.50 +1.5
2,674,350 + yphD b2546 0.50 +0.6
2,674,350 + yphD b2546 0.50 +0.9
2,674,358 - yphD b2546 0.51 +0.5
2,674,358 - yphD b2546 0.51 +1.0
2,674,358 - yphD b2546 0.51 -0.5
2,674,381 + yphD b2546 0.53 +0.6
2,674,457 + yphD b2546 0.61 +1.1
2,674,457 + yphD b2546 0.61 +0.9
2,674,466 - yphD b2546 0.62 -0.3
2,674,466 - yphD b2546 0.62 +0.3
2,674,476 - yphD b2546 0.63 +0.9
2,674,532 - yphD b2546 0.68 +1.5
2,674,836 - +0.6
2,674,884 - +0.2
2,674,904 + +0.2
2,674,904 + +0.1
2,675,002 + -0.3
2,675,130 - yphE b2547 0.17 +0.1
2,675,252 - yphE b2547 0.25 +1.5
2,675,436 - yphE b2547 0.37 +1.2
2,675,657 + yphE b2547 0.52 +0.9
2,675,657 + yphE b2547 0.52 -1.3
2,675,706 - yphE b2547 0.55 -1.0

Or see this region's nucleotide sequence