Experiment: Cholin acetate 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfcM and yfcA overlap by 1 nucleotides yfcA and mepA overlap by 1 nucleotides mepA and aroC are separated by 3 nucleotides
b2326: yfcM - hypothetical protein (NCBI), at 2,442,225 to 2,442,773
yfcM
b2327: yfcA - conserved inner membrane protein (NCBI), at 2,442,773 to 2,443,582
yfcA
b2328: mepA - penicillin-insensitive murein endopeptidase (NCBI), at 2,443,582 to 2,444,406
mepA
b2329: aroC - chorismate synthase (NCBI), at 2,444,410 to 2,445,495
aroC
Position (kb)
2443
2444
2445 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2442.583 kb on - strand, within yfcM at 2442.647 kb on - strand, within yfcM at 2442.647 kb on - strand, within yfcM at 2442.696 kb on - strand, within yfcM at 2442.758 kb on + strand at 2442.758 kb on + strand at 2442.758 kb on + strand at 2442.766 kb on - strand at 2442.766 kb on - strand at 2442.766 kb on - strand at 2442.766 kb on - strand at 2442.777 kb on - strand at 2442.807 kb on - strand at 2442.887 kb on + strand, within yfcA at 2442.887 kb on + strand, within yfcA at 2442.887 kb on + strand, within yfcA at 2442.887 kb on + strand, within yfcA at 2442.898 kb on + strand, within yfcA at 2442.906 kb on - strand, within yfcA at 2442.906 kb on - strand, within yfcA at 2442.948 kb on - strand, within yfcA at 2442.976 kb on + strand, within yfcA at 2442.985 kb on + strand, within yfcA at 2443.071 kb on - strand, within yfcA at 2443.161 kb on + strand, within yfcA at 2443.293 kb on - strand, within yfcA at 2443.330 kb on + strand, within yfcA at 2443.700 kb on - strand, within mepA at 2443.700 kb on - strand, within mepA at 2443.705 kb on + strand, within mepA at 2443.770 kb on + strand, within mepA at 2443.778 kb on - strand, within mepA at 2443.778 kb on - strand, within mepA at 2443.782 kb on - strand, within mepA at 2443.797 kb on - strand, within mepA at 2443.797 kb on - strand, within mepA at 2443.817 kb on - strand, within mepA at 2443.817 kb on - strand, within mepA at 2443.897 kb on + strand, within mepA at 2444.082 kb on - strand, within mepA at 2444.245 kb on - strand, within mepA at 2444.245 kb on - strand, within mepA at 2444.256 kb on + strand, within mepA at 2444.552 kb on - strand, within aroC at 2444.844 kb on + strand, within aroC at 2444.866 kb on - strand, within aroC at 2444.923 kb on - strand, within aroC at 2444.997 kb on - strand, within aroC at 2444.997 kb on - strand, within aroC at 2445.075 kb on + strand, within aroC at 2445.075 kb on + strand, within aroC at 2445.180 kb on + strand, within aroC at 2445.213 kb on + strand, within aroC at 2445.213 kb on + strand, within aroC at 2445.219 kb on - strand, within aroC at 2445.219 kb on - strand, within aroC at 2445.244 kb on + strand, within aroC at 2445.326 kb on + strand, within aroC at 2445.326 kb on + strand, within aroC at 2445.337 kb on - strand, within aroC at 2445.337 kb on - strand, within aroC
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 75 mM remove 2,442,583 - yfcM b2326 0.65 +0.3 2,442,647 - yfcM b2326 0.77 -0.0 2,442,647 - yfcM b2326 0.77 -0.7 2,442,696 - yfcM b2326 0.86 +1.3 2,442,758 + +0.5 2,442,758 + -2.0 2,442,758 + -0.2 2,442,766 - -0.2 2,442,766 - +1.8 2,442,766 - -0.6 2,442,766 - +0.8 2,442,777 - +0.4 2,442,807 - +0.3 2,442,887 + yfcA b2327 0.14 +0.4 2,442,887 + yfcA b2327 0.14 +0.3 2,442,887 + yfcA b2327 0.14 -0.2 2,442,887 + yfcA b2327 0.14 -0.0 2,442,898 + yfcA b2327 0.15 -0.4 2,442,906 - yfcA b2327 0.16 -0.2 2,442,906 - yfcA b2327 0.16 -0.2 2,442,948 - yfcA b2327 0.22 +0.3 2,442,976 + yfcA b2327 0.25 -0.7 2,442,985 + yfcA b2327 0.26 -2.0 2,443,071 - yfcA b2327 0.37 +1.0 2,443,161 + yfcA b2327 0.48 -0.3 2,443,293 - yfcA b2327 0.64 -0.6 2,443,330 + yfcA b2327 0.69 +0.0 2,443,700 - mepA b2328 0.14 +0.1 2,443,700 - mepA b2328 0.14 +0.5 2,443,705 + mepA b2328 0.15 +0.6 2,443,770 + mepA b2328 0.23 +0.1 2,443,778 - mepA b2328 0.24 -0.5 2,443,778 - mepA b2328 0.24 +0.8 2,443,782 - mepA b2328 0.24 -1.0 2,443,797 - mepA b2328 0.26 +1.4 2,443,797 - mepA b2328 0.26 -0.9 2,443,817 - mepA b2328 0.28 -0.6 2,443,817 - mepA b2328 0.28 -0.7 2,443,897 + mepA b2328 0.38 -1.2 2,444,082 - mepA b2328 0.61 -0.2 2,444,245 - mepA b2328 0.80 -0.2 2,444,245 - mepA b2328 0.80 -0.4 2,444,256 + mepA b2328 0.82 +0.1 2,444,552 - aroC b2329 0.13 -0.2 2,444,844 + aroC b2329 0.40 +0.8 2,444,866 - aroC b2329 0.42 -0.3 2,444,923 - aroC b2329 0.47 -0.7 2,444,997 - aroC b2329 0.54 -0.2 2,444,997 - aroC b2329 0.54 -0.2 2,445,075 + aroC b2329 0.61 +0.4 2,445,075 + aroC b2329 0.61 +3.4 2,445,180 + aroC b2329 0.71 +0.0 2,445,213 + aroC b2329 0.74 +0.3 2,445,213 + aroC b2329 0.74 +1.1 2,445,219 - aroC b2329 0.74 +1.6 2,445,219 - aroC b2329 0.74 +0.4 2,445,244 + aroC b2329 0.77 +0.4 2,445,326 + aroC b2329 0.84 +0.5 2,445,326 + aroC b2329 0.84 +0.7 2,445,337 - aroC b2329 0.85 +0.3 2,445,337 - aroC b2329 0.85 -0.5
Or see this region's nucleotide sequence