Experiment: Cholin acetate 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt acrA and acrR are separated by 141 nucleotides acrR and kefA are separated by 127 nucleotides kefA and ybaM are separated by 211 nucleotides ybaM and priC are separated by 13 nucleotides priC and ybaN are separated by 69 nucleotides
b0463: acrA - multidrug efflux system (NCBI), at 483,650 to 484,843
acrA
b0464: acrR - DNA-binding transcriptional repressor (NCBI), at 484,985 to 485,632
acrR
b0465: kefA - fused conserved protein (NCBI), at 485,760 to 489,122
kefA
b0466: ybaM - hypothetical protein (NCBI), at 489,334 to 489,495
ybaM
b0467: priC - primosomal replication protein N'' (NCBI), at 489,509 to 490,036
priC
b0468: ybaN - conserved inner membrane protein (NCBI), at 490,106 to 490,483
ybaN
Position (kb) 485
486
487
488
489
490 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 484.917 kb on - strand at 484.917 kb on - strand at 485.062 kb on + strand, within acrR at 485.071 kb on + strand, within acrR at 485.071 kb on + strand, within acrR at 485.145 kb on - strand, within acrR at 485.175 kb on - strand, within acrR at 485.193 kb on - strand, within acrR at 485.193 kb on - strand, within acrR at 485.199 kb on + strand, within acrR at 485.199 kb on + strand, within acrR at 485.239 kb on + strand, within acrR at 485.239 kb on + strand, within acrR at 485.246 kb on + strand, within acrR at 485.246 kb on + strand, within acrR at 485.255 kb on - strand, within acrR at 485.255 kb on - strand, within acrR at 485.273 kb on + strand, within acrR at 485.281 kb on + strand, within acrR at 485.283 kb on + strand, within acrR at 485.284 kb on + strand, within acrR at 485.285 kb on - strand, within acrR at 485.285 kb on - strand, within acrR at 485.289 kb on + strand, within acrR at 485.289 kb on + strand, within acrR at 485.289 kb on - strand, within acrR at 485.289 kb on - strand, within acrR at 485.299 kb on - strand, within acrR at 485.358 kb on + strand, within acrR at 485.358 kb on + strand, within acrR at 485.358 kb on + strand, within acrR at 485.358 kb on + strand, within acrR at 485.360 kb on + strand, within acrR at 485.360 kb on + strand, within acrR at 485.363 kb on + strand, within acrR at 485.364 kb on + strand, within acrR at 485.365 kb on - strand, within acrR at 485.366 kb on - strand, within acrR at 485.368 kb on - strand, within acrR at 485.374 kb on - strand, within acrR at 485.374 kb on - strand, within acrR at 485.380 kb on - strand, within acrR at 485.388 kb on - strand, within acrR at 485.394 kb on + strand, within acrR at 485.402 kb on - strand, within acrR at 485.403 kb on + strand, within acrR at 485.403 kb on + strand, within acrR at 485.408 kb on + strand, within acrR at 485.411 kb on - strand, within acrR at 485.411 kb on - strand, within acrR at 485.411 kb on - strand, within acrR at 485.416 kb on - strand, within acrR at 485.480 kb on + strand, within acrR at 485.503 kb on - strand, within acrR at 485.527 kb on + strand, within acrR at 485.574 kb on + strand at 485.575 kb on + strand at 485.575 kb on + strand at 485.582 kb on - strand at 485.582 kb on - strand at 485.584 kb on + strand at 485.586 kb on + strand at 485.586 kb on + strand at 485.586 kb on + strand at 485.586 kb on + strand at 485.586 kb on + strand at 485.586 kb on + strand at 485.586 kb on + strand at 485.592 kb on - strand at 485.594 kb on - strand at 485.596 kb on + strand at 485.601 kb on - strand at 485.604 kb on - strand at 485.606 kb on - strand at 485.608 kb on + strand at 485.608 kb on + strand at 485.616 kb on - strand at 485.616 kb on - strand at 485.665 kb on + strand at 485.763 kb on + strand at 485.763 kb on + strand at 485.776 kb on + strand at 485.782 kb on - strand at 485.837 kb on + strand at 485.869 kb on - strand at 485.873 kb on - strand at 485.898 kb on - strand at 485.977 kb on + strand at 485.977 kb on + strand at 486.032 kb on - strand at 486.092 kb on + strand at 486.143 kb on + strand, within kefA at 486.226 kb on - strand, within kefA at 486.226 kb on - strand, within kefA at 486.251 kb on + strand, within kefA at 486.335 kb on - strand, within kefA at 486.350 kb on - strand, within kefA at 486.350 kb on - strand, within kefA at 486.363 kb on - strand, within kefA at 486.363 kb on - strand, within kefA at 486.513 kb on - strand, within kefA at 486.652 kb on - strand, within kefA at 486.652 kb on - strand, within kefA at 486.654 kb on - strand, within kefA at 486.654 kb on - strand, within kefA at 486.805 kb on + strand, within kefA at 486.915 kb on - strand, within kefA at 486.917 kb on + strand, within kefA at 486.925 kb on - strand, within kefA at 486.925 kb on - strand, within kefA at 486.925 kb on - strand, within kefA at 486.925 kb on - strand, within kefA at 486.926 kb on - strand, within kefA at 486.937 kb on + strand, within kefA at 486.948 kb on + strand, within kefA at 487.092 kb on - strand, within kefA at 487.092 kb on - strand, within kefA at 487.172 kb on + strand, within kefA at 487.173 kb on + strand, within kefA at 487.193 kb on - strand, within kefA at 487.306 kb on + strand, within kefA at 487.317 kb on + strand, within kefA at 487.506 kb on + strand, within kefA at 487.549 kb on - strand, within kefA at 487.549 kb on - strand, within kefA at 487.562 kb on + strand, within kefA at 487.570 kb on - strand, within kefA at 487.588 kb on - strand, within kefA at 487.620 kb on - strand, within kefA at 487.620 kb on - strand, within kefA at 487.625 kb on + strand, within kefA at 487.642 kb on + strand, within kefA at 487.776 kb on + strand, within kefA at 487.776 kb on + strand, within kefA at 487.820 kb on + strand, within kefA at 487.820 kb on + strand, within kefA at 487.820 kb on + strand, within kefA at 487.836 kb on - strand at 487.836 kb on - strand, within kefA at 487.886 kb on + strand, within kefA at 487.889 kb on - strand, within kefA at 487.890 kb on + strand, within kefA at 487.890 kb on + strand, within kefA at 487.898 kb on - strand, within kefA at 487.898 kb on - strand, within kefA at 487.901 kb on + strand, within kefA at 487.911 kb on + strand, within kefA at 487.911 kb on + strand, within kefA at 487.912 kb on - strand, within kefA at 487.912 kb on - strand, within kefA at 487.992 kb on + strand, within kefA at 488.012 kb on + strand, within kefA at 488.063 kb on - strand, within kefA at 488.078 kb on - strand, within kefA at 488.167 kb on + strand, within kefA at 488.283 kb on + strand, within kefA at 488.319 kb on + strand, within kefA at 488.356 kb on + strand, within kefA at 488.366 kb on + strand, within kefA at 488.374 kb on + strand, within kefA at 488.397 kb on + strand, within kefA at 488.397 kb on + strand, within kefA at 488.411 kb on - strand, within kefA at 488.421 kb on + strand, within kefA at 488.580 kb on + strand, within kefA at 488.627 kb on + strand, within kefA at 488.753 kb on + strand, within kefA at 488.753 kb on + strand, within kefA at 488.758 kb on - strand, within kefA at 488.853 kb on - strand at 488.923 kb on + strand at 488.931 kb on - strand at 488.931 kb on - strand at 488.951 kb on + strand at 488.961 kb on + strand at 488.970 kb on - strand at 488.970 kb on - strand at 488.970 kb on - strand at 488.975 kb on - strand at 488.975 kb on - strand at 488.982 kb on + strand at 488.982 kb on + strand at 488.998 kb on - strand at 489.042 kb on - strand at 489.065 kb on + strand at 489.065 kb on + strand at 489.258 kb on - strand at 489.258 kb on - strand at 489.258 kb on - strand at 489.258 kb on - strand at 489.499 kb on - strand at 489.499 kb on - strand at 489.512 kb on - strand at 489.512 kb on - strand at 489.571 kb on + strand, within priC at 489.585 kb on - strand, within priC at 489.670 kb on - strand, within priC at 489.677 kb on - strand, within priC at 489.705 kb on - strand, within priC at 489.768 kb on + strand, within priC at 489.847 kb on - strand, within priC at 489.847 kb on - strand, within priC at 489.901 kb on - strand, within priC at 489.901 kb on - strand, within priC at 489.904 kb on - strand, within priC at 489.942 kb on + strand, within priC at 489.959 kb on + strand, within priC at 489.967 kb on - strand, within priC
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 75 mM remove 484,917 - +0.2 484,917 - +1.2 485,062 + acrR b0464 0.12 -1.0 485,071 + acrR b0464 0.13 +1.0 485,071 + acrR b0464 0.13 +0.6 485,145 - acrR b0464 0.25 -0.6 485,175 - acrR b0464 0.29 -0.4 485,193 - acrR b0464 0.32 +0.4 485,193 - acrR b0464 0.32 +2.0 485,199 + acrR b0464 0.33 -0.4 485,199 + acrR b0464 0.33 -0.0 485,239 + acrR b0464 0.39 +0.2 485,239 + acrR b0464 0.39 +1.0 485,246 + acrR b0464 0.40 -0.3 485,246 + acrR b0464 0.40 -0.6 485,255 - acrR b0464 0.42 -0.4 485,255 - acrR b0464 0.42 -0.8 485,273 + acrR b0464 0.44 -0.0 485,281 + acrR b0464 0.46 -0.0 485,283 + acrR b0464 0.46 +0.1 485,284 + acrR b0464 0.46 -0.7 485,285 - acrR b0464 0.46 +0.4 485,285 - acrR b0464 0.46 -0.0 485,289 + acrR b0464 0.47 +0.4 485,289 + acrR b0464 0.47 +0.2 485,289 - acrR b0464 0.47 +0.3 485,289 - acrR b0464 0.47 -1.5 485,299 - acrR b0464 0.48 +0.4 485,358 + acrR b0464 0.58 -0.0 485,358 + acrR b0464 0.58 -0.0 485,358 + acrR b0464 0.58 +0.1 485,358 + acrR b0464 0.58 -0.5 485,360 + acrR b0464 0.58 -0.6 485,360 + acrR b0464 0.58 -0.6 485,363 + acrR b0464 0.58 +1.0 485,364 + acrR b0464 0.58 -0.1 485,365 - acrR b0464 0.59 +1.4 485,366 - acrR b0464 0.59 +0.1 485,368 - acrR b0464 0.59 -1.0 485,374 - acrR b0464 0.60 -0.6 485,374 - acrR b0464 0.60 -0.6 485,380 - acrR b0464 0.61 -0.6 485,388 - acrR b0464 0.62 +0.8 485,394 + acrR b0464 0.63 +0.3 485,402 - acrR b0464 0.64 +0.1 485,403 + acrR b0464 0.65 -0.8 485,403 + acrR b0464 0.65 +0.4 485,408 + acrR b0464 0.65 -1.2 485,411 - acrR b0464 0.66 +0.8 485,411 - acrR b0464 0.66 -0.0 485,411 - acrR b0464 0.66 -0.2 485,416 - acrR b0464 0.67 +1.0 485,480 + acrR b0464 0.76 -0.7 485,503 - acrR b0464 0.80 -0.3 485,527 + acrR b0464 0.84 -0.6 485,574 + -0.0 485,575 + +1.0 485,575 + -0.6 485,582 - +0.2 485,582 - +1.4 485,584 + -0.1 485,586 + -0.0 485,586 + -1.6 485,586 + +0.8 485,586 + +0.4 485,586 + -0.3 485,586 + -0.3 485,586 + -0.1 485,592 - +0.2 485,594 - +0.3 485,596 + -0.2 485,601 - +0.1 485,604 - +0.9 485,606 - +1.1 485,608 + +2.0 485,608 + -0.7 485,616 - +0.8 485,616 - +1.0 485,665 + +0.7 485,763 + +0.4 485,763 + +0.7 485,776 + +0.1 485,782 - -0.2 485,837 + +0.1 485,869 - -0.0 485,873 - +0.1 485,898 - +0.4 485,977 + +0.3 485,977 + +0.5 486,032 - +0.3 486,092 + -0.1 486,143 + kefA b0465 0.11 +0.7 486,226 - kefA b0465 0.14 +1.0 486,226 - kefA b0465 0.14 +0.2 486,251 + kefA b0465 0.15 +0.2 486,335 - kefA b0465 0.17 +0.1 486,350 - kefA b0465 0.18 +1.0 486,350 - kefA b0465 0.18 +0.7 486,363 - kefA b0465 0.18 -1.2 486,363 - kefA b0465 0.18 -0.2 486,513 - kefA b0465 0.22 -0.7 486,652 - kefA b0465 0.27 +1.0 486,652 - kefA b0465 0.27 -0.5 486,654 - kefA b0465 0.27 -0.6 486,654 - kefA b0465 0.27 -0.0 486,805 + kefA b0465 0.31 -0.0 486,915 - kefA b0465 0.34 -0.3 486,917 + kefA b0465 0.34 -0.5 486,925 - kefA b0465 0.35 +0.6 486,925 - kefA b0465 0.35 -1.6 486,925 - kefA b0465 0.35 +1.1 486,925 - kefA b0465 0.35 +0.6 486,926 - kefA b0465 0.35 +0.3 486,937 + kefA b0465 0.35 +1.0 486,948 + kefA b0465 0.35 +1.4 487,092 - kefA b0465 0.40 +2.0 487,092 - kefA b0465 0.40 +0.2 487,172 + kefA b0465 0.42 +0.6 487,173 + kefA b0465 0.42 -0.0 487,193 - kefA b0465 0.43 +0.5 487,306 + kefA b0465 0.46 -0.8 487,317 + kefA b0465 0.46 +1.6 487,506 + kefA b0465 0.52 -0.8 487,549 - kefA b0465 0.53 +1.4 487,549 - kefA b0465 0.53 +1.0 487,562 + kefA b0465 0.54 -0.1 487,570 - kefA b0465 0.54 -0.9 487,588 - kefA b0465 0.54 +0.5 487,620 - kefA b0465 0.55 +0.3 487,620 - kefA b0465 0.55 +0.2 487,625 + kefA b0465 0.55 -1.6 487,642 + kefA b0465 0.56 -0.4 487,776 + kefA b0465 0.60 -1.6 487,776 + kefA b0465 0.60 +2.0 487,820 + kefA b0465 0.61 +2.0 487,820 + kefA b0465 0.61 +0.4 487,820 + kefA b0465 0.61 -0.0 487,836 - -0.0 487,836 - kefA b0465 0.62 -1.0 487,886 + kefA b0465 0.63 -0.0 487,889 - kefA b0465 0.63 +0.2 487,890 + kefA b0465 0.63 -0.8 487,890 + kefA b0465 0.63 -0.6 487,898 - kefA b0465 0.64 +0.6 487,898 - kefA b0465 0.64 +1.0 487,901 + kefA b0465 0.64 -0.5 487,911 + kefA b0465 0.64 -0.0 487,911 + kefA b0465 0.64 +3.0 487,912 - kefA b0465 0.64 -0.4 487,912 - kefA b0465 0.64 +0.7 487,992 + kefA b0465 0.66 +0.1 488,012 + kefA b0465 0.67 +0.8 488,063 - kefA b0465 0.68 +0.6 488,078 - kefA b0465 0.69 +0.5 488,167 + kefA b0465 0.72 -0.2 488,283 + kefA b0465 0.75 +0.2 488,319 + kefA b0465 0.76 -0.0 488,356 + kefA b0465 0.77 -0.2 488,366 + kefA b0465 0.77 -0.6 488,374 + kefA b0465 0.78 +0.4 488,397 + kefA b0465 0.78 -0.1 488,397 + kefA b0465 0.78 +0.4 488,411 - kefA b0465 0.79 -0.4 488,421 + kefA b0465 0.79 -0.1 488,580 + kefA b0465 0.84 -0.8 488,627 + kefA b0465 0.85 -0.6 488,753 + kefA b0465 0.89 +1.0 488,753 + kefA b0465 0.89 +0.4 488,758 - kefA b0465 0.89 -0.6 488,853 - +0.5 488,923 + +0.3 488,931 - +0.6 488,931 - -0.6 488,951 + +0.2 488,961 + -0.6 488,970 - +0.2 488,970 - +0.1 488,970 - -0.1 488,975 - -0.8 488,975 - +2.8 488,982 + +0.1 488,982 + -0.2 488,998 - +1.0 489,042 - +0.4 489,065 + +0.2 489,065 + -2.7 489,258 - +0.2 489,258 - -0.7 489,258 - -0.0 489,258 - +0.1 489,499 - -0.0 489,499 - +2.3 489,512 - -0.6 489,512 - -0.3 489,571 + priC b0467 0.12 +0.1 489,585 - priC b0467 0.14 -0.1 489,670 - priC b0467 0.30 +0.2 489,677 - priC b0467 0.32 +0.1 489,705 - priC b0467 0.37 +0.2 489,768 + priC b0467 0.49 +0.6 489,847 - priC b0467 0.64 +0.6 489,847 - priC b0467 0.64 -0.6 489,901 - priC b0467 0.74 -0.6 489,901 - priC b0467 0.74 +0.5 489,904 - priC b0467 0.75 -0.6 489,942 + priC b0467 0.82 -0.4 489,959 + priC b0467 0.85 +0.8 489,967 - priC b0467 0.87 -0.8
Or see this region's nucleotide sequence