Experiment: L-Valine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_04110 and PS417_04115 are separated by 141 nucleotides PS417_04115 and PS417_04120 are separated by 24 nucleotides PS417_04120 and PS417_04125 are separated by 79 nucleotides
PS417_04110: PS417_04110 - cytochrome D ubiquinol oxidase subunit III, at 924,709 to 925,146
_04110
PS417_04115: PS417_04115 - alpha/beta hydrolase, at 925,288 to 925,923
_04115
PS417_04120: PS417_04120 - tryptophan--tRNA ligase, at 925,948 to 927,303
_04120
PS417_04125: PS417_04125 - ATPase, at 927,383 to 928,477
_04125
Position (kb) 925
926
927
928 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 925.203 kb on - strand at 925.203 kb on - strand at 925.245 kb on - strand at 925.426 kb on - strand, within PS417_04115 at 925.426 kb on - strand, within PS417_04115 at 925.433 kb on + strand, within PS417_04115 at 925.474 kb on - strand, within PS417_04115 at 925.524 kb on + strand, within PS417_04115 at 925.801 kb on + strand, within PS417_04115 at 925.935 kb on + strand at 927.479 kb on + strand at 927.550 kb on + strand, within PS417_04125 at 927.890 kb on + strand, within PS417_04125
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (C) remove 925,203 - -1.8 925,203 - -2.8 925,245 - +0.3 925,426 - PS417_04115 0.22 -1.3 925,426 - PS417_04115 0.22 +1.0 925,433 + PS417_04115 0.23 -1.1 925,474 - PS417_04115 0.29 -2.2 925,524 + PS417_04115 0.37 -1.8 925,801 + PS417_04115 0.81 +0.6 925,935 + +1.5 927,479 + +0.1 927,550 + PS417_04125 0.15 -1.2 927,890 + PS417_04125 0.46 -2.0
Or see this region's nucleotide sequence