Strain Fitness in Bosea sp. OAE506 around ABIE41_RS09525

Experiment: R2A outgrowth control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABIE41_RS09515 and ABIE41_RS09520 are separated by 12 nucleotidesABIE41_RS09520 and ABIE41_RS09525 are separated by 28 nucleotidesABIE41_RS09525 and ABIE41_RS09530 are separated by 86 nucleotides ABIE41_RS09515: ABIE41_RS09515 - protein TolQ, at 1,965,593 to 1,966,303 _RS09515 ABIE41_RS09520: ABIE41_RS09520 - biopolymer transporter ExbD, at 1,966,316 to 1,966,765 _RS09520 ABIE41_RS09525: ABIE41_RS09525 - cell envelope integrity protein TolA, at 1,966,794 to 1,967,990 _RS09525 ABIE41_RS09530: ABIE41_RS09530 - Tol-Pal system beta propeller repeat protein TolB, at 1,968,077 to 1,969,333 _RS09530 Position (kb) 1966 1967 1968Strain fitness (log2 ratio) -2 -1 0 1 2at 1967.983 kb on + strandat 1967.983 kb on + strandat 1967.983 kb on + strandat 1967.983 kb on + strandat 1967.983 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A outgrowth control
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1,967,983 + -0.2
1,967,983 + +0.1
1,967,983 + +2.7
1,967,983 + -2.3
1,967,983 + +0.3

Or see this region's nucleotide sequence