Experiment: L-Valine (C) 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFR28_04885 and PFR28_04886 are separated by 88 nucleotides PFR28_04886 and PFR28_04887 overlap by 4 nucleotides PFR28_04887 and PFR28_04888 are separated by 130 nucleotides PFR28_04888 and PFR28_04889 are separated by 36 nucleotides
PFR28_04885: PFR28_04885 - GMP/IMP nucleotidase YrfG, at 5,579,487 to 5,580,149
_04885
PFR28_04886: PFR28_04886 - ADP compounds hydrolase NudE, at 5,580,238 to 5,580,804
_04886
PFR28_04887: PFR28_04887 - '3'(2'),5'-bisphosphate nucleotidase CysQ' transl_table=11, at 5,580,801 to 5,581,628
_04887
PFR28_04888: PFR28_04888 - hypothetical protein, at 5,581,759 to 5,582,214
_04888
PFR28_04889: PFR28_04889 - C4-dicarboxylate transport transcriptional regulatory protein DctD, at 5,582,251 to 5,583,642
_04889
Position (kb) 5580
5581
5582 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 5579.807 kb on + strand, within PFR28_04885 at 5579.808 kb on - strand, within PFR28_04885 at 5579.950 kb on + strand, within PFR28_04885 at 5579.950 kb on + strand, within PFR28_04885 at 5579.951 kb on - strand, within PFR28_04885 at 5579.951 kb on - strand, within PFR28_04885 at 5580.076 kb on + strand, within PFR28_04885 at 5580.076 kb on + strand, within PFR28_04885 at 5580.076 kb on + strand, within PFR28_04885 at 5580.077 kb on - strand, within PFR28_04885 at 5580.077 kb on - strand, within PFR28_04885 at 5580.077 kb on - strand, within PFR28_04885 at 5580.077 kb on - strand, within PFR28_04885 at 5580.119 kb on + strand at 5580.149 kb on + strand at 5580.149 kb on + strand at 5580.150 kb on - strand at 5580.196 kb on + strand at 5580.196 kb on + strand at 5580.196 kb on + strand at 5580.196 kb on + strand at 5580.196 kb on + strand at 5580.196 kb on + strand at 5580.197 kb on - strand at 5580.197 kb on - strand at 5580.205 kb on + strand at 5580.205 kb on + strand at 5580.205 kb on + strand at 5580.206 kb on - strand at 5580.206 kb on - strand at 5580.207 kb on + strand at 5580.207 kb on + strand at 5580.207 kb on + strand at 5580.207 kb on + strand at 5580.207 kb on + strand at 5580.207 kb on + strand at 5580.207 kb on + strand at 5580.207 kb on + strand at 5580.207 kb on + strand at 5580.207 kb on + strand at 5580.207 kb on + strand at 5580.207 kb on + strand at 5580.207 kb on + strand at 5580.207 kb on + strand at 5580.207 kb on + strand at 5580.207 kb on + strand at 5580.207 kb on + strand at 5580.208 kb on - strand at 5580.208 kb on - strand at 5580.208 kb on - strand at 5580.208 kb on - strand at 5580.208 kb on - strand at 5580.208 kb on - strand at 5580.208 kb on - strand at 5580.208 kb on - strand at 5580.208 kb on - strand at 5580.208 kb on - strand at 5580.208 kb on - strand at 5580.208 kb on - strand at 5580.210 kb on + strand at 5580.210 kb on + strand at 5580.210 kb on + strand at 5580.211 kb on - strand at 5580.211 kb on - strand at 5580.211 kb on - strand at 5580.237 kb on + strand at 5580.257 kb on + strand at 5580.257 kb on + strand at 5580.257 kb on + strand at 5580.257 kb on + strand at 5580.258 kb on - strand at 5580.343 kb on + strand, within PFR28_04886 at 5580.343 kb on + strand, within PFR28_04886 at 5580.343 kb on + strand, within PFR28_04886 at 5580.344 kb on - strand, within PFR28_04886 at 5580.344 kb on - strand, within PFR28_04886 at 5580.344 kb on - strand, within PFR28_04886 at 5580.344 kb on - strand, within PFR28_04886 at 5580.344 kb on - strand, within PFR28_04886 at 5580.344 kb on - strand, within PFR28_04886 at 5580.433 kb on + strand, within PFR28_04886 at 5580.433 kb on + strand, within PFR28_04886 at 5580.433 kb on + strand, within PFR28_04886 at 5580.433 kb on + strand, within PFR28_04886 at 5580.433 kb on + strand, within PFR28_04886 at 5580.433 kb on + strand, within PFR28_04886 at 5580.433 kb on + strand, within PFR28_04886 at 5580.434 kb on - strand, within PFR28_04886 at 5580.434 kb on - strand, within PFR28_04886 at 5580.434 kb on - strand, within PFR28_04886 at 5580.434 kb on - strand, within PFR28_04886 at 5580.434 kb on - strand, within PFR28_04886 at 5580.434 kb on - strand, within PFR28_04886 at 5580.454 kb on + strand, within PFR28_04886 at 5580.454 kb on + strand, within PFR28_04886 at 5580.592 kb on + strand, within PFR28_04886 at 5580.593 kb on - strand, within PFR28_04886 at 5580.757 kb on + strand at 5580.757 kb on + strand at 5580.757 kb on + strand at 5580.758 kb on - strand at 5580.758 kb on - strand at 5580.758 kb on - strand at 5580.807 kb on + strand at 5580.807 kb on + strand at 5580.808 kb on - strand at 5580.808 kb on - strand at 5580.808 kb on - strand at 5580.808 kb on - strand at 5581.185 kb on + strand, within PFR28_04887 at 5581.185 kb on + strand, within PFR28_04887 at 5581.186 kb on - strand, within PFR28_04887 at 5581.283 kb on + strand, within PFR28_04887 at 5581.307 kb on + strand, within PFR28_04887 at 5581.428 kb on + strand, within PFR28_04887 at 5581.429 kb on - strand, within PFR28_04887 at 5581.719 kb on + strand at 5581.775 kb on + strand at 5581.775 kb on + strand at 5581.775 kb on + strand at 5581.775 kb on + strand at 5581.775 kb on + strand at 5581.775 kb on + strand at 5581.775 kb on + strand at 5581.775 kb on + strand at 5581.775 kb on + strand at 5581.775 kb on + strand at 5581.775 kb on + strand at 5581.776 kb on - strand at 5581.865 kb on + strand, within PFR28_04888 at 5581.866 kb on - strand, within PFR28_04888 at 5581.866 kb on - strand, within PFR28_04888 at 5581.946 kb on + strand, within PFR28_04888 at 5581.947 kb on - strand, within PFR28_04888 at 5581.947 kb on - strand, within PFR28_04888 at 5581.974 kb on + strand, within PFR28_04888 at 5581.974 kb on + strand, within PFR28_04888 at 5581.974 kb on + strand, within PFR28_04888 at 5581.974 kb on + strand, within PFR28_04888 at 5581.974 kb on + strand, within PFR28_04888 at 5581.975 kb on - strand, within PFR28_04888 at 5582.049 kb on - strand, within PFR28_04888 at 5582.195 kb on + strand at 5582.247 kb on + strand at 5582.248 kb on - strand at 5582.248 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (C) 10 mM remove 5,579,807 + PFR28_04885 0.48 -0.7 5,579,808 - PFR28_04885 0.48 +0.1 5,579,950 + PFR28_04885 0.70 -1.7 5,579,950 + PFR28_04885 0.70 +0.7 5,579,951 - PFR28_04885 0.70 +0.3 5,579,951 - PFR28_04885 0.70 +1.2 5,580,076 + PFR28_04885 0.89 -0.8 5,580,076 + PFR28_04885 0.89 -0.8 5,580,076 + PFR28_04885 0.89 +0.6 5,580,077 - PFR28_04885 0.89 +1.7 5,580,077 - PFR28_04885 0.89 -0.1 5,580,077 - PFR28_04885 0.89 -0.4 5,580,077 - PFR28_04885 0.89 -1.1 5,580,119 + +1.2 5,580,149 + -1.5 5,580,149 + -1.3 5,580,150 - +0.1 5,580,196 + +0.7 5,580,196 + -1.9 5,580,196 + +0.6 5,580,196 + +1.1 5,580,196 + +0.2 5,580,196 + -0.3 5,580,197 - +0.5 5,580,197 - +0.3 5,580,205 + +0.1 5,580,205 + -0.6 5,580,205 + +0.9 5,580,206 - -0.4 5,580,206 - -0.1 5,580,207 + -0.4 5,580,207 + +0.8 5,580,207 + +1.0 5,580,207 + +0.6 5,580,207 + -0.4 5,580,207 + -1.8 5,580,207 + +0.1 5,580,207 + -1.1 5,580,207 + +0.2 5,580,207 + +0.1 5,580,207 + -0.7 5,580,207 + +0.2 5,580,207 + +0.0 5,580,207 + +0.1 5,580,207 + -0.5 5,580,207 + -0.0 5,580,207 + -0.5 5,580,208 - -1.7 5,580,208 - -1.5 5,580,208 - +3.0 5,580,208 - -0.2 5,580,208 - -0.6 5,580,208 - +0.1 5,580,208 - -0.1 5,580,208 - -0.1 5,580,208 - +0.1 5,580,208 - -0.3 5,580,208 - +1.2 5,580,208 - +0.1 5,580,210 + -1.1 5,580,210 + -1.7 5,580,210 + -1.0 5,580,211 - +0.0 5,580,211 - +0.0 5,580,211 - -0.6 5,580,237 + -0.6 5,580,257 + +0.1 5,580,257 + -0.5 5,580,257 + -0.1 5,580,257 + +1.0 5,580,258 - -1.1 5,580,343 + PFR28_04886 0.19 -0.0 5,580,343 + PFR28_04886 0.19 -0.3 5,580,343 + PFR28_04886 0.19 +0.1 5,580,344 - PFR28_04886 0.19 +0.3 5,580,344 - PFR28_04886 0.19 +1.6 5,580,344 - PFR28_04886 0.19 -3.0 5,580,344 - PFR28_04886 0.19 -1.4 5,580,344 - PFR28_04886 0.19 -2.8 5,580,344 - PFR28_04886 0.19 -1.6 5,580,433 + PFR28_04886 0.34 +0.5 5,580,433 + PFR28_04886 0.34 +1.0 5,580,433 + PFR28_04886 0.34 +0.2 5,580,433 + PFR28_04886 0.34 +0.0 5,580,433 + PFR28_04886 0.34 +0.2 5,580,433 + PFR28_04886 0.34 +0.4 5,580,433 + PFR28_04886 0.34 +0.0 5,580,434 - PFR28_04886 0.35 -1.6 5,580,434 - PFR28_04886 0.35 -0.6 5,580,434 - PFR28_04886 0.35 -1.5 5,580,434 - PFR28_04886 0.35 -0.8 5,580,434 - PFR28_04886 0.35 -0.7 5,580,434 - PFR28_04886 0.35 -0.2 5,580,454 + PFR28_04886 0.38 +0.6 5,580,454 + PFR28_04886 0.38 +0.7 5,580,592 + PFR28_04886 0.62 +0.5 5,580,593 - PFR28_04886 0.63 -2.1 5,580,757 + +0.2 5,580,757 + -0.7 5,580,757 + +1.0 5,580,758 - -0.9 5,580,758 - -1.0 5,580,758 - -1.0 5,580,807 + -0.8 5,580,807 + +0.6 5,580,808 - -3.5 5,580,808 - -2.9 5,580,808 - -4.1 5,580,808 - -2.0 5,581,185 + PFR28_04887 0.46 -2.6 5,581,185 + PFR28_04887 0.46 -3.6 5,581,186 - PFR28_04887 0.46 -2.4 5,581,283 + PFR28_04887 0.58 -4.6 5,581,307 + PFR28_04887 0.61 -1.5 5,581,428 + PFR28_04887 0.76 -1.0 5,581,429 - PFR28_04887 0.76 -2.5 5,581,719 + +0.3 5,581,775 + +0.1 5,581,775 + +0.8 5,581,775 + +0.8 5,581,775 + +1.1 5,581,775 + +0.4 5,581,775 + +1.5 5,581,775 + +0.4 5,581,775 + -0.4 5,581,775 + -2.4 5,581,775 + +0.9 5,581,775 + +0.9 5,581,776 - +0.5 5,581,865 + PFR28_04888 0.23 -0.7 5,581,866 - PFR28_04888 0.23 +0.6 5,581,866 - PFR28_04888 0.23 +0.8 5,581,946 + PFR28_04888 0.41 +0.6 5,581,947 - PFR28_04888 0.41 +0.0 5,581,947 - PFR28_04888 0.41 +2.9 5,581,974 + PFR28_04888 0.47 -0.2 5,581,974 + PFR28_04888 0.47 +0.6 5,581,974 + PFR28_04888 0.47 +1.2 5,581,974 + PFR28_04888 0.47 +1.0 5,581,974 + PFR28_04888 0.47 +0.5 5,581,975 - PFR28_04888 0.47 +0.3 5,582,049 - PFR28_04888 0.64 +1.6 5,582,195 + +0.6 5,582,247 + -0.1 5,582,248 - -2.0 5,582,248 - -0.1
Or see this region's nucleotide sequence