Strain Fitness in Escherichia coli BL21 around ECD_04302

Experiment: lambda1857_phage_12.5_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00982 and ECD_04302 are separated by 12 nucleotidesECD_04302 and ECD_00983 are separated by 79 nucleotides ECD_00982: ECD_00982 - cytochrome bd-II oxidase, subunit II, at 1,044,385 to 1,045,521 _00982 ECD_04302: ECD_04302 - putative cytochrome bd-II oxidase subunit, at 1,045,534 to 1,045,626 _04302 ECD_00983: ECD_00983 - phosphoanhydride phosphorylase, at 1,045,706 to 1,047,004 _00983 Position (kb) 1045 1046Strain fitness (log2 ratio) -2 -1 0 1 2at 1044.620 kb on - strand, within ECD_00982at 1044.643 kb on + strand, within ECD_00982at 1044.947 kb on + strand, within ECD_00982at 1045.399 kb on - strand, within ECD_00982at 1045.399 kb on - strand, within ECD_00982at 1045.441 kb on + strandat 1045.541 kb on - strandat 1045.544 kb on + strand, within ECD_04302at 1045.633 kb on + strandat 1045.684 kb on + strandat 1045.932 kb on + strand, within ECD_00983at 1045.933 kb on - strand, within ECD_00983at 1046.026 kb on + strand, within ECD_00983at 1046.107 kb on - strand, within ECD_00983at 1046.122 kb on + strand, within ECD_00983at 1046.146 kb on + strand, within ECD_00983at 1046.147 kb on - strand, within ECD_00983at 1046.308 kb on + strand, within ECD_00983

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Per-strain Table

Position Strand Gene LocusTag Fraction lambda1857_phage_12.5_MOI
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1,044,620 - ECD_00982 0.21 +0.4
1,044,643 + ECD_00982 0.23 +1.2
1,044,947 + ECD_00982 0.49 -0.2
1,045,399 - ECD_00982 0.89 +0.1
1,045,399 - ECD_00982 0.89 -1.8
1,045,441 + +0.8
1,045,541 - -0.8
1,045,544 + ECD_04302 0.11 -1.1
1,045,633 + +1.4
1,045,684 + -1.1
1,045,932 + ECD_00983 0.17 +1.2
1,045,933 - ECD_00983 0.17 -1.2
1,046,026 + ECD_00983 0.25 -2.7
1,046,107 - ECD_00983 0.31 +0.1
1,046,122 + ECD_00983 0.32 -0.6
1,046,146 + ECD_00983 0.34 +2.0
1,046,147 - ECD_00983 0.34 -1.0
1,046,308 + ECD_00983 0.46 -0.2

Or see this region's nucleotide sequence