Strain Fitness in Escherichia coli BL21 around ECD_04134

Experiment: lambda1857_phage_12.5_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_04133 and ECD_04134 are separated by 216 nucleotidesECD_04134 and ECD_04135 are separated by 3 nucleotides ECD_04133: ECD_04133 - L-idonate 5-dehydrogenase, NAD-binding, at 4,404,968 to 4,405,999 _04133 ECD_04134: ECD_04134 - D-gluconate kinase, thermosensitive, at 4,406,216 to 4,406,779 _04134 ECD_04135: ECD_04135 - broad specificity NADPH-dependent aldehyde reductase, Zn-containing, at 4,406,783 to 4,407,802 _04135 Position (kb) 4406 4407Strain fitness (log2 ratio) -3 -2 -1 0 1at 4405.249 kb on + strand, within ECD_04133at 4405.344 kb on - strand, within ECD_04133at 4405.478 kb on - strand, within ECD_04133at 4405.478 kb on - strand, within ECD_04133at 4405.482 kb on - strand, within ECD_04133at 4405.496 kb on + strand, within ECD_04133at 4405.612 kb on + strand, within ECD_04133at 4405.787 kb on - strand, within ECD_04133at 4405.932 kb on - strandat 4406.039 kb on - strandat 4406.048 kb on + strandat 4406.049 kb on - strandat 4406.049 kb on - strandat 4406.096 kb on - strandat 4406.213 kb on + strandat 4406.529 kb on + strand, within ECD_04134at 4406.540 kb on + strand, within ECD_04134at 4406.601 kb on + strand, within ECD_04134at 4406.736 kb on + strandat 4406.736 kb on + strandat 4406.821 kb on + strandat 4406.920 kb on + strand, within ECD_04135at 4406.920 kb on + strand, within ECD_04135at 4406.921 kb on - strand, within ECD_04135at 4406.984 kb on - strand, within ECD_04135at 4406.984 kb on - strand, within ECD_04135at 4407.042 kb on + strand, within ECD_04135at 4407.202 kb on + strand, within ECD_04135at 4407.208 kb on + strand, within ECD_04135at 4407.249 kb on - strand, within ECD_04135at 4407.249 kb on - strand, within ECD_04135at 4407.249 kb on - strand, within ECD_04135at 4407.307 kb on + strand, within ECD_04135at 4407.520 kb on + strand, within ECD_04135at 4407.748 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction lambda1857_phage_12.5_MOI
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4,405,249 + ECD_04133 0.27 -1.6
4,405,344 - ECD_04133 0.36 +1.2
4,405,478 - ECD_04133 0.49 +1.1
4,405,478 - ECD_04133 0.49 -0.7
4,405,482 - ECD_04133 0.50 -1.4
4,405,496 + ECD_04133 0.51 +0.3
4,405,612 + ECD_04133 0.62 -0.9
4,405,787 - ECD_04133 0.79 +0.5
4,405,932 - -1.8
4,406,039 - -0.5
4,406,048 + -2.7
4,406,049 - +1.1
4,406,049 - +0.6
4,406,096 - -0.6
4,406,213 + -1.3
4,406,529 + ECD_04134 0.55 +0.8
4,406,540 + ECD_04134 0.57 +0.7
4,406,601 + ECD_04134 0.68 +0.9
4,406,736 + +1.3
4,406,736 + -1.2
4,406,821 + -0.6
4,406,920 + ECD_04135 0.13 -1.5
4,406,920 + ECD_04135 0.13 +0.6
4,406,921 - ECD_04135 0.14 -1.4
4,406,984 - ECD_04135 0.20 +0.5
4,406,984 - ECD_04135 0.20 +1.4
4,407,042 + ECD_04135 0.25 -0.6
4,407,202 + ECD_04135 0.41 +0.8
4,407,208 + ECD_04135 0.42 +1.7
4,407,249 - ECD_04135 0.46 +1.7
4,407,249 - ECD_04135 0.46 -1.5
4,407,249 - ECD_04135 0.46 +0.7
4,407,307 + ECD_04135 0.51 -3.2
4,407,520 + ECD_04135 0.72 -1.5
4,407,748 - +0.6

Or see this region's nucleotide sequence