Strain Fitness in Escherichia coli BL21 around ECD_03196

Experiment: lambda1857_phage_12.5_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_03193 and ECD_03194 are separated by 125 nucleotidesECD_03194 and ECD_03195 are separated by 7 nucleotidesECD_03195 and ECD_03196 overlap by 1 nucleotidesECD_03196 and ECD_03197 overlap by 1 nucleotidesECD_03197 and ECD_03198 are separated by 166 nucleotides ECD_03193: ECD_03193 - 30S ribosomal subunit protein S12, at 3,333,779 to 3,334,153 _03193 ECD_03194: ECD_03194 - mnm(5)-s(2)U34-tRNA synthesis 2-thiolation protein, at 3,334,279 to 3,334,566 _03194 ECD_03195: ECD_03195 - mnm(5)-s(2)U34-tRNA synthesis 2-thiolation protein, at 3,334,574 to 3,334,933 _03195 ECD_03196: ECD_03196 - sulfurtransferase for 2-thiolation step of mnm(5)-s(2)U34-tRNA synthesis, at 3,334,933 to 3,335,319 _03196 ECD_03197: ECD_03197 - putative PAS domain-containing DNA-binding transcriptional regulator, at 3,335,319 to 3,336,041 _03197 ECD_03198: ECD_03198 - FKBP-type peptidyl-prolyl cis-trans isomerase (rotamase), at 3,336,208 to 3,337,020 _03198 Position (kb) 3334 3335 3336Strain fitness (log2 ratio) -2 -1 0 1 2at 3334.261 kb on + strandat 3334.264 kb on - strandat 3334.264 kb on - strandat 3334.264 kb on - strandat 3335.366 kb on - strandat 3335.372 kb on - strandat 3335.372 kb on - strandat 3335.372 kb on - strandat 3335.372 kb on - strandat 3335.427 kb on - strand, within ECD_03197at 3335.427 kb on - strand, within ECD_03197at 3335.671 kb on - strand, within ECD_03197at 3335.671 kb on - strand, within ECD_03197at 3335.671 kb on - strand, within ECD_03197at 3335.716 kb on - strand, within ECD_03197at 3335.719 kb on - strand, within ECD_03197at 3335.739 kb on - strand, within ECD_03197at 3335.751 kb on - strand, within ECD_03197at 3335.790 kb on - strand, within ECD_03197at 3335.790 kb on - strand, within ECD_03197at 3335.790 kb on - strand, within ECD_03197at 3335.794 kb on - strand, within ECD_03197at 3335.808 kb on - strand, within ECD_03197at 3335.808 kb on - strand, within ECD_03197at 3335.808 kb on - strand, within ECD_03197at 3335.835 kb on - strand, within ECD_03197at 3335.835 kb on - strand, within ECD_03197at 3335.835 kb on - strand, within ECD_03197at 3335.835 kb on - strand, within ECD_03197at 3336.152 kb on + strandat 3336.152 kb on + strandat 3336.202 kb on + strandat 3336.292 kb on + strand, within ECD_03198

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Per-strain Table

Position Strand Gene LocusTag Fraction lambda1857_phage_12.5_MOI
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3,334,261 + -0.1
3,334,264 - -0.7
3,334,264 - +0.1
3,334,264 - -1.9
3,335,366 - +1.5
3,335,372 - +0.9
3,335,372 - +0.5
3,335,372 - +0.6
3,335,372 - +0.9
3,335,427 - ECD_03197 0.15 +1.6
3,335,427 - ECD_03197 0.15 +0.8
3,335,671 - ECD_03197 0.49 +1.1
3,335,671 - ECD_03197 0.49 +0.6
3,335,671 - ECD_03197 0.49 +1.9
3,335,716 - ECD_03197 0.55 +1.2
3,335,719 - ECD_03197 0.55 -0.0
3,335,739 - ECD_03197 0.58 +1.6
3,335,751 - ECD_03197 0.60 -2.2
3,335,790 - ECD_03197 0.65 +0.8
3,335,790 - ECD_03197 0.65 +2.3
3,335,790 - ECD_03197 0.65 -2.2
3,335,794 - ECD_03197 0.66 +1.3
3,335,808 - ECD_03197 0.68 -2.6
3,335,808 - ECD_03197 0.68 -1.5
3,335,808 - ECD_03197 0.68 -0.3
3,335,835 - ECD_03197 0.71 +2.5
3,335,835 - ECD_03197 0.71 +0.9
3,335,835 - ECD_03197 0.71 +1.5
3,335,835 - ECD_03197 0.71 +0.1
3,336,152 + +1.2
3,336,152 + +0.5
3,336,202 + +1.7
3,336,292 + ECD_03198 0.10 -0.1

Or see this region's nucleotide sequence