Strain Fitness in Escherichia coli BL21 around ECD_02648

Experiment: lambda1857_phage_12.5_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_02646 and ECD_02647 overlap by 82 nucleotidesECD_02647 and ECD_02648 are separated by 627 nucleotidesECD_02648 and ECD_02649 are separated by 118 nucleotides ECD_02646: ECD_02646 - IS1 protein InsB, at 2,765,863 to 2,766,366 _02646 ECD_02647: ECD_02647 - IS1 protein InsA, at 2,766,285 to 2,766,560 _02647 ECD_02648: ECD_02648 - L-fuculose phosphate aldolase, at 2,767,188 to 2,768,132 _02648 ECD_02649: ECD_02649 - L-ribulokinase AraB-like protein, at 2,768,251 to 2,769,879 _02649 Position (kb) 2767 2768 2769Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2766.674 kb on - strandat 2766.690 kb on - strandat 2766.690 kb on - strandat 2766.718 kb on + strandat 2766.718 kb on + strandat 2766.719 kb on - strandat 2766.719 kb on - strandat 2766.897 kb on - strandat 2766.897 kb on - strandat 2768.042 kb on - strandat 2768.069 kb on + strandat 2768.190 kb on + strandat 2768.191 kb on - strandat 2768.202 kb on - strandat 2768.202 kb on - strandat 2768.229 kb on - strandat 2768.229 kb on - strandat 2768.313 kb on + strandat 2768.513 kb on - strand, within ECD_02649at 2768.560 kb on - strand, within ECD_02649at 2768.589 kb on + strand, within ECD_02649at 2768.589 kb on + strand, within ECD_02649at 2768.589 kb on + strand, within ECD_02649at 2768.589 kb on + strand, within ECD_02649at 2768.589 kb on + strand, within ECD_02649at 2768.590 kb on - strand, within ECD_02649at 2768.669 kb on - strand, within ECD_02649at 2768.743 kb on + strand, within ECD_02649at 2768.974 kb on + strand, within ECD_02649at 2769.109 kb on - strand, within ECD_02649

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Per-strain Table

Position Strand Gene LocusTag Fraction lambda1857_phage_12.5_MOI
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2,766,674 - -1.3
2,766,690 - +0.9
2,766,690 - -1.5
2,766,718 + -0.5
2,766,718 + +2.1
2,766,719 - -0.6
2,766,719 - +1.8
2,766,897 - -1.3
2,766,897 - -1.2
2,768,042 - +1.2
2,768,069 + -0.0
2,768,190 + -0.3
2,768,191 - -0.9
2,768,202 - -2.0
2,768,202 - -0.2
2,768,229 - -2.9
2,768,229 - -0.2
2,768,313 + +0.2
2,768,513 - ECD_02649 0.16 +0.1
2,768,560 - ECD_02649 0.19 -1.0
2,768,589 + ECD_02649 0.21 -1.6
2,768,589 + ECD_02649 0.21 +0.1
2,768,589 + ECD_02649 0.21 +0.4
2,768,589 + ECD_02649 0.21 +0.3
2,768,589 + ECD_02649 0.21 -0.6
2,768,590 - ECD_02649 0.21 +0.7
2,768,669 - ECD_02649 0.26 +1.2
2,768,743 + ECD_02649 0.30 +1.0
2,768,974 + ECD_02649 0.44 -1.5
2,769,109 - ECD_02649 0.53 -0.1

Or see this region's nucleotide sequence