Strain Fitness in Escherichia coli BL21 around ECD_02328

Experiment: lambda1857_phage_12.5_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_02327 and ECD_02328 are separated by 163 nucleotidesECD_02328 and ECD_02329 are separated by 3 nucleotides ECD_02327: ECD_02327 - repressor for murPQ, MurNAc 6-P inducible, at 2,417,155 to 2,418,012 _02327 ECD_02328: ECD_02328 - N-acetylmuramic acid 6-phosphate (MurNAc-6-P) etherase, at 2,418,176 to 2,419,072 _02328 ECD_02329: ECD_02329 - N-acetylmuramic acid permease, EIIBC component, PTS system, at 2,419,076 to 2,420,500 _02329 Position (kb) 2418 2419 2420Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2417.217 kb on + strandat 2417.218 kb on - strandat 2417.218 kb on - strandat 2417.290 kb on - strand, within ECD_02327at 2417.449 kb on - strand, within ECD_02327at 2417.449 kb on - strand, within ECD_02327at 2417.498 kb on - strand, within ECD_02327at 2417.498 kb on - strand, within ECD_02327at 2417.793 kb on - strand, within ECD_02327at 2417.793 kb on - strand, within ECD_02327at 2417.801 kb on - strand, within ECD_02327at 2418.006 kb on - strandat 2418.006 kb on - strandat 2418.058 kb on - strandat 2418.058 kb on - strandat 2418.159 kb on + strandat 2418.159 kb on + strandat 2418.160 kb on - strandat 2418.162 kb on + strandat 2418.163 kb on - strandat 2418.277 kb on + strand, within ECD_02328at 2418.374 kb on + strand, within ECD_02328at 2418.726 kb on + strand, within ECD_02328at 2418.895 kb on + strand, within ECD_02328at 2418.945 kb on + strand, within ECD_02328at 2419.117 kb on + strandat 2419.163 kb on - strandat 2419.163 kb on - strandat 2419.185 kb on + strandat 2419.185 kb on + strandat 2419.186 kb on - strandat 2419.495 kb on - strand, within ECD_02329at 2419.558 kb on + strand, within ECD_02329at 2419.611 kb on - strand, within ECD_02329at 2419.684 kb on + strand, within ECD_02329at 2419.759 kb on + strand, within ECD_02329at 2419.759 kb on + strand, within ECD_02329at 2419.760 kb on - strand, within ECD_02329at 2419.889 kb on + strand, within ECD_02329at 2419.900 kb on + strand, within ECD_02329at 2419.901 kb on - strand, within ECD_02329at 2419.930 kb on + strand, within ECD_02329at 2419.930 kb on + strand, within ECD_02329at 2419.930 kb on + strand, within ECD_02329at 2419.930 kb on + strand, within ECD_02329at 2419.930 kb on + strand, within ECD_02329at 2419.931 kb on - strand, within ECD_02329at 2419.931 kb on - strand, within ECD_02329at 2419.946 kb on - strand, within ECD_02329at 2419.961 kb on - strand, within ECD_02329

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Per-strain Table

Position Strand Gene LocusTag Fraction lambda1857_phage_12.5_MOI
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2,417,217 + -2.0
2,417,218 - +1.5
2,417,218 - -0.5
2,417,290 - ECD_02327 0.16 +2.1
2,417,449 - ECD_02327 0.34 +0.9
2,417,449 - ECD_02327 0.34 +1.1
2,417,498 - ECD_02327 0.40 +2.1
2,417,498 - ECD_02327 0.40 +1.1
2,417,793 - ECD_02327 0.74 +0.7
2,417,793 - ECD_02327 0.74 +0.0
2,417,801 - ECD_02327 0.75 +2.0
2,418,006 - +0.3
2,418,006 - -0.6
2,418,058 - +0.8
2,418,058 - +0.9
2,418,159 + +0.1
2,418,159 + -1.0
2,418,160 - -0.7
2,418,162 + -0.2
2,418,163 - +0.7
2,418,277 + ECD_02328 0.11 +0.8
2,418,374 + ECD_02328 0.22 +0.3
2,418,726 + ECD_02328 0.61 +1.5
2,418,895 + ECD_02328 0.80 +1.0
2,418,945 + ECD_02328 0.86 +0.5
2,419,117 + +0.6
2,419,163 - +1.2
2,419,163 - -2.2
2,419,185 + +2.0
2,419,185 + +1.1
2,419,186 - -0.6
2,419,495 - ECD_02329 0.29 -3.2
2,419,558 + ECD_02329 0.34 +0.7
2,419,611 - ECD_02329 0.38 -0.0
2,419,684 + ECD_02329 0.43 -1.9
2,419,759 + ECD_02329 0.48 +0.3
2,419,759 + ECD_02329 0.48 -2.6
2,419,760 - ECD_02329 0.48 -0.4
2,419,889 + ECD_02329 0.57 +0.3
2,419,900 + ECD_02329 0.58 -0.5
2,419,901 - ECD_02329 0.58 -0.1
2,419,930 + ECD_02329 0.60 -0.2
2,419,930 + ECD_02329 0.60 +1.5
2,419,930 + ECD_02329 0.60 -0.2
2,419,930 + ECD_02329 0.60 +2.4
2,419,930 + ECD_02329 0.60 -3.6
2,419,931 - ECD_02329 0.60 +1.3
2,419,931 - ECD_02329 0.60 -0.2
2,419,946 - ECD_02329 0.61 -1.1
2,419,961 - ECD_02329 0.62 +0.5

Or see this region's nucleotide sequence