Experiment: lambda1857_phage_12.5_MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECD_02188 and ECD_02189 overlap by 1 nucleotides ECD_02189 and ECD_02190 are separated by 14 nucleotides ECD_02190 and ECD_02191 overlap by 4 nucleotides
ECD_02188: ECD_02188 - O-succinylbenzoyl-CoA synthase, at 2,265,174 to 2,266,136
_02188
ECD_02189: ECD_02189 - dihydroxynaphthoic acid synthetase, at 2,266,136 to 2,266,993
_02189
ECD_02190: ECD_02190 - 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase, at 2,267,008 to 2,267,766
_02190
ECD_02191: ECD_02191 - 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase; SEPHCHC synthase, at 2,267,763 to 2,269,433
_02191
Position (kb)
2267
2268 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2266.046 kb on - strand at 2266.046 kb on - strand at 2266.486 kb on - strand, within ECD_02189 at 2266.549 kb on + strand, within ECD_02189 at 2266.609 kb on - strand, within ECD_02189 at 2266.686 kb on - strand, within ECD_02189 at 2266.686 kb on - strand, within ECD_02189 at 2267.195 kb on - strand, within ECD_02190 at 2267.559 kb on + strand, within ECD_02190 at 2267.598 kb on + strand, within ECD_02190 at 2267.636 kb on + strand, within ECD_02190 at 2267.637 kb on - strand, within ECD_02190 at 2267.637 kb on - strand, within ECD_02190 at 2267.637 kb on - strand, within ECD_02190 at 2267.637 kb on - strand, within ECD_02190 at 2267.767 kb on - strand at 2268.095 kb on - strand, within ECD_02191 at 2268.206 kb on - strand, within ECD_02191 at 2268.267 kb on - strand, within ECD_02191 at 2268.446 kb on + strand, within ECD_02191
Per-strain Table
Position Strand Gene LocusTag Fraction lambda1857_phage_12.5_MOI remove 2,266,046 - +1.0 2,266,046 - -2.3 2,266,486 - ECD_02189 0.41 -1.6 2,266,549 + ECD_02189 0.48 -0.9 2,266,609 - ECD_02189 0.55 -2.8 2,266,686 - ECD_02189 0.64 -1.1 2,266,686 - ECD_02189 0.64 +1.8 2,267,195 - ECD_02190 0.25 -3.3 2,267,559 + ECD_02190 0.73 -0.6 2,267,598 + ECD_02190 0.78 -2.1 2,267,636 + ECD_02190 0.83 -3.3 2,267,637 - ECD_02190 0.83 -1.2 2,267,637 - ECD_02190 0.83 +0.8 2,267,637 - ECD_02190 0.83 +0.6 2,267,637 - ECD_02190 0.83 +1.5 2,267,767 - -2.1 2,268,095 - ECD_02191 0.20 -0.2 2,268,206 - ECD_02191 0.27 +1.4 2,268,267 - ECD_02191 0.30 +0.6 2,268,446 + ECD_02191 0.41 +0.0
Or see this region's nucleotide sequence