Strain Fitness in Escherichia coli BL21 around ECD_02189

Experiment: lambda1857_phage_12.5_MOI

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntECD_02187 and ECD_02188 overlap by 4 nucleotidesECD_02188 and ECD_02189 overlap by 1 nucleotidesECD_02189 and ECD_02190 are separated by 14 nucleotidesECD_02190 and ECD_02191 overlap by 4 nucleotides ECD_02187: ECD_02187 - O-succinylbenzoate-CoA ligase, at 2,263,822 to 2,265,177 _02187 ECD_02188: ECD_02188 - O-succinylbenzoyl-CoA synthase, at 2,265,174 to 2,266,136 _02188 ECD_02189: ECD_02189 - dihydroxynaphthoic acid synthetase, at 2,266,136 to 2,266,993 _02189 ECD_02190: ECD_02190 - 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase, at 2,267,008 to 2,267,766 _02190 ECD_02191: ECD_02191 - 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase; SEPHCHC synthase, at 2,267,763 to 2,269,433 _02191 Position (kb) 2266 2267Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2265.330 kb on + strand, within ECD_02188at 2265.442 kb on - strand, within ECD_02188at 2265.608 kb on - strand, within ECD_02188at 2265.728 kb on - strand, within ECD_02188at 2265.768 kb on - strand, within ECD_02188at 2265.848 kb on + strand, within ECD_02188at 2265.849 kb on - strand, within ECD_02188at 2265.849 kb on - strand, within ECD_02188at 2265.932 kb on + strand, within ECD_02188at 2265.933 kb on - strand, within ECD_02188at 2266.046 kb on - strandat 2266.046 kb on - strandat 2266.486 kb on - strand, within ECD_02189at 2266.549 kb on + strand, within ECD_02189at 2266.609 kb on - strand, within ECD_02189at 2266.686 kb on - strand, within ECD_02189at 2266.686 kb on - strand, within ECD_02189at 2267.195 kb on - strand, within ECD_02190at 2267.559 kb on + strand, within ECD_02190at 2267.598 kb on + strand, within ECD_02190at 2267.636 kb on + strand, within ECD_02190at 2267.637 kb on - strand, within ECD_02190at 2267.637 kb on - strand, within ECD_02190at 2267.637 kb on - strand, within ECD_02190at 2267.637 kb on - strand, within ECD_02190at 2267.767 kb on - strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction lambda1857_phage_12.5_MOI
remove
2,265,330 + ECD_02188 0.16 +0.2
2,265,442 - ECD_02188 0.28 -1.6
2,265,608 - ECD_02188 0.45 +3.1
2,265,728 - ECD_02188 0.58 +1.6
2,265,768 - ECD_02188 0.62 +1.3
2,265,848 + ECD_02188 0.70 -0.1
2,265,849 - ECD_02188 0.70 -1.6
2,265,849 - ECD_02188 0.70 +0.2
2,265,932 + ECD_02188 0.79 +0.0
2,265,933 - ECD_02188 0.79 -0.8
2,266,046 - +1.0
2,266,046 - -2.3
2,266,486 - ECD_02189 0.41 -1.6
2,266,549 + ECD_02189 0.48 -0.9
2,266,609 - ECD_02189 0.55 -2.8
2,266,686 - ECD_02189 0.64 -1.1
2,266,686 - ECD_02189 0.64 +1.8
2,267,195 - ECD_02190 0.25 -3.3
2,267,559 + ECD_02190 0.73 -0.6
2,267,598 + ECD_02190 0.78 -2.1
2,267,636 + ECD_02190 0.83 -3.3
2,267,637 - ECD_02190 0.83 -1.2
2,267,637 - ECD_02190 0.83 +0.8
2,267,637 - ECD_02190 0.83 +0.6
2,267,637 - ECD_02190 0.83 +1.5
2,267,767 - -2.1

Or see this region's nucleotide sequence