Strain Fitness in Escherichia coli BL21 around ECD_01842

Experiment: lambda1857_phage_12.5_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01840 and ECD_01841 are separated by 40 nucleotidesECD_01841 and ECD_01842 overlap by 4 nucleotidesECD_01842 and ECD_01843 are separated by 164 nucleotides ECD_01840: ECD_01840 - MAPEG family inner membrane protein, at 1,898,184 to 1,898,579 _01840 ECD_01841: ECD_01841 - carboxy-SAM synthase, at 1,898,620 to 1,899,363 _01841 ECD_01842: ECD_01842 - tRNA (cmo5U34)-carboxymethyltransferase, carboxy-SAM-dependent, at 1,899,360 to 1,900,331 _01842 ECD_01843: ECD_01843 - trimethylamine N-oxide reductase system III, catalytic subunit, at 1,900,496 to 1,902,925 _01843 Position (kb) 1899 1900 1901Strain fitness (log2 ratio) -3 -2 -1 0 1at 1898.546 kb on + strandat 1898.587 kb on + strandat 1898.587 kb on + strandat 1898.734 kb on + strand, within ECD_01841at 1898.760 kb on + strand, within ECD_01841at 1898.761 kb on - strand, within ECD_01841at 1898.922 kb on + strand, within ECD_01841at 1898.951 kb on + strand, within ECD_01841at 1899.444 kb on - strandat 1899.522 kb on + strand, within ECD_01842at 1899.587 kb on + strand, within ECD_01842at 1899.648 kb on + strand, within ECD_01842at 1900.450 kb on + strandat 1900.552 kb on - strandat 1900.880 kb on - strand, within ECD_01843at 1900.988 kb on - strand, within ECD_01843

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Per-strain Table

Position Strand Gene LocusTag Fraction lambda1857_phage_12.5_MOI
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1,898,546 + -1.4
1,898,587 + -1.6
1,898,587 + +0.2
1,898,734 + ECD_01841 0.15 -2.2
1,898,760 + ECD_01841 0.19 -1.7
1,898,761 - ECD_01841 0.19 +0.5
1,898,922 + ECD_01841 0.41 -1.0
1,898,951 + ECD_01841 0.44 +0.6
1,899,444 - -0.5
1,899,522 + ECD_01842 0.17 -2.4
1,899,587 + ECD_01842 0.23 +0.5
1,899,648 + ECD_01842 0.30 -3.1
1,900,450 + -1.3
1,900,552 - +0.4
1,900,880 - ECD_01843 0.16 +1.1
1,900,988 - ECD_01843 0.20 -1.0

Or see this region's nucleotide sequence