Experiment: lambda1857_phage_12.5_MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECD_01645 and ECD_01646 are separated by 310 nucleotides ECD_01646 and ECD_01647 are separated by 63 nucleotides ECD_01647 and ECD_01648 are separated by 148 nucleotides ECD_01648 and ECD_01649 are separated by 12 nucleotides
ECD_01645: ECD_01645 - pyruvate kinase I, at 1,700,967 to 1,702,379
_01645
ECD_01646: ECD_01646 - murein lipoprotein, at 1,702,690 to 1,702,926
_01646
ECD_01647: ECD_01647 - murein L,D-transpeptidase, at 1,702,990 to 1,703,994
_01647
ECD_01648: ECD_01648 - sulfur acceptor protein, at 1,704,143 to 1,704,559
_01648
ECD_01649: ECD_01649 - cysteine desulfurase, stimulated by SufE; selenocysteine lyase, PLP-dependent, at 1,704,572 to 1,705,792
_01649
Position (kb) 1702
1703
1704 Strain fitness (log2 ratio) -1
0
1 at 1702.109 kb on + strand, within ECD_01645 at 1702.130 kb on + strand, within ECD_01645 at 1702.312 kb on + strand at 1702.654 kb on + strand at 1702.675 kb on + strand at 1702.924 kb on + strand at 1703.626 kb on - strand, within ECD_01647 at 1704.144 kb on + strand at 1704.640 kb on - strand at 1704.679 kb on - strand at 1704.682 kb on - strand at 1704.915 kb on + strand, within ECD_01649
Per-strain Table
Position Strand Gene LocusTag Fraction lambda1857_phage_12.5_MOI remove 1,702,109 + ECD_01645 0.81 +0.0 1,702,130 + ECD_01645 0.82 -1.0 1,702,312 + +1.5 1,702,654 + -0.4 1,702,675 + +1.7 1,702,924 + +1.0 1,703,626 - ECD_01647 0.63 -0.4 1,704,144 + +0.4 1,704,640 - +1.6 1,704,679 - -0.9 1,704,682 - +0.8 1,704,915 + ECD_01649 0.28 -1.7
Or see this region's nucleotide sequence