Strain Fitness in Escherichia coli BL21 around ECD_01316

Experiment: lambda1857_phage_12.5_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01315 and ECD_01316 are separated by 175 nucleotidesECD_01316 and ECD_01317 are separated by 329 nucleotidesECD_01317 and ECD_01318 are separated by 20 nucleotides ECD_01315: ECD_01315 - p-aminobenzoyl-glutamate hydrolase, A subunit, at 1,388,423 to 1,389,733 _01315 ECD_01316: ECD_01316 - putative DNA-binding transcriptional regulator of abgABT operon, at 1,389,909 to 1,390,817 _01316 ECD_01317: ECD_01317 - DNA endonuclease, at 1,391,147 to 1,391,710 _01317 ECD_01318: ECD_01318 - diguanylate cyclase, csgD regulator, at 1,391,731 to 1,392,963 _01318 Position (kb) 1389 1390 1391Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1389.222 kb on - strand, within ECD_01315at 1389.522 kb on - strand, within ECD_01315at 1389.567 kb on - strand, within ECD_01315at 1389.799 kb on - strandat 1389.828 kb on + strandat 1389.886 kb on - strandat 1389.998 kb on + strandat 1391.063 kb on + strandat 1391.084 kb on + strandat 1391.116 kb on + strandat 1391.127 kb on + strandat 1391.555 kb on + strand, within ECD_01317at 1391.555 kb on + strand, within ECD_01317at 1391.555 kb on + strand, within ECD_01317at 1391.555 kb on + strand, within ECD_01317at 1391.649 kb on + strand, within ECD_01317at 1391.653 kb on + strand, within ECD_01317

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Per-strain Table

Position Strand Gene LocusTag Fraction lambda1857_phage_12.5_MOI
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1,389,222 - ECD_01315 0.61 +3.2
1,389,522 - ECD_01315 0.84 -0.3
1,389,567 - ECD_01315 0.87 +2.2
1,389,799 - -0.6
1,389,828 + -0.1
1,389,886 - -0.8
1,389,998 + -0.0
1,391,063 + +2.6
1,391,084 + -1.3
1,391,116 + +1.0
1,391,127 + +1.5
1,391,555 + ECD_01317 0.72 +1.7
1,391,555 + ECD_01317 0.72 -1.5
1,391,555 + ECD_01317 0.72 -1.6
1,391,555 + ECD_01317 0.72 +1.3
1,391,649 + ECD_01317 0.89 +2.4
1,391,653 + ECD_01317 0.90 -2.4

Or see this region's nucleotide sequence