Strain Fitness in Escherichia coli BL21 around ECD_01173

Experiment: lambda1857_phage_12.5_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01172 and ECD_01173 are separated by 319 nucleotidesECD_01173 and ECD_01174 are separated by 10 nucleotidesECD_01174 and ECD_01175 are separated by 10 nucleotides ECD_01172: ECD_01172 - periplasmic trehalase, at 1,233,827 to 1,235,524 _01172 ECD_01173: ECD_01173 - putative dihydroxyacetone-specific PTS enzymes: HPr, EI components, at 1,235,844 to 1,237,262 _01173 ECD_01174: ECD_01174 - dihydroxyacetone kinase, C-terminal domain, at 1,237,273 to 1,237,905 _01174 ECD_01175: ECD_01175 - dihydroxyacetone kinase, PTS-dependent, dihydroxyacetone-binding subunit, at 1,237,916 to 1,238,986 _01175 Position (kb) 1235 1236 1237 1238Strain fitness (log2 ratio) -1 0 1 2 3at 1235.055 kb on + strand, within ECD_01172at 1235.088 kb on + strand, within ECD_01172at 1235.470 kb on + strandat 1236.006 kb on - strand, within ECD_01173at 1236.013 kb on - strand, within ECD_01173at 1236.072 kb on - strand, within ECD_01173at 1236.084 kb on - strand, within ECD_01173at 1236.156 kb on - strand, within ECD_01173at 1236.298 kb on - strand, within ECD_01173at 1236.306 kb on - strand, within ECD_01173at 1236.624 kb on - strand, within ECD_01173at 1236.731 kb on - strand, within ECD_01173at 1237.236 kb on - strandat 1237.246 kb on - strandat 1237.309 kb on - strandat 1237.309 kb on - strandat 1237.396 kb on - strand, within ECD_01174at 1237.402 kb on - strand, within ECD_01174at 1237.402 kb on - strand, within ECD_01174at 1237.783 kb on - strand, within ECD_01174at 1238.015 kb on - strandat 1238.048 kb on - strand, within ECD_01175

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Per-strain Table

Position Strand Gene LocusTag Fraction lambda1857_phage_12.5_MOI
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1,235,055 + ECD_01172 0.72 +1.3
1,235,088 + ECD_01172 0.74 +0.5
1,235,470 + +0.9
1,236,006 - ECD_01173 0.11 -0.0
1,236,013 - ECD_01173 0.12 +2.9
1,236,072 - ECD_01173 0.16 +3.5
1,236,084 - ECD_01173 0.17 +3.0
1,236,156 - ECD_01173 0.22 +2.3
1,236,298 - ECD_01173 0.32 +2.0
1,236,306 - ECD_01173 0.33 +1.5
1,236,624 - ECD_01173 0.55 +0.9
1,236,731 - ECD_01173 0.63 +1.7
1,237,236 - +1.0
1,237,246 - +1.2
1,237,309 - +1.5
1,237,309 - -1.0
1,237,396 - ECD_01174 0.19 +1.8
1,237,402 - ECD_01174 0.20 -0.3
1,237,402 - ECD_01174 0.20 +1.3
1,237,783 - ECD_01174 0.81 -1.7
1,238,015 - -0.4
1,238,048 - ECD_01175 0.12 +1.3

Or see this region's nucleotide sequence