Strain Fitness in Escherichia coli BL21 around ECD_01133

Experiment: lambda1857_phage_12.5_MOI

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntECD_01131 and ECD_01132 are separated by 53 nucleotidesECD_01132 and ECD_01133 are separated by 9 nucleotidesECD_01133 and ECD_01134 are separated by 171 nucleotides ECD_01131: ECD_01131 - tRNA(Gln,Lys,Glu) U34 2-thiouridylase, at 1,196,034 to 1,197,140 _01131 ECD_01132: ECD_01132 - bifunctional thiamine pyrimidine pyrophosphate hydrolase and thiamine pyrophosphate hydrolase, at 1,197,194 to 1,197,655 _01132 ECD_01133: ECD_01133 - 23S rRNA pseudouridine(2457) synthase, at 1,197,665 to 1,198,318 _01133 ECD_01134: ECD_01134 - isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase, at 1,198,490 to 1,199,740 _01134 Position (kb) 1197 1198 1199Strain fitness (log2 ratio) -2 -1 0 1 2at 1197.212 kb on - strandat 1197.224 kb on - strandat 1197.581 kb on - strand, within ECD_01132at 1197.715 kb on - strandat 1197.801 kb on + strand, within ECD_01133at 1197.802 kb on - strand, within ECD_01133at 1197.802 kb on - strand, within ECD_01133at 1197.802 kb on - strand, within ECD_01133at 1197.804 kb on - strand, within ECD_01133at 1197.804 kb on - strand, within ECD_01133at 1197.804 kb on - strand, within ECD_01133at 1197.804 kb on - strand, within ECD_01133at 1197.804 kb on - strand, within ECD_01133at 1197.804 kb on - strand, within ECD_01133at 1198.177 kb on - strand, within ECD_01133at 1198.321 kb on + strandat 1198.322 kb on - strandat 1198.381 kb on + strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction lambda1857_phage_12.5_MOI
remove
1,197,212 - -2.3
1,197,224 - +2.3
1,197,581 - ECD_01132 0.84 -0.1
1,197,715 - +1.4
1,197,801 + ECD_01133 0.21 -0.2
1,197,802 - ECD_01133 0.21 +0.4
1,197,802 - ECD_01133 0.21 +1.5
1,197,802 - ECD_01133 0.21 +1.8
1,197,804 - ECD_01133 0.21 +2.6
1,197,804 - ECD_01133 0.21 +1.6
1,197,804 - ECD_01133 0.21 -1.9
1,197,804 - ECD_01133 0.21 +1.2
1,197,804 - ECD_01133 0.21 +0.3
1,197,804 - ECD_01133 0.21 +1.4
1,198,177 - ECD_01133 0.78 -0.8
1,198,321 + +0.0
1,198,322 - -0.2
1,198,381 + +1.6

Or see this region's nucleotide sequence