Strain Fitness in Escherichia coli BL21 around ECD_01126

Experiment: lambda1857_phage_12.5_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01125 and ECD_01126 are separated by 48 nucleotidesECD_01126 and ECD_01127 are separated by 75 nucleotides ECD_01125: ECD_01125 - peptidase T, at 1,189,212 to 1,190,438 _01125 ECD_01126: ECD_01126 - 50S ribosomal protein L16 arginine hydroxylase; 2-oxoglutarate oxygenase, at 1,190,487 to 1,191,608 _01126 ECD_01127: ECD_01127 - sensory histidine kinase in two-component regulatory system with PhoP, at 1,191,684 to 1,193,144 _01127 Position (kb) 1190 1191 1192Strain fitness (log2 ratio) -2 -1 0 1 2at 1189.610 kb on + strand, within ECD_01125at 1190.125 kb on + strand, within ECD_01125at 1190.166 kb on + strand, within ECD_01125at 1190.169 kb on + strand, within ECD_01125at 1190.169 kb on + strand, within ECD_01125at 1190.351 kb on + strandat 1190.351 kb on + strandat 1190.654 kb on - strand, within ECD_01126at 1190.926 kb on + strand, within ECD_01126at 1190.927 kb on - strand, within ECD_01126at 1190.927 kb on - strand, within ECD_01126at 1191.378 kb on - strand, within ECD_01126at 1191.398 kb on - strand, within ECD_01126at 1191.630 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction lambda1857_phage_12.5_MOI
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1,189,610 + ECD_01125 0.32 +0.2
1,190,125 + ECD_01125 0.74 -0.4
1,190,166 + ECD_01125 0.78 +0.0
1,190,169 + ECD_01125 0.78 -1.3
1,190,169 + ECD_01125 0.78 -2.2
1,190,351 + +0.8
1,190,351 + -0.7
1,190,654 - ECD_01126 0.15 -1.1
1,190,926 + ECD_01126 0.39 +0.9
1,190,927 - ECD_01126 0.39 -1.8
1,190,927 - ECD_01126 0.39 +2.6
1,191,378 - ECD_01126 0.79 -0.5
1,191,398 - ECD_01126 0.81 +1.7
1,191,630 - -0.9

Or see this region's nucleotide sequence