Strain Fitness in Escherichia coli BL21 around ECD_00972

Experiment: lambda1857_phage_12.5_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00971 and ECD_00972 are separated by 94 nucleotidesECD_00972 and ECD_00973 overlap by 4 nucleotidesECD_00973 and ECD_00974 are separated by 90 nucleotides ECD_00971: ECD_00971 - 23S rRNA m(5)C1962 methyltransferase, SAM-dependent, at 1,033,868 to 1,035,058 _00971 ECD_00972: ECD_00972 - weak acylphosphatase, at 1,035,153 to 1,035,431 _00972 ECD_00973: ECD_00973 - mnm(5)-s(2)U34-tRNA 2-thiolation sulfurtransferase, at 1,035,428 to 1,035,757 _00973 ECD_00974: ECD_00974 - Modulator of FtsH protease, inner membrane protein, at 1,035,848 to 1,036,507 _00974 Position (kb) 1035 1036Strain fitness (log2 ratio) -1 0 1 2at 1034.203 kb on + strand, within ECD_00971at 1034.203 kb on + strand, within ECD_00971at 1034.204 kb on - strand, within ECD_00971at 1034.204 kb on - strand, within ECD_00971at 1034.370 kb on + strand, within ECD_00971at 1034.573 kb on - strand, within ECD_00971at 1034.713 kb on + strand, within ECD_00971at 1034.714 kb on - strand, within ECD_00971at 1034.714 kb on - strand, within ECD_00971at 1034.755 kb on - strand, within ECD_00971at 1035.169 kb on + strandat 1035.935 kb on - strand, within ECD_00974at 1035.937 kb on - strand, within ECD_00974at 1035.937 kb on - strand, within ECD_00974at 1036.141 kb on - strand, within ECD_00974at 1036.141 kb on - strand, within ECD_00974

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Per-strain Table

Position Strand Gene LocusTag Fraction lambda1857_phage_12.5_MOI
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1,034,203 + ECD_00971 0.28 -0.6
1,034,203 + ECD_00971 0.28 -0.9
1,034,204 - ECD_00971 0.28 +0.3
1,034,204 - ECD_00971 0.28 -1.1
1,034,370 + ECD_00971 0.42 -1.0
1,034,573 - ECD_00971 0.59 +1.2
1,034,713 + ECD_00971 0.71 +0.8
1,034,714 - ECD_00971 0.71 +0.1
1,034,714 - ECD_00971 0.71 -0.2
1,034,755 - ECD_00971 0.74 +1.9
1,035,169 + -0.2
1,035,935 - ECD_00974 0.13 -1.6
1,035,937 - ECD_00974 0.13 -1.2
1,035,937 - ECD_00974 0.13 +0.5
1,036,141 - ECD_00974 0.44 +0.8
1,036,141 - ECD_00974 0.44 -1.5

Or see this region's nucleotide sequence