Experiment: lambda1857_phage_12.5_MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECD_00671 and ECD_00672 overlap by 7 nucleotides ECD_00672 and ECD_00673 overlap by 11 nucleotides ECD_00673 and ECD_00674 are separated by 35 nucleotides ECD_00674 and ECD_00675 overlap by 4 nucleotides
ECD_00671: ECD_00671 - putative allophanate hydrolase, subunit 1, at 702,157 to 702,813
_00671
ECD_00672: ECD_00672 - putative allophanate hydrolase, subunit 2, at 702,807 to 703,739
_00672
ECD_00673: ECD_00673 - UPF0271 family protein, at 703,729 to 704,463
_00673
ECD_00674: ECD_00674 - endonuclease VIII and 5-formyluracil/5-hydroxymethyluracil DNA glycosylase, at 704,499 to 705,290
_00674
ECD_00675: ECD_00675 - regulator of aidB expression; inner membrane protein, at 705,287 to 706,333
_00675
Position (kb) 703
704
705 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 702.758 kb on + strand at 702.758 kb on + strand at 702.758 kb on + strand at 702.758 kb on + strand at 702.761 kb on + strand at 702.788 kb on + strand at 702.834 kb on + strand at 702.834 kb on + strand at 702.834 kb on + strand at 702.834 kb on + strand at 702.834 kb on + strand at 702.834 kb on + strand at 702.835 kb on - strand at 702.835 kb on - strand at 702.836 kb on + strand at 703.055 kb on + strand, within ECD_00672 at 703.055 kb on + strand, within ECD_00672 at 703.112 kb on + strand, within ECD_00672 at 703.160 kb on + strand, within ECD_00672 at 703.283 kb on + strand, within ECD_00672 at 703.350 kb on + strand, within ECD_00672 at 703.439 kb on - strand, within ECD_00672 at 703.480 kb on + strand, within ECD_00672 at 703.602 kb on - strand, within ECD_00672 at 703.602 kb on - strand, within ECD_00672 at 703.605 kb on + strand, within ECD_00672 at 703.605 kb on + strand, within ECD_00672 at 704.056 kb on + strand, within ECD_00673 at 704.057 kb on - strand, within ECD_00673 at 704.111 kb on - strand, within ECD_00673 at 704.111 kb on - strand, within ECD_00673 at 704.304 kb on - strand, within ECD_00673 at 704.427 kb on + strand at 704.471 kb on + strand at 704.687 kb on - strand, within ECD_00674 at 704.810 kb on + strand, within ECD_00674 at 705.006 kb on + strand, within ECD_00674 at 705.006 kb on + strand, within ECD_00674 at 705.006 kb on + strand, within ECD_00674 at 705.155 kb on + strand, within ECD_00674 at 705.155 kb on + strand, within ECD_00674 at 705.156 kb on - strand, within ECD_00674 at 705.352 kb on + strand at 705.385 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction lambda1857_phage_12.5_MOI remove 702,758 + +0.4 702,758 + +1.7 702,758 + -0.8 702,758 + +0.1 702,761 + +0.5 702,788 + -2.1 702,834 + +1.8 702,834 + -1.3 702,834 + +1.4 702,834 + -0.7 702,834 + -2.3 702,834 + -1.0 702,835 - -1.0 702,835 - +0.0 702,836 + +0.3 703,055 + ECD_00672 0.27 +3.1 703,055 + ECD_00672 0.27 +0.6 703,112 + ECD_00672 0.33 +0.2 703,160 + ECD_00672 0.38 +2.1 703,283 + ECD_00672 0.51 +0.3 703,350 + ECD_00672 0.58 -0.2 703,439 - ECD_00672 0.68 -1.5 703,480 + ECD_00672 0.72 +0.4 703,602 - ECD_00672 0.85 -0.8 703,602 - ECD_00672 0.85 -0.4 703,605 + ECD_00672 0.86 +1.5 703,605 + ECD_00672 0.86 -2.8 704,056 + ECD_00673 0.44 +2.6 704,057 - ECD_00673 0.45 +2.9 704,111 - ECD_00673 0.52 -1.4 704,111 - ECD_00673 0.52 +2.1 704,304 - ECD_00673 0.78 -1.3 704,427 + -0.5 704,471 + +0.5 704,687 - ECD_00674 0.24 +0.7 704,810 + ECD_00674 0.39 +0.4 705,006 + ECD_00674 0.64 -3.1 705,006 + ECD_00674 0.64 -0.1 705,006 + ECD_00674 0.64 +0.3 705,155 + ECD_00674 0.83 -0.9 705,155 + ECD_00674 0.83 -1.0 705,156 - ECD_00674 0.83 -1.4 705,352 + -2.2 705,385 + -1.5
Or see this region's nucleotide sequence