Strain Fitness in Escherichia coli BL21 around ECD_00616

Experiment: lambda1857_phage_12.5_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00615 and ECD_00616 are separated by 59 nucleotidesECD_00616 and ECD_00617 are separated by 83 nucleotides ECD_00615: ECD_00615 - J domain-containing HscC co-chaperone; Hsc56, at 638,089 to 639,228 _00615 ECD_00616: ECD_00616 - Hsp70 family chaperone Hsc62; RpoD-binding transcription inhibitor, at 639,288 to 640,958 _00616 ECD_00617: ECD_00617 - ribonucleoside hydrolase 1, at 641,042 to 641,977 _00617 Position (kb) 639 640 641Strain fitness (log2 ratio) -2 -1 0 1 2at 638.371 kb on + strand, within ECD_00615at 638.453 kb on - strand, within ECD_00615at 638.542 kb on + strand, within ECD_00615at 638.542 kb on + strand, within ECD_00615at 638.542 kb on + strand, within ECD_00615at 638.625 kb on + strand, within ECD_00615at 638.637 kb on + strand, within ECD_00615at 638.688 kb on - strand, within ECD_00615at 638.720 kb on + strand, within ECD_00615at 638.720 kb on + strand, within ECD_00615at 638.720 kb on + strand, within ECD_00615at 638.746 kb on + strand, within ECD_00615at 638.754 kb on + strand, within ECD_00615at 638.949 kb on - strand, within ECD_00615at 638.988 kb on + strand, within ECD_00615at 638.991 kb on + strand, within ECD_00615at 639.188 kb on + strandat 639.223 kb on - strandat 639.874 kb on + strand, within ECD_00616at 640.258 kb on + strand, within ECD_00616at 640.259 kb on - strand, within ECD_00616at 640.259 kb on - strand, within ECD_00616at 640.476 kb on - strand, within ECD_00616at 640.666 kb on - strand, within ECD_00616at 641.296 kb on + strand, within ECD_00617at 641.476 kb on - strand, within ECD_00617at 641.480 kb on - strand, within ECD_00617at 641.518 kb on + strand, within ECD_00617at 641.593 kb on + strand, within ECD_00617at 641.841 kb on - strand, within ECD_00617at 641.878 kb on + strand, within ECD_00617at 641.879 kb on - strand, within ECD_00617at 641.921 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction lambda1857_phage_12.5_MOI
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638,371 + ECD_00615 0.25 -2.3
638,453 - ECD_00615 0.32 -1.6
638,542 + ECD_00615 0.40 -0.9
638,542 + ECD_00615 0.40 +0.3
638,542 + ECD_00615 0.40 +1.4
638,625 + ECD_00615 0.47 +1.6
638,637 + ECD_00615 0.48 +1.5
638,688 - ECD_00615 0.53 -2.6
638,720 + ECD_00615 0.55 -0.9
638,720 + ECD_00615 0.55 +1.0
638,720 + ECD_00615 0.55 +0.9
638,746 + ECD_00615 0.58 +0.8
638,754 + ECD_00615 0.58 +1.1
638,949 - ECD_00615 0.75 +0.5
638,988 + ECD_00615 0.79 +0.3
638,991 + ECD_00615 0.79 +2.5
639,188 + +2.7
639,223 - +0.3
639,874 + ECD_00616 0.35 -0.1
640,258 + ECD_00616 0.58 +0.6
640,259 - ECD_00616 0.58 +2.7
640,259 - ECD_00616 0.58 +0.7
640,476 - ECD_00616 0.71 +0.5
640,666 - ECD_00616 0.82 -1.1
641,296 + ECD_00617 0.27 +0.0
641,476 - ECD_00617 0.46 -0.1
641,480 - ECD_00617 0.47 -1.3
641,518 + ECD_00617 0.51 +1.0
641,593 + ECD_00617 0.59 -0.7
641,841 - ECD_00617 0.85 +2.0
641,878 + ECD_00617 0.89 +0.1
641,879 - ECD_00617 0.89 +1.6
641,921 + +1.3

Or see this region's nucleotide sequence