Strain Fitness in Escherichia coli BL21 around ECD_00586

Experiment: lambda1857_phage_12.5_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00584 and ECD_00585 overlap by 4 nucleotidesECD_00585 and ECD_00586 are separated by 14 nucleotidesECD_00586 and ECD_00587 are separated by 85 nucleotidesECD_00587 and ECD_00588 overlap by 82 nucleotidesECD_00588 and ECD_00589 are separated by 132 nucleotides ECD_00584: ECD_00584 - citrate lyase, citryl-ACP lyase (beta) subunit, at 609,412 to 610,320 _00584 ECD_00585: ECD_00585 - citrate lyase, acyl carrier (gamma) subunit, at 610,317 to 610,613 _00585 ECD_00586: ECD_00586 - citrate lyase ligase; [citrate [pro-3S]-lyase] ligase, at 610,628 to 611,686 _00586 ECD_00587: ECD_00587 - IS1 protein InsB, at 611,772 to 612,275 _00587 ECD_00588: ECD_00588 - IS1 protein InsA, at 612,194 to 612,469 _00588 ECD_00589: ECD_00589 - sensory histidine kinase in two-component regulatory system with CitB, at 612,602 to 614,260 _00589 Position (kb) 610 611 612Strain fitness (log2 ratio) -2 -1 0 1 2 3at 609.822 kb on - strand, within ECD_00584at 609.981 kb on + strand, within ECD_00584at 610.294 kb on - strandat 610.448 kb on - strand, within ECD_00585at 610.557 kb on + strand, within ECD_00585at 610.558 kb on - strand, within ECD_00585at 610.926 kb on - strand, within ECD_00586at 611.017 kb on - strand, within ECD_00586at 611.017 kb on - strand, within ECD_00586at 611.046 kb on + strand, within ECD_00586at 611.046 kb on + strand, within ECD_00586at 611.046 kb on + strand, within ECD_00586at 611.150 kb on + strand, within ECD_00586at 611.199 kb on + strand, within ECD_00586at 611.515 kb on + strand, within ECD_00586at 611.515 kb on + strand, within ECD_00586at 611.573 kb on + strand, within ECD_00586at 611.690 kb on - strandat 611.690 kb on - strandat 611.694 kb on - strandat 612.528 kb on - strandat 612.531 kb on - strandat 612.560 kb on + strandat 612.614 kb on - strandat 612.623 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction lambda1857_phage_12.5_MOI
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609,822 - ECD_00584 0.45 +0.5
609,981 + ECD_00584 0.63 -1.8
610,294 - -0.6
610,448 - ECD_00585 0.44 +0.6
610,557 + ECD_00585 0.81 +1.0
610,558 - ECD_00585 0.81 -1.8
610,926 - ECD_00586 0.28 +0.9
611,017 - ECD_00586 0.37 +1.3
611,017 - ECD_00586 0.37 +1.2
611,046 + ECD_00586 0.39 -1.3
611,046 + ECD_00586 0.39 +0.1
611,046 + ECD_00586 0.39 +1.4
611,150 + ECD_00586 0.49 +3.2
611,199 + ECD_00586 0.54 -0.9
611,515 + ECD_00586 0.84 +0.1
611,515 + ECD_00586 0.84 -1.3
611,573 + ECD_00586 0.89 +0.8
611,690 - -0.9
611,690 - -2.7
611,694 - -0.6
612,528 - -1.6
612,531 - +0.7
612,560 + +0.1
612,614 - +0.9
612,623 - +0.0

Or see this region's nucleotide sequence