Experiment: lambda1857_phage_12.5_MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECD_00383 and ECD_00384 are separated by 461 nucleotides ECD_00384 and ECD_00385 are separated by 43 nucleotides ECD_00385 and ECD_00386 are separated by 304 nucleotides
ECD_00383: ECD_00383 - cytochrome o ubiquinol oxidase subunit II, at 418,345 to 419,292
_00383
ECD_00384: ECD_00384 - muropeptide transporter, at 419,754 to 421,229
_00384
ECD_00385: ECD_00385 - putative lipoprotein, at 421,273 to 421,851
_00385
ECD_00386: ECD_00386 - stationary-phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC, at 422,156 to 422,473
_00386
Position (kb) 419
420
421
422 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 419.000 kb on - strand, within ECD_00383 at 419.000 kb on - strand, within ECD_00383 at 419.004 kb on + strand, within ECD_00383 at 419.005 kb on - strand, within ECD_00383 at 419.005 kb on - strand, within ECD_00383 at 419.005 kb on - strand, within ECD_00383 at 419.009 kb on - strand, within ECD_00383 at 419.060 kb on + strand, within ECD_00383 at 419.061 kb on - strand, within ECD_00383 at 419.061 kb on - strand, within ECD_00383 at 419.064 kb on + strand, within ECD_00383 at 419.064 kb on + strand, within ECD_00383 at 419.064 kb on + strand, within ECD_00383 at 419.065 kb on - strand, within ECD_00383 at 419.204 kb on - strand at 419.234 kb on - strand at 419.234 kb on - strand at 419.249 kb on - strand at 419.358 kb on - strand at 419.362 kb on + strand at 419.415 kb on - strand at 419.415 kb on - strand at 419.428 kb on + strand at 419.429 kb on - strand at 419.827 kb on - strand at 419.902 kb on - strand, within ECD_00384 at 420.005 kb on + strand, within ECD_00384 at 420.008 kb on - strand, within ECD_00384 at 420.072 kb on + strand, within ECD_00384 at 420.076 kb on + strand, within ECD_00384 at 420.076 kb on + strand, within ECD_00384 at 420.077 kb on - strand, within ECD_00384 at 420.131 kb on - strand, within ECD_00384 at 420.142 kb on - strand, within ECD_00384 at 420.191 kb on + strand, within ECD_00384 at 420.192 kb on - strand, within ECD_00384 at 420.195 kb on - strand, within ECD_00384 at 420.195 kb on - strand, within ECD_00384 at 420.281 kb on - strand, within ECD_00384 at 420.398 kb on - strand, within ECD_00384 at 420.527 kb on + strand, within ECD_00384 at 420.529 kb on + strand, within ECD_00384 at 420.539 kb on + strand, within ECD_00384 at 420.539 kb on + strand, within ECD_00384 at 420.668 kb on + strand, within ECD_00384 at 420.700 kb on + strand, within ECD_00384 at 420.725 kb on + strand, within ECD_00384 at 420.955 kb on - strand, within ECD_00384 at 420.955 kb on - strand, within ECD_00384 at 420.958 kb on - strand, within ECD_00384 at 421.016 kb on - strand, within ECD_00384 at 421.055 kb on - strand, within ECD_00384 at 421.100 kb on + strand at 421.125 kb on + strand at 421.125 kb on + strand at 421.126 kb on - strand at 421.238 kb on - strand at 421.324 kb on - strand at 421.324 kb on - strand at 421.324 kb on - strand at 421.407 kb on - strand, within ECD_00385 at 421.491 kb on - strand, within ECD_00385 at 421.521 kb on - strand, within ECD_00385 at 421.521 kb on - strand, within ECD_00385 at 421.575 kb on - strand, within ECD_00385 at 421.575 kb on - strand, within ECD_00385 at 421.717 kb on + strand, within ECD_00385 at 421.758 kb on + strand, within ECD_00385 at 421.813 kb on - strand at 421.813 kb on - strand at 421.813 kb on - strand at 421.873 kb on + strand at 421.874 kb on - strand at 421.874 kb on - strand at 421.942 kb on + strand at 422.032 kb on + strand at 422.066 kb on + strand at 422.066 kb on + strand at 422.067 kb on - strand at 422.076 kb on + strand at 422.175 kb on + strand at 422.220 kb on + strand, within ECD_00386
Per-strain Table
Position Strand Gene LocusTag Fraction lambda1857_phage_12.5_MOI remove 419,000 - ECD_00383 0.69 -1.9 419,000 - ECD_00383 0.69 -1.1 419,004 + ECD_00383 0.70 -1.8 419,005 - ECD_00383 0.70 -2.8 419,005 - ECD_00383 0.70 -2.5 419,005 - ECD_00383 0.70 -2.4 419,009 - ECD_00383 0.70 -4.5 419,060 + ECD_00383 0.75 -2.7 419,061 - ECD_00383 0.76 -4.2 419,061 - ECD_00383 0.76 -2.9 419,064 + ECD_00383 0.76 -3.0 419,064 + ECD_00383 0.76 +0.6 419,064 + ECD_00383 0.76 -0.0 419,065 - ECD_00383 0.76 -2.8 419,204 - -3.5 419,234 - -3.1 419,234 - -3.1 419,249 - -4.1 419,358 - -2.2 419,362 + -4.0 419,415 - -3.4 419,415 - -0.3 419,428 + -0.1 419,429 - -0.8 419,827 - +1.1 419,902 - ECD_00384 0.10 +0.1 420,005 + ECD_00384 0.17 +0.7 420,008 - ECD_00384 0.17 +0.5 420,072 + ECD_00384 0.22 -2.5 420,076 + ECD_00384 0.22 +0.8 420,076 + ECD_00384 0.22 -0.6 420,077 - ECD_00384 0.22 -3.6 420,131 - ECD_00384 0.26 -1.0 420,142 - ECD_00384 0.26 +0.1 420,191 + ECD_00384 0.30 -2.6 420,192 - ECD_00384 0.30 +0.5 420,195 - ECD_00384 0.30 +0.3 420,195 - ECD_00384 0.30 +0.0 420,281 - ECD_00384 0.36 +1.0 420,398 - ECD_00384 0.44 -0.9 420,527 + ECD_00384 0.52 +0.9 420,529 + ECD_00384 0.53 +0.0 420,539 + ECD_00384 0.53 -2.7 420,539 + ECD_00384 0.53 -0.1 420,668 + ECD_00384 0.62 -0.9 420,700 + ECD_00384 0.64 -1.7 420,725 + ECD_00384 0.66 +1.1 420,955 - ECD_00384 0.81 +0.2 420,955 - ECD_00384 0.81 -1.7 420,958 - ECD_00384 0.82 -4.0 421,016 - ECD_00384 0.86 -3.0 421,055 - ECD_00384 0.88 +1.0 421,100 + -0.9 421,125 + -0.6 421,125 + +0.1 421,126 - -0.5 421,238 - +1.6 421,324 - +0.9 421,324 - -0.9 421,324 - -0.4 421,407 - ECD_00385 0.23 -1.1 421,491 - ECD_00385 0.38 -0.5 421,521 - ECD_00385 0.43 +3.3 421,521 - ECD_00385 0.43 -1.1 421,575 - ECD_00385 0.52 +0.6 421,575 - ECD_00385 0.52 +2.6 421,717 + ECD_00385 0.77 -1.0 421,758 + ECD_00385 0.84 -0.4 421,813 - -0.6 421,813 - +1.2 421,813 - +0.7 421,873 + -0.2 421,874 - -0.3 421,874 - -0.6 421,942 + +1.0 422,032 + +0.6 422,066 + -0.2 422,066 + -0.4 422,067 - -0.4 422,076 + +0.6 422,175 + +1.0 422,220 + ECD_00386 0.20 -0.8
Or see this region's nucleotide sequence